Title: Therapeutic effect of ursolic acid on fetal development in pregnant rats with gestational diabetes mellitus via AGEs‐RAGE signaling pathway
Abstract: Journal of Food BiochemistryVolume 45, Issue 4 e13651 FULL ARTICLE Therapeutic effect of ursolic acid on fetal development in pregnant rats with gestational diabetes mellitus via AGEs-RAGE signaling pathway Senge Dai, Senge Dai Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China Contribution: Conceptualization, Data curation, Formal analysisSearch for more papers by this authorXiaoyan Meng, Xiaoyan Meng Department of Obstetrics and Gynecology, Suzhou Wuzhong People's Hospital, Suzhou, China Contribution: Data curation, Formal analysis, Funding acquisition, Writing - original draftSearch for more papers by this authorXiaqin Cai, Xiaqin Cai Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China Contribution: Investigation, Writing - original draft, Writing - review & editingSearch for more papers by this authorChun Yuan, Chun Yuan Disinfection Supply Center, The First Hospital of HuBei WuHan, Wuhan, China Contribution: Methodology, Project administration, Writing - original draftSearch for more papers by this authorZhongmei Zhao, Zhongmei Zhao Department of Gynecology, Yantai Municipal Laiyang Central Hospital, Laiyang, China Contribution: Methodology, Project administrationSearch for more papers by this authorLi Zhong, Li Zhong Department of Gynecology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China Contribution: Formal analysis, Resources, Software, Writing - original draft, Writing - review & editingSearch for more papers by this authorYongmei Shi, Yongmei Shi Department of Obstetrics and Gynecology, Tongzhou Maternal & Child Health Hospital of Beijing, Beijing, China Contribution: Formal analysis, Funding acquisition, Visualization, Writing - original draft, Writing - review & editingSearch for more papers by this authorFengling Yin, Corresponding Author Fengling Yin [email protected] orcid.org/0000-0002-8804-9874 Department of Gynecology, Xuzhou Central Hospital, XuZhou Clinical School of Xuzhou Medical University, The Xuzhou School of Clinical Medicine of Nanjing Medical University, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Affiliated Hospital of Southeast University, Xuzhou, China Correspondence Fengling Yin, Department of Gynecology, Xuzhou Central Hospital, XuZhou Clinical School of Xuzhou Medical University, The Xuzhou School of Clinical Medicine of Nanjing Medical University, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Affiliated Hospital of Southeast University, Xuzhou 221009, China. Email: [email protected] Contribution: Conceptualization, Writing - original draft, Writing - review & editingSearch for more papers by this author Senge Dai, Senge Dai Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China Contribution: Conceptualization, Data curation, Formal analysisSearch for more papers by this authorXiaoyan Meng, Xiaoyan Meng Department of Obstetrics and Gynecology, Suzhou Wuzhong People's Hospital, Suzhou, China Contribution: Data curation, Formal analysis, Funding acquisition, Writing - original draftSearch for more papers by this authorXiaqin Cai, Xiaqin Cai Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China Contribution: Investigation, Writing - original draft, Writing - review & editingSearch for more papers by this authorChun Yuan, Chun Yuan Disinfection Supply Center, The First Hospital of HuBei WuHan, Wuhan, China Contribution: Methodology, Project administration, Writing - original draftSearch for more papers by this authorZhongmei Zhao, Zhongmei Zhao Department of Gynecology, Yantai Municipal Laiyang Central Hospital, Laiyang, China Contribution: Methodology, Project administrationSearch for more papers by this authorLi Zhong, Li Zhong Department of Gynecology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China Contribution: Formal analysis, Resources, Software, Writing - original draft, Writing - review & editingSearch for more papers by this authorYongmei Shi, Yongmei Shi Department of Obstetrics and Gynecology, Tongzhou Maternal & Child Health Hospital of Beijing, Beijing, China Contribution: Formal analysis, Funding acquisition, Visualization, Writing - original draft, Writing - review & editingSearch for more papers by this authorFengling Yin, Corresponding Author Fengling Yin [email protected] orcid.org/0000-0002-8804-9874 Department of Gynecology, Xuzhou Central Hospital, XuZhou Clinical School of Xuzhou Medical University, The Xuzhou School of Clinical Medicine of Nanjing Medical University, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Affiliated Hospital of Southeast University, Xuzhou, China Correspondence Fengling Yin, Department of Gynecology, Xuzhou Central Hospital, XuZhou Clinical School of Xuzhou Medical University, The Xuzhou School of Clinical Medicine of Nanjing Medical University, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Affiliated Hospital of Southeast University, Xuzhou 221009, China. Email: [email protected] Contribution: Conceptualization, Writing - original draft, Writing - review & editingSearch for more papers by this author First published: 15 February 2021 https://doi.org/10.1111/jfbc.13651Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract To investigate the effect of ursolic acid on the fetal development of gestational diabetes mellitus (GDM) caused by streptozotocin (STZ) and explore the potential mechanism for it. For the current experimental research, SD rats (pregnant animal) were used. STZ has been used to cause the diabetes mellitus in pregnant rats. Rats with evolved GDM were randomly divided and ursolic acid was given to pregnant rats in the experimental period up to 19 days in a dose-dependent manner. Blood samples and fetal rats of all group rats were collected at 19 days (pregnant rats), fetal rats and placental rats were weighted and the blood glucose, plasma insulin, C-peptide, and lipid parameters of pregnant women were estimated prior to delivery. Advanced serum glycation end-products (AGEs) were estimated at regular intervals in the heart and brain of pregnant rats. Monocyte Chemoattractant Protein-1 (MCP-1), NADPH oxidase 2 (Nox2), Role of advanced glycation end product (RAGE), Vascular endothelial growth factor (VEGF), p65, and vascular cell adhesion molecule 1 (VCAM-1) mRNA expression were estimated in the placenta. STZ-induced GDM pregnant rats showed significantly decreased placental weight and weight of fetal rats and dose-dependent ursolic acid treatment (p < .001) improved placental weight and weight of fetal rats at dose-dependent levels. After the ursolic acid treatment, serum blood glucose and lipid level were improved especially fasting blood glucose (FBG), high density lipoprotein (HDL), hepatic glycogen, fasting insulin (FINS), and serum insulin level and reached near to the normal control group rats. The antioxidant level of pancreas and liver were significantly (p < .001) reduced by the dose-dependent treatment of ursolic acid. Treatment with Ursolic acid moderately but not significantly decreases the risk of fetal development defects relative to the GDM group. The potential effect on fetal development in Pregnant Rats with Gestational Diabetes Mellitus via AGEs-RAGE signaling pathway was shown by Ursolic acid. Practical applications As we know that the gestational diabetes mellitus increases worldwide day by day. In the current experimental study, we try to examine the gestational diabetic effect of ursolic acid. The finding of the current study showed the gestational diabetic protective effect in the female rats via AGEs-RAGE signaling pathway. The result showed the antioxidant, anti-inflammatory, and biochemical parameters. On the basis of the result, we can say that the ursolic acid can be the protective drug for treatment of gestational diabetes mellitus. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Citing Literature Volume45, Issue4April 2021e13651 RelatedInformation