Abstract: Today, development of rapid and sensitive methods for direct detection of foodborne pathogens appeared as crucial matter due to their impact on human health. In this manner, graphene-based nanomaterials have received much attention as reliable electrochemical biosensors due to their exceptional combination of intrinsic properties such as high conductivity, stability and biocompatibility. The scope of this chapter is to provide a brief history of the electrochemical biosensors used for the detection of microbial pathogens and recent progress of graphene used in electrochemical biosensors for foodborne pathogens detection.