Title: Effect of sevoflurance postconditioning on cardiac function and mitochondrial of isolated rat heart with acute myocardial ischemia-reperfusion injury
Abstract: Aim To investigate the effect of sevoflurane postconditioning on isolated rat hearts with acute myocardial ischemia reperfusion injury.Methods 48 SD rats were randomly divided into 4 groups(n=12):sham(150 min perfusion);ischemia reperfusion(30min ischemia);4 cycles of ischemia postconditioning(20 s of each);2.57 V/V sevoflurane postconditioning(1.5 minimum alveolar concentration),using Langendorff(apparatus.Except sham group,all groups subjected to 30 min ischemia followed by 90 min reperfusion.Post group was subjected to 4 cycles of 20 s reperfusion/ischemia after 30 min ischemia;10 min of washout was administered immediately after Sevo group was induced by 5 min 2.57 vol % sevoflurane at onset of reperfusion.Recording Left ventricular diastolic pressure(LVEDP),Left ventricular developed pressure(LVDP),Heart rate(HR),Coronary flow(CF),+dp/dt and-dp/dt at 30 min of equilibrium,30,60,90 min of reperfusion respectively.Acute infarct size was measured using 1% triphenyltetrazolium chloride staining.Configuration of mitochondria was observed by electronmicroscope.Western blot analysis was used to determine contents of cytosolic and mitochondrial cytochrome C at the end of reperfusion.Results No differences in baseline hemodynamics were observed among the experimental groups(P0.05).After reperfusion,Sevo group and Post group resulted in a significant increase(P0.05) in hemodynamics compared with I/R group.Sevo group and Post group significantly(P0.05) reduced infarct size(21%+/-7%,23%+/-6%,respectively) VS I/R group(39%+/-9%).Sevo group and Post group improved configuration of mitochodria and significantly(P0.05) reduced the release of cytochrome C from mitochodria into cytosolic.Conclusion Sevoflurance postconditioning effectively protects against reperfusion damage similar to ischemia postconditionding.The mechanism might be involved in decreasing the damage of mitochodria.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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