Publication Information

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2175, 2549, 3418, 3421, 3499, 4298], 'decorated': [1051], 'with': [1052, 1070, 1125, 1157, 1217, 1237, 1275, 1318, 1527, 1538, 1567, 1594, 1602, 1814, 1827, 1861, 1913, 1924, 1959, 2053, 2094, 2157, 2207, 2368, 2519, 2534, 2560, 2576, 2770, 2823, 2837, 2895, 2996, 3146, 3234, 3361, 3757, 4153], 'over': [1053, 1307], '170': [1054], 'types': [1055], 'chemical': [1057], 'modifications.': [1058], 'Many': [1059], 'modifications': [1060, 1314, 1366, 1381, 1473], 'in': [1061, 1152, 1184, 1246, 1297, 1306, 1399, 1619, 1640, 1762, 1781, 1856, 1887, 1996, 2075, 2107, 2177, 2187, 2195, 2328, 2339, 2355, 2417, 2433, 2469, 2491, 2499, 2512, 2524, 2609, 2662, 2686, 2703, 2721, 2764, 2975, 3019, 3035, 3040, 3084, 3097, 3106, 3144, 3186, 3204, 3267, 3300, 3312, 3327, 3349, 3357, 3385, 3402, 3450, 3458, 3466, 3478, 3514, 3524, 3567, 3615, 3636, 3726, 3767, 3782, 3845, 3886, 3917, 4012, 4021, 4041, 4091, 4184, 4201, 4217, 4248], 'mRNA,': [1062, 2181], 'm6A': [1064, 1421], 'm5C,': [1066], 'have': [1067, 1315, 1447, 1748], 'been': [1068, 1316, 1608, 1691, 3394], 'associated': [1069, 1317, 2052, 2575], 'critical': [1071], 'cellular': [1072, 1320], 'functions': [1073, 1083, 1321], 'under': [1074, 1322], 'physiological': [1075, 1323], 'and/or': [1076, 1159, 1324, 1863, 2719], 'pathological': [1077, 1325], 'conditions.': [1078], 'To': [1079, 1335, 1785, 1991, 2098, 2269, 2795, 2948, 3063, 3277, 3317, 4157], 'understand': [1080], 'biological': [1082, 3209, 3578], 'these': [1085, 1107, 3154, 3570], 'modifications,': [1086, 3595, 4254], 'it': [1087, 1793, 2978], 'vital': [1089], 'identify': [1091, 1375, 1798, 2270, 2630], 'that': [1094, 1163, 1194, 1897, 2210, 2603, 2632, 2981, 3680, 3694, 3761, 3947], 'modulate': [1095], 'rate.': [1098, 4156], 'However,': [1099, 1745, 3025, 3251], 'high-throughput': [1101, 1258, 1490], 'so': [1110], 'far': [1111], 'lacking.': [1112, 1476], 'Here,': [1113, 1802], 'we': [1114, 1139, 1192, 1803, 1835, 1895, 1934, 2624, 2647, 3125, 3225, 3283, 3674, 4108, 4140, 4169, 4255, 4289], 'report': [1115, 1804], 'such': [1116, 1313, 3830, 4129, 4224], 'combining': [1119, 1213, 1526, 1807, 2214], 'pooled': [1120, 1214, 1272, 1599, 1808, 1921], 'CRISPR': [1121, 1130, 1215, 1273, 1496, 1530, 1600, 1809, 1818, 1911, 1922], 'screen': [1122, 1216, 1274, 1467, 1497, 1500, 1531, 1601, 1810], 'reporters': [1124], 'readout,': [1128, 1816, 1927, 1933], 'termed': [1129, 1817], 'integrated': [1131, 1249, 1819], 'gRNA': [1132, 1250, 1820, 1839, 1989, 1998, 2022, 2055, 2108, 2130, 2160, 2166, 2225, 2249, 2371, 2378, 2439, 2784, 2806, 2874, 3068], 'reporter': [1134, 1220, 1252, 1813, 1822, 1829, 2046, 2105, 2151, 2180, 2217, 2267, 2332, 2398, 2419, 2435, 2459, 2573, 2778, 2916, 2931, 3099, 3269], 'sequencing': [1135, 1151, 1652, 1823, 2034, 2660, 2879, 2887, 3445], '(CIGAR-seq).': [1136, 1824], 'Using': [1137, 1825], 'discovered': [1140, 1228, 1280, 1425, 1643], 'NSUN6': [1141, 1158, 1164, 1226, 1278, 1287, 1847, 1862, 1867, 2064, 2305, 2312, 2323, 2592, 2720, 2732, 3011, 3038, 3070, 3089, 3127, 3147, 3157, 3173, 3185, 3195, 3203, 3285, 3382, 3467, 3481, 3504, 3533, 3548, 3587, 3598, 3626, 3685, 3699, 4002], 'as': [1142, 1189, 1229, 1281, 1576, 1848, 1892, 2065, 2313, 2319, 2321, 2359, 2460, 2462, 2529, 2593, 2664, 2666, 2734, 2736, 2776, 2992, 3158, 3396, 3565, 3649, 3660, 3688, 3690, 3807, 4283], 'm5C': [1146, 1173, 1182, 1233, 1244, 1285, 1295, 1638, 1688, 1760, 1783, 1832, 1851, 1876, 1885, 1931, 1957, 1982, 1994, 2019, 2045, 2077, 2092, 2101, 2127, 2150, 2173, 2200, 2216, 2246, 2262, 2316, 2326, 2331, 2337, 2353, 2393, 2412, 2464, 2495, 2510, 2517, 2569, 2607, 2635, 2645, 2682, 2701, 2773, 2821, 2871, 2912, 2926, 2944, 3002, 3018, 3033, 3080, 3095, 3104, 3135, 3163, 3247, 3265, 3275, 3281, 3297, 3310, 3325, 3345, 3353, 3370, 3416, 3441, 3448, 3455, 3512, 3539, 3555, 3563, 3594, 3621, 3717, 3724, 3759, 3780, 3791, 3834, 3843, 3857, 3866, 3877, 3883, 3913, 3945, 3966, 3974, 3996, 4010, 4019, 4039, 4049, 4065, 4080, 4089, 4106, 4118, 4149, 4164, 4187, 4195], 'methyltransferase.': [1147, 1286, 2317, 3164], 'Subsequent': [1148], 'bisulfite': [1150, 1651, 2007, 2115, 2659, 3444, 3805, 4123], 'HAP1': [1153, 1857, 2203, 2391, 2471, 2492, 2501, 2652, 2688, 2722, 2745, 2832, 3188, 3205, 3288, 3302, 3328, 3451, 3484, 3515, 3602, 3892, 3918], 'cells': [1154, 1858, 2204, 2526, 2653, 2689, 2705, 2723, 2746, 2833, 2850, 3042, 3145, 3289, 3314, 3603, 3893], 'without': [1155, 1859, 2161, 2372], 'or': [1156, 1860, 2032, 2141, 2376, 3178, 3627], 'NSUN2': [1160, 1166, 1289, 1753, 1864, 1869, 2170, 2380, 2407, 2421, 2452, 2478, 2488, 2554, 2614, 2649, 2718, 2730, 3535, 3550, 3585, 3596, 3628, 4004, 4286], 'knockout': [1161, 1754, 1865, 2189, 2260, 2555, 2615, 2650, 2739, 2743, 3252, 3286, 3505], 'showed': [1162, 1866, 2507, 2698, 2941, 3306, 3462, 3681, 3948, 3963], 'worked': [1167, 1870], 'on': [1168, 1363, 1431, 1455, 1871, 1950, 2082, 2396, 2457, 2637, 2750, 2963, 3246, 3335, 3625, 3683, 4000, 4189], 'non-overlapping': [1169, 1872, 3871], 'subsets': [1170, 1873], 'sites': [1174, 1639, 1761, 1877, 2218, 2338, 2465, 2496, 2518, 2608, 2683, 2932, 3276, 3298, 3326, 3354, 3384, 3401, 3417, 3442, 3449, 3456, 3513, 3571, 3622, 3641, 3653, 3679, 3693, 3713, 3725, 3792, 3824, 3837, 3852, 3868, 3900, 3914, 3922, 3931, 3946, 3962, 3975, 3998, 4011, 4020, 4050, 4066, 4072, 4083, 4119, 4165, 4196, 4211], 'together': [1176, 1236, 1879, 2367, 3588], 'almost': [1179, 1241, 1292, 1882], 'all': [1180, 1242, 1293, 1883, 2997, 3592, 3775, 3853], 'mRNA.': [1185, 1247, 1298, 1854, 1888], 'Finally,': [1186, 1889, 4240], 'using': [1187, 1645, 1890, 2148, 2242, 2285, 2298, 2867, 3129], 'm1A': [1188, 1891, 4293], 'an': [1190, 1218, 1893, 1955, 1980, 2090, 3799], 'example,': [1191, 1894], 'demonstrated': [1193, 1896, 2404], 'CIGAR-seq': [1195, 1208, 1826, 1898, 2062, 2073, 2146, 2194, 2365, 2590, 2628, 2798, 2838, 3075, 4229, 4247], 'can': [1196, 1302, 1899, 2048, 4263, 4275], 'be': [1197, 1303, 1723, 1795, 1900, 2049, 3053, 4199, 4231, 4276, 4301], 'easily': [1198, 1901], 'adapted': [1199, 1902], 'identifying': [1201, 1222], 'other': [1204, 1905, 3705, 3954, 4202, 4237, 4252], 'modification.': [1206, 1784, 1907, 2078, 3556], 'SYNOPSIS': [1207], 'new': [1211, 1257, 1487], 'epitranscriptomic': [1219, 1276, 1339, 1603, 1815, 1915, 1926], 'regulators.': [1225, 1266], 'methyltransferase,': [1234], 'which': [1235, 1562, 2506, 2545, 2697, 2901, 3051, 3420, 3495, 3795, 4297], 'NSUN2,': [1238, 3687], 'contributes': [1239], "'CRISPR": [1248], "sequencing'": [1253], '(CIGAR-seq)': [1254], 'The': [1267, 1687, 2079, 2191, 2255, 2402, 2466, 2515, 2537, 2579, 2911, 3181, 3199, 3487, 3507, 3778, 3849, 3869, 3904, 3938, 4018, 4067], 'study': [1268, 3404, 4177, 4235, 4250], 'presents': [1269], 'first': [1271, 1388, 1440, 1617], 'readout.': [1277], 'contribute': [1290], 'Introduction': [1299], 'RNAs': [1301], 'chemically': [1304], 'modified': [1305, 4302], 'hundred': [1309], 'different': [1310, 2561, 3949, 3977, 4167], 'ways,': [1311], 'diverse': [1319], 'conditions': [1326], '(Machnicka': [1327], 'et': [1328, 1332, 1368, 1408, 1412, 1434, 1544, 1549, 1581, 1585, 1589, 1632, 1655, 1659, 1666, 1684, 1703, 1707, 1711, 1715, 1719, 1738, 1742, 1767, 1942, 2617, 2675, 3022, 3044, 3048, 3388, 3406, 3428, 3432, 3664, 3668, 3811, 3815, 4179, 4270, 4307, 4311, 4315], 'al,': [1329, 1333, 1369, 1409, 1413, 1435, 1545, 1550, 1582, 1586, 1590, 1633, 1656, 1660, 1667, 1685, 1704, 1708, 1712, 1716, 1720, 1739, 1743, 1768, 1943, 2618, 3023, 3045, 3049, 3389, 3407, 3429, 3433, 3665, 3669, 3812, 3816, 4180, 4271, 4308, 4312, 4316], '2013;': [1330, 1736], 'Roundtree': [1331], '2017).': [1334, 1744, 4317], 'achieve': [1336], 'so-called': [1337], 'dynamic': [1338], 'regulation,': [1340], 'each': [1341, 2294, 2921], 'needs': [1343], 'its': [1344, 1502, 1960, 2095, 2824, 4273, 4323], 'distinct': [1345], 'deposition,': [1346], 'removal,': [1347], 'recognition': [1349], 'factors': [1350], '(termed': [1351], '"writers",': [1352], '"erasers",': [1353], '"readers",': [1355], 'respectively).': [1356], 'Yet,': [1357], 'comparing': [1358, 3558], 'ever-expanding': [1361], 'techniques': [1362], 'detecting': [1364], '(Zhao': [1367], '2020),': [1370], 'methods': [1372], 'systematically': [1374], 'writers': [1376], 'erasers': [1378], 'are': [1382, 3589, 3746], 'rather': [1383], 'limited.': [1384], 'For': [1385], 'instance,': [1386], 'N6-adenosine': [1389], '(m6A)': [1390], 'methyltransferase': [1391, 1422, 1728, 2069, 2597], 'METTL3': [1392, 1432], 'was': [1393, 1424, 1508, 1616, 2003, 2024, 2087, 2112, 2132, 2237, 2283, 2296, 2306, 2362, 2400, 2479, 2684, 2791, 2808, 2861, 2918, 2955, 2990, 3012, 3071, 3141, 3174, 3196, 3332, 3378, 3522, 3784, 3796, 3825, 4043, 4073, 4212], 'identified': [1394, 1618, 1846, 2063, 2498, 2591, 2685, 2991, 3299, 3457], 'through': [1395, 1426, 1837], 'combination': [1397], 'vitro': [1400], 'assay,': [1401], 'conventional': [1402], 'chromatography,': [1403], 'electrophoresis,': [1404], 'microsequencing': [1406], '(Bokar': [1407], '1994;': [1410], 'Bokar': [1411], '1997);': [1414], 'METTL14,': [1416], 'key': [1418], 'component': [1419], 'complex,': [1423], 'phylogenetic': [1428], 'analysis': [1429, 3331, 3770], 'based': [1430, 2749, 2962, 3334], '(Wang': [1433], '2014).': [1436], 'In': [1437, 1552, 1917, 2037, 3221, 3409, 3721], 'general,': [1438], 'strategy': [1441, 1453], 'less': [1443], 'efficient': [1444], 'may': [1446], 'assay-specific': [1448], 'bias,': [1449], 'while': [1450, 4037], 'second': [1452], 'relies': [1454], 'prior': [1457], 'knowledge': [1458], 'related': [1460], 'molecule(s).': [1461], 'So': [1462], 'far,': [1463], 'still': [1475], 'Recently,': [1477], 'rapid': [1479], 'development': [1480], 'CRISPR-based': [1482, 1564], 'gene': [1483, 1556, 1565, 2156, 2222, 2295, 2361, 2375, 2535, 2769, 2969, 3058, 3108], 'manipulation': [1484], 'provides': [1485], 'paradigm': [1488], 'genome-wide': [1492], 'functional': [1493], 'screening.': [1494], 'Pooled': [1495, 1910], 'outperforms': [1498], 'array-based': [1499], 'scalability': [1503], 'low': [1505, 3716, 3758, 3856, 3965, 4154], 'cost,': [1506], 'however,': [1507], 'largely': [1509, 3573, 3870], 'restricted': [1510], 'standard': [1512], 'readouts,': [1513], 'survival,': [1515], 'proliferation,': [1516], 'FACS-sortable': [1518], 'markers': [1519], '(Hanna': [1520], 'Doench,': [1522], '2020).': [1523, 1551], 'Most': [1524], 'recently,': [1525], 'microscopy-based': [1528], 'approaches,': [1529], 'enabled': [1532], 'association': [1534], 'subcellular': [1536], 'phenotypes': [1537], 'perturbation': [1539, 2408, 3039, 3087], 'specific': [1541, 2058], 'gene(s)': [1542, 2631], '(Wheeler': [1543], '2020;': [1546], 'preprint:': [1547], 'Yan': [1548], 'studying': [1553, 1904, 2076], 'regulation': [1554], 'expression,': [1557], 'Perturb-seq,': [1558], 'CRISP-seq,': [1559], 'CROP-seq,': [1561], 'combine': [1563], 'editing': [1566], 'single-cell': [1568], 'sequencing,': [1570], 'allowed': [1571], 'transcriptome': [1572], 'profile': [1573], 'serve': [1575], 'comprehensive': [1577], 'molecular': [1578], 'readout': [1579, 1604, 1916], '(Adamson': [1580], '2016;': [1583, 1587, 1705, 4309], 'Dixit': [1584], 'Datlinger': [1588], '2017),': [1591], 'but': [1592, 3756, 3773], 'often': [1593], 'limited': [1595], 'throughput.': [1596], 'Until': [1597], 'now,': [1598], 'has': [1605, 1690, 3392], 'not': [1606, 3030, 3774, 4222], 'yet': [1607], 'developed.': [1609], 'One': [1610], 'important': [1611, 1796], 'modification,': [1613, 1792], '5-methylcytosine': [1614], '(m5C),': [1615], 'stable': [1620], 'highly': [1622, 3797, 3827, 4074], 'abundant': [1623], 'tRNA': [1624, 3020], 'rRNA': [1626], '(Helm,': [1627], '2006;': [1628], 'Agris,': [1629], '2008;': [1630], 'Schaefer': [1631], '2009).': [1634], 'Subsequently,': [1635, 2013], 'many': [1636], 'were': [1642, 2205, 2497, 2527, 2747, 2834, 2851, 2893, 2933, 3211, 3355, 3572, 3604, 3633, 3642, 3654, 3695, 3923, 3932], 'next-generation': [1646, 2033, 2142, 2878], 'sequencing-based': [1647], 'methods,': [1648], '(mRNA-BisSeq)': [1653, 2661], '(Schaefer': [1654], '2009;': [1657], 'Squires': [1658], '2012),': [1661], 'm5C-RNA': [1662], 'immunoprecipitation': [1663, 1671, 1681], '(RIP)': [1664], '(Edelheit': [1665], '2013),': [1668, 1676], '5-azacytidine-mediated': [1669], '(Aza-IP)': [1672], '(Khoddami': [1673, 1733], 'Cairns,': [1675, 1735], 'methylation-individual-nucleotide-resolution': [1678], 'crosslinking': [1679], '(miCLIP)': [1682], '(Hussain': [1683], '2013).': [1686], 'reported': [1692, 3014, 3662], 'regulate': [1694], 'structure,': [1696], 'stability,': [1697], 'translation': [1699], 'mRNAs': [1701], '(Luo': [1702], '2017a;': [1709], 'Guallar': [1710], '2018;': [1713, 1717], 'Shen': [1714], 'Schumann': [1718], '2020)': [1721], 'catalyzed': [1724], 'NOP2/Sun': [1726], 'family': [1729], 'member': [1730, 2984], '2': [1731], '(NSUN2)': [1732], 'David': [1737], '2017;': [1740, 3046, 3430, 3666], 'recent': [1746], 'studies': [1747, 2602, 3028], 'shown': [1749, 2974, 3083, 3348, 3566, 3614, 3650, 4183], 'that,': [1750, 2405], 'even': [1751], 'after': [1752, 2232, 2387, 2475, 2613, 2848, 3037, 3192], '(KO),': [1755], 'significant': [1757, 3032, 3307], 'number': [1758, 2562], 'remained': [1764, 2431, 2611], 'methylated': [1765, 2612], '(Huang': [1766, 2616, 3405, 4178], '2019;': [1769, 2619], 'Trixl': [1770, 2620], 'Lusser,': [1772, 2622], '2019),': [1773, 2623, 3050], 'suggesting': [1774, 3575, 3744], 'existence': [1776], 'additional': [1778], 'methyltransferase(s)': [1779], 'involved': [1780], 'fully': [1786], 'appreciate': [1787], 'function': [1789], 'would': [1794, 4132, 4141], 'remaining': [1800, 3779, 3850], 'methyltransferase(s).': [1801], 'containing': [1830, 2420, 2436, 3077], 'site,': [1834, 1983, 3754], 'screened': [1836], 'library': [1840, 2226, 2785, 2807, 2881], 'targeting': [1841, 2056, 2167, 2227, 2379, 2422, 2786, 2966, 3069], '829': [1842, 2228, 2787], 'RNA-binding': [1843, 2788], 'proteins': [1844, 2789], 'writer': [1852], 'mRNA-BisSeq': [1855, 3611, 3769, 3991, 4172], 'Results': [1908], 'CIGAR-seq:': [1909], 'integrate': [1920], 'here': [1928, 2445, 2709, 3166, 3984, 4057], 'specifically': [1930], 'adopted': [1935], 'previously': [1937, 3013, 3661], 'developed': [1938], 'CROP-seq': [1939, 2085], '(Datlinger': [1941], '2017)': [1944], 'replaced': [1946, 2088], 'WPRE': [1948, 2080], 'cassette': [1949, 2081], 'original': [1952, 2084], 'vector': [1953, 1973, 2086, 2799, 2813, 3076], 'endogenous': [1956, 1981, 2091, 2335, 2463, 3102, 3139], 'site': [1958, 2020, 2047, 2093, 2106, 2128, 2152, 2247, 2354, 2574, 2763, 2779, 2822, 2872, 2917, 3105, 3140, 3266, 3781, 3844, 4040, 4090, 4294, 4321], 'flanking': [1961, 2017, 2096, 2125, 2244, 2825, 2869, 3323, 3343], 'region': [1962, 2029, 2137, 2827], '(Fig': [1963, 2035, 2381, 2409, 2424, 2440, 2654, 2706, 2841, 2909, 2946, 3005, 3090, 3109, 3131, 3149, 3290, 3315, 3606, 3671, 3719, 3740, 3793, 3847, 4227], '1A).': [1964, 2036], 'Thereby,': [1965], 'molecules': [1968], 'transcribed': [1969], 'from': [1970, 2154, 2220, 4174, 4295], 'lentiviral': [1972], 'contain': [1974], 'selection': [1976], 'marker': [1977], 'followed': [1978, 2009, 2117, 2814], 'U6': [1985], 'promoter': [1986], 'sequence.': [1990], 'detect': [1992], 'level': [1995, 2042, 2102, 2327, 2395, 2511, 2570, 2702, 3003, 3096, 3136, 3311, 3718, 3760], 'sequence-containing': [1999, 2109], 'transcripts,': [2000, 2110], 'total': [2001], 'firstly': [2004, 2809], 'subjected': [2005, 2113, 2854, 3609], 'treatment': [2008, 2116], 'reverse': [2011, 2119], 'transcription.': [2012, 2120], 'primer': [2015, 2123], 'pair': [2016, 2124], 'sequence': [2023, 2131, 2250, 2875, 3322, 3397, 3412, 3508, 3905], 'used': [2025, 2133, 2956, 4232], 'amplify': [2027, 2135], 'Sanger': [2031, 2140], 'way,': [2039], 'methylation': [2041], 'measured': [2050], 'gene.': [2059, 2341], 'Figure': [2060, 2450, 2714, 3171, 3435, 3859, 3989, 4062], '1.': [2061], 'An': [2070], 'illustration': [2071], 'region.': [2097], 'measure': [2099], 'Then,': [2121, 3580], 'subsequent': [2139], 'sequencing.': [2143, 4281], 'Validation': [2144, 2310], 'derived': [2153, 2219], 'FAM129B': [2155, 2179, 2356], 'control': [2159, 2188, 2370, 2438], 'target': [2162, 2374, 3383], 'genes': [2163, 2258, 2282, 2559, 2892, 2903], 'NSUN2.': [2168, 3701], 'Upon': [2169], 'knockout,': [2171], 'diminished': [2176], 'whereas': [2182, 2428], 'remains': [2185], 'intact': [2186, 2432], 'cells.': [2190, 2472, 2493, 2514, 2556, 2668, 2753, 3189, 3206, 3452, 3470, 3506, 3516, 3638, 3919], 'application': [2192, 4245], 'sites.': [2201, 2639, 3346, 3371, 3526, 3878], 'Cas9-expressing': [2202, 2390, 2470, 2831, 3187], 'transduced': [2206, 2836], 'viral': [2208, 2476, 3193], 'particles': [2209], 'express': [2211], 'Cigar': [2212], 'vectors': [2213], 'NSUN2-independent': [2215, 2336, 2638, 2644, 2762, 3103, 3400], 'FURIN': [2221, 2330, 2768, 2820, 3079], 'RBPs.': [2229], 'Seven': [2230, 2385, 2473, 2846, 3190], 'days': [2231, 2386, 2474, 2847, 3191], 'transduction,': [2233, 2477, 2849, 3194], 'enriched': [2234, 3364, 3644, 3655, 3828, 3925, 3933, 4075], 'polyA': [2235, 2859], 'bisulfite-treated,': [2238, 2863], 'reveres-transcribed,': [2239], 'PCR-amplified': [2241, 2866], 'primers': [2243, 2868], 'generate': [2252, 2877], 'NGS': [2253], 'library.': [2254], 'rank': [2256], 'whose': [2259], 'reduced': [2261, 2324, 2415, 2509, 2700, 2943, 3093, 3143, 3464], 'rate': [2264, 2414, 2523, 2548, 2775, 2914, 3262, 3477, 3498, 3885, 3940, 3969, 4161, 4208], 'site.': [2268, 3081], 'high-confident': [2272], 'candidate': [2273, 2951, 3229], 'genes,': [2274, 2952, 3230], 'information': [2275], 'multiple': [2277, 3235], 'gRNAs': [2278, 2423, 2564, 2908, 2940, 2965, 2999, 3236], 'same': [2281, 2968], 'combined': [2284, 2291, 2960], "Stouffer's": [2287, 2303, 2953], 'method,': [2288], 'then': [2289, 2759, 2835, 2862, 3542], 'P-value': [2292, 2961], 'calculated': [2297, 2919], 'weighted': [2300], 'version': [2301], 'method.': [2304], 'top': [2308], 'hit.': [2309], 'Knockout': [2318, 2453], 'well': [2320, 2461, 2665, 2735, 3689], 'knock-down': [2322], 'both': [2329, 3098, 3684, 3698, 4001], 'transcripts': [2333, 2399], 'RPSA': [2340, 3107], 'Download': [2342, 2344, 2586, 2588, 2754, 2756, 3217, 3219, 3527, 3529, 3979, 3981, 4052, 4054, 4093, 4095], 'figure': [2343, 2587, 2755, 3218, 3528, 3980, 4053, 4094], 'PowerPoint': [2345, 2589, 2757, 3220, 3530, 3982, 4055, 4096], 'As': [2346, 2598, 2973, 3082, 3347, 3613, 4182, 4260], 'proof-of-concept': [2348, 4287], 'experiment,': [2349, 4288], 'known': [2351, 2358, 4299], 'NSUN2-dependent': [2352, 3415, 3652, 3753, 3865, 3899, 3930, 3973, 4194, 4219], '(also': [2357], 'NIBAN2)': [2360], 'cloned': [2363, 2810, 4319], 'into': [2364, 2389, 2811, 3073], 'vector,': [2366], 'any': [2373], '1B,': [2382, 2425, 2441], 'upper': [2383, 2442], 'panel).': [2384, 2443, 3152], 'transduction': [2388], 'cells,': [2392, 2502, 3303, 3329, 3460, 3485], 'measured.': [2401], 'result': [2403], 'upon': [2406], 'EV1A),': [2410], 'significantly': [2416, 2508, 2699, 2942, 3463], 'lower': [2426], 'panel),': [2427, 3902], 'Click': [2444, 2708, 3165, 3983, 4056], 'expand': [2447, 2711, 3168, 3986, 4059], 'figure.': [2449, 2713, 3170, 3988, 4061], 'EV1.': [2451], 'effect': [2456, 2486, 2729, 3122, 3245], 'NSUN2-KO': [2467, 2513, 2525, 2667, 2704], 'efficiency': [2468, 3183, 3201], 'efficiently': [2480, 3175, 3197], 'mutated.': [2481, 3198], 'Scatter': [2482], 'plot': [2483, 2567], 'demonstrating': [2484, 2727], 'knocked': [2489, 3176, 3179, 3468, 3482], 'out': [2490, 2980, 3118, 3177, 3469, 3483], '208': [2494, 2681, 3296, 3454], 'wild-type': [2500, 2552, 2663, 2687, 3301, 3459, 3479, 3502], '90': [2503, 2694], '(43.3%)': [2504, 2695], 'five': [2516], 'highest': [2521, 4216], 'highlighted': [2528], 'red': [2530], 'dots': [2531], 'marked': [2533], 'names.': [2536], 'gray': [2538, 3488], 'line': [2539, 3489], 'represents': [2540, 2581, 3490], 'diagonal': [2542, 3492], 'line,': [2543, 3493], 'along': [2544, 3494], 'equal': [2550, 3500], 'between': [2551, 3501, 3559, 3862, 3873], 'Distribution': [2557], 'detected.': [2565], 'Density': [2566], 'individual': [2577], 'gRNA.': [2578, 2922], 'Y-axis': [2580, 3473], 'density': [2583], 'gRNAs.': [2585], 'suggested': [2599, 3808], 'previous': [2601, 3027, 4176, 4285], 'large': [2604, 4215], 'amount': [2605], 'sought': [2625], 'utilize': [2627], 'mediate': [2633], 'First,': [2640, 3557], 'determine': [2642], 'sites,': [2646, 3282, 3564, 4107, 4150], 'established': [2648, 2748, 3284, 3605], '(NSUN2-KO)': [2651], 'EV2A),': [2655], 'performed': [2657, 3293, 3333], 'Following': [2669], 'bioinformatic': [2670], 'pipeline': [2671], 'proposed': [2672, 3395], 'Huang': [2674, 3047, 3431, 3667, 3814], 'al': [2676], '(2019),': [2677], 'set': [2679, 4109], '(Materials': [2690, 2828, 2882, 2970, 4125], 'Methods),': [2692], 'only': [2693, 3624], 'EV1B).': [2707], 'EV2.': [2715], 'KO': [2716, 3182, 3600], 'A-C.': [2724], 'Western': [2725], 'Blot': [2726], '(A),': [2731], '(B)': [2733, 4026], 'NSUN2/6': [2737, 2741], 'double': [2738, 2742, 3599], '(C).': [2740, 4051], '(NSUN2/6-dKO)': [2744, 3601], 'clonal': [2751], 'NSUN6-KO': [2752, 3313], 'We': [2758, 3541, 4318], 'chose': [2760, 4290], "3'UTR": [2766], 'high': [2772, 4135], 'CIGAR-seq.': [2781], 'Meanwhile,': [2782], '(RBP)': [2790], 'synthesized': [2792], '(Dataset': [2793], 'EV1).': [2794], 'establish': [2796], 'pool': [2800, 2840], 'genetic': [2803], 'screen,': [2804], 'insertion': [2817], 'genomic': [2826], 'Methods).': [2830, 2845, 2884, 2972, 4127], 'virus': [2839], '1C,': [2842], 'Materials': [2843], 'collected': [2852], 'extraction.': [2857], 'Enriched': [2858], 'reveres-transcribed': [2864], '(NGS)': [2880], 'After': [2885], 'pair-end': [2886], 'data': [2889, 4173], 'processing,': [2890], '811': [2891], 'detected': [2894, 4277], 'at': [2896, 2905, 3137, 3309, 3367, 3381, 3839, 4085], 'least': [2897, 2906, 3840, 4086], 'one': [2898, 3841, 4087], 'gRNA,': [2899, 3124], '782': [2902], 'had': [2904, 3714, 3838, 3854, 4084], 'two': [2907, 3026, 3228], 'EV1C).': [2910], 'While': [2923, 3639, 3920, 4205], 'median': [2925], 'rates': [2928, 2945], 'gRNA-associated': [2930], 'around': [2934, 3399, 3414], '93.5%,': [2935], 'small': [2937], 'part': [2938], 'EV1D).': [2947], 'prioritize': [2949], 'calculate': [2958], 'Fig': [2976, 3085, 3350, 3568, 3616, 4185], '1D,': [2977], 'turned': [2979, 4256], 'NSUN6,': [2982, 3224], 'NOL1/NOP2/sun': [2986], 'domain': [2987], '(NSUN)': [2988], 'family,': [2989], 'best': [2994], 'hit': [2995], 'six': [2998], 'decreasing': [3000], 'effectively': [3004], '1D': [3006], 'Dataset': [3008, 3241], 'EV2).': [3009], 'Interestingly,': [3010], 'introduce': [3016], '(Li': [3021, 3387], '2019).': [3024, 3408, 3434, 3817, 4181], 'did': [3029, 4221], 'show': [3031, 4223], 'changes': [3034], 'HeLa': [3041], '(Yang': [3043, 3427, 3663], 'might': [3052], 'due': [3054, 3801, 4120], 'incomplete': [3057, 3804, 4122], 'silencing': [3059], 'mediated': [3060], 'siRNA.': [3062], 'validate': [3064], 'our': [3065, 4138], 'result,': [3066], 'inserted': [3072], '1E,': [3086, 3110, 3150], 'EV3A)': [3091], 'indeed': [3092], 'left': [3111], 'middle': [3113], 'panels).': [3114], 'Furthermore,': [3115, 3673], 'rule': [3117], 'potential': [3120, 4114, 4244], 'off-target': [3121], 'repressed': [3126], 'expression': [3128], 'shRNA': [3130], 'EV3B).': [3132], 'Again,': [3133], 'also': [3142, 3379, 3393, 3634, 3826, 4143, 4198], 'repression': [3148], 'right': [3151], 'Together,': [3153], 'results': [3155], 'confirmed': [3156], 'bona': [3160], 'fide': [3161], 'EV3.': [3172], 'down': [3180], 'knockdown': [3200], 'Three': [3207], 'independent': [3208, 3696], 'replicates': [3210], 'performed.': [3212], 'Error': [3213], 'bars': [3214], 'represent': [3215], 'SD.': [3216], 'addition': [3222], 'selected': [3226], 'another': [3227, 3403], 'EIF3J': [3231, 3257], 'ZCCHC11': [3233, 3255], '(five': [3237], 'six,': [3239], 'respectively,': [3240, 3755, 4036], 'EV2)': [3242], 'showing': [3243, 3881, 4008], 'inhibitory': [3244], 'validation.': [3250], 'neither': [3254], 'nor': [3256], 'could': [3258, 4197, 4230], 'reduce': [3259], 'transcripts.': [3270], 'Global': [3271, 3437], 'profiling': [3272, 3438], 'NSUN6-dependent': [3274, 3280, 3324, 3352, 3440, 3447, 3511, 3525, 3562, 3640, 3876, 3921, 4210], 'globally': [3278], 'characterize': [3279, 4101], '(NSUN6-KO)': [3287], 'EV2B)': [3291], 'mRNA-BisSeq.': [3294], 'Of': [3295, 3453, 4097], '65': [3304, 3461], '(31.2%)': [3305], 'reduction': [3308], '2A).': [3316], 'illustrate': [3318], 'features': [3320, 3509, 3906], 'motif': [3330, 3366, 3377, 3398, 3426, 3521, 3648, 3659], 'upstream': [3337], 'downstream': [3339], '10': [3340], 'nucleotide': [3341], 'sequences': [3342], '2B,': [3351], 'embedded': [3356, 3785], 'slightly': [3358], 'GC-rich': [3359], 'environments': [3360], 'strongly': [3363, 3643, 3924], 'TCCA': [3365, 3376, 3520, 3647, 3733, 3928, 4030], '3′': [3368, 3375, 3424, 3519, 3646, 3657, 3732, 3736, 3927, 3935, 4029, 4033], 'Previously,': [3372], 'similar': [3374], 'found': [3380, 3523], 'tRNAs': [3386], '2019)': [3390, 3670], 'comparison,': [3410], 'feature': [3413], 'distinct,': [3419], 'enrich': [3422], 'NGGG': [3425, 3658, 3936], '2.': [3436], 'revealed': [3446], 'X-': [3471], 'represented': [3474], 'respectively.': [3486, 3903], 'A': [3517], 'strong': [3518], 'Contribution': [3531], 'evaluated': [3543], 'relative': [3545], 'contribution': [3546], 'global': [3553], 'NSUN2-': [3560, 3874], '3A,': [3569], 'non-overlapping,': [3574], 'their': [3576], 'non-redundant': [3577], 'functions.': [3579], 'further': [3582], 'examine': [3583], 'whether': [3584], 'responsible': [3590], 'EV2C)': [3607], 'analysis.': [3612], '3B,': [3617], 'depend': [3623, 3999], '(62': [3629], '87,': [3631], 'respectively)': [3632], 'abolished': [3635], 'NSUN2/6-dKO': [3637, 3891], 'earlier,': [3651], '3C).': [3672], 'carefully': [3675], 'examined': [3676], 'three': [3678, 3706, 3711, 3955, 3960, 3995], 'dependence': [3682], '56': [3692, 3822, 4070], 'Comparing': [3702, 3951], 'groups,': [3707, 3956], 'group': [3709, 3820, 3958, 4068], 'overlapping': [3712, 3961], 'very': [3715, 3747, 3855, 3964], '3D).': [3720], 'addition,': [3722], 'ANGEL1': [3727, 4022], 'ZNF707': [3729, 4025], 'possessed': [3730, 4027], 'AGGG': [3737, 4034], 'motif,': [3738, 3929, 4035], 'respectively': [3739], 'EV4A': [3741], 'B),': [3743], 'they': [3745], 'likely': [3748, 3798, 4142], 'NSUN6-': [3750, 3863, 3895, 3971, 4103, 4191], 'led': [3762], 'false': [3764, 4115], 'negative': [3765], 'findings': [3766], 'some': [3772, 4145], 'samples.': [3777], 'STRN4': [3783, 4016, 4042], 'within': [3786], 'cluster': [3788, 4046], '"pseudo"': [3790, 4048], 'EV4C),': [3794], 'artifact': [3800], 'conversion': [3806, 4124], 'before': [3809], '(Haag': [3810], '2015;': [3813], 'Similarly,': [3818], 'NSUN2/6-independent': [3823, 4064, 4071], 'clusters': [3831, 4077], 'pseudo': [3833, 3842, 4079, 4088], 'sites:': [3835, 4081], '52': [3836, 4082], 'vicinity': [3846], 'EV5).': [3848], 'four': [3851], 'level.': [3858, 3967], '3.': [3860], 'Comparison': [3861], 'pattern': [3872], 'Heat': [3879], 'map': [3880], 'wild-type,': [3887], 'NSUN2-KO,': [3888], 'NSUN6-KO,': [3889], '(upper': [3896, 3909], 'panel)': [3897, 3910, 3916], '(lower': [3901, 3915], 'NSUN6-only-': [3908], 'NSUN2-only-dependent': [3912], 'motif.': [3937], '4': [3942], 'groups': [3943], 'dependence.': [3950], 'Modification': [3968], 'across': [3976, 4166], 'tissues.': [3978], 'EV4.': [3990], 'profiles': [3992], 'A–C.': [4005], 'IGV': [4006], 'plots': [4007], 'ANGEL1,': [4013], 'ZNF707,': [4014], 'genes.': [4017], '(A)': [4023], 'among': [4044], 'EV5.': [4063], 'vicinity.': [4092], 'note,': [4098], 'here,': [4099], 'NSUN2-in/dependent': [4105], 'stringent': [4110, 4130], 'criteria': [4111, 4131], 'avoid': [4113], 'discovery': [4116], 'Whereas': [4128], 'assure': [4133], 'specificity': [4136], 'findings,': [4139], 'miss': [4144], 'true': [4148], 'particularly': [4151], 'those': [4152], 'explore': [4158, 4242], 'NSUN6/2-dependent': [4163], 'tissues,': [4168], 'resorted': [4170], '3E,': [4186], '47': [4190], '66': [4193], 'observed': [4200], 'human': [4203], 'tissue(s).': [4204], 'liver,': [4218], 'ones': [4220], 'tissue': [4225], 'biases': [4226], '3E).': [4228], 'N-1-methyladenosine': [4258, 4261], '(m1A).': [4259], '(m1A)': [4262], 'cause': [4264], 'mis-incorporation': [4265], 'during': [4266], 'cDNA': [4267, 4280], 'synthesis': [4268], '(Hauenschild': [4269], '2015),': [4272], 'direct': [4279], 'Similar': [4282], 'well-characterized': [4292], 'MALAT1,': [4296], 'TRMT6/TRMT61A': [4304], 'complex': [4305], '(Dominissini': [4306], '2017b;': [4313], 'Safra': [4314]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W3107817913', 'counts_by_year': [{'year': 2024, 'cited_by_count': 10}, {'year': 2023, 'cited_by_count': 8}, {'year': 2022, 'cited_by_count': 6}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 1}], 'updated_date': '2025-01-09T08:24:53.038179', 'created_date': '2020-12-07'}