Title: GREEN FLUORESCENT PROTEIN AS A VISUAL SELECTION MARKER FOR COTTON TRANSFORMATION
Abstract:Transgenic cotton plants have been produced by vacuum infiltration, particle bombardment, and Agrobacterium-mediated transformation. Most transgenic plants have co-introduced antibiotic or herbicide r...Transgenic cotton plants have been produced by vacuum infiltration, particle bombardment, and Agrobacterium-mediated transformation. Most transgenic plants have co-introduced antibiotic or herbicide resistant genes which serve no useful purpose after the transgenic plants are produced. The presence of antibiotic and herbicide resistance marker genes in transgenic plants has raised public concern about ecological and food safety perspective of these genes (Puchta 2003). Generating a transformation system without antibiotic resistant and herbicide resistant genes would certainly contribute to public acceptance of transgenic cotton. The green fluorescent protein (GFP) gene has become a very effective marker gene for use in plant genetic transformation. Recent reports on GFP showed that apart from being used as a visual reporter gene instead of β-Glucuronidase (GUS) (Jordan 2000), it could also be used as a visual selection marker gene instead of chemical based antibiotic selection marker genes. Because of it, for the first time in plant transformation, researchers have at their disposal a universal, in vivo, and realtime transgenic visible marker (Stewart 2001). The GFP visual selection efficiency is much higher than antibiotic selection and can greatly reduce the time involved in transgenic plant production. GFP visual selection made transformation possible without antibiotic resistant marker genes without reducing transformation efficiency since there is no harmful effect on plants with the GFP gene. Establishment of GFP gene visual selection transformation system in cotton will provide a new transformation protocol in cotton.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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