Title: Expression of micro RNA-200c in pancreatic cancer stem cells and its significance
Abstract: Objective:To investigate the expression and action of microRNA-200c(miRNA-200c) in pancreatic cancer stem cells.Methods:Pancreatic cancer stem cells were sorted from human pancreatic cancer PANC-1 cells by FACS using CD24~+CD44~+ESA~+ as marker,and their stem cell properties were assessed by xenograft tumor assay in NOD/SCID mice.The miRNA-200 c expression and invasion ability in PANC-1 cells,pancreatic cancer stem cells and pancreatic cancer stem cells transfected with miRNA-200 c precursor sequence or negative control sequence were determined by RFQ-PCR method and Transwell invasion assay,respectively.Results:The CD24~+CD44~+ESA~+cells(accounting for 0.8%) sorted from PANC-1 cells presented tumor stem cell properties,and the volume of the xenograft tumor after their subcutaneous transplantation in mice was significantly larger than that after PANC-1 cells transplanted at the same time[(1 725.14±261.29) mm3 vs.(479.65±99.67) mm3,P0.05].In pancreatic cancer stem cells compared with PANC-1 cells,the miRNA-200 c expression level was significantly decreased(0.15±0.01 vs.1.00±0.09,P0.05),and transmembrane cell number was significantly increased(321±7.62 vs.70±16.47,P0.05),but the miRNA-200 c expression level was significantly increased and transmembrane cell number was significantly decreased in pancreatic cancer stem cells after transfection with miRNA-200 c precursor sequence(both P0.05).Conclusion:MiRNA-200 c expression is reduced in pancreatic cancer stem cells,and miRNA-200 c has inhibitory effect on growth and invasiveness of pancreatic cancer stem cells.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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