Title: EXPRESSION OF MARKERS OF APOPTOSIS (P53, BCL-2, BAX) AND PROLIFERATION (KI-67, TOPOISOMERASE IIA, ARGYROPHILIC PROTEINS OF NUCLEOLAR ORGANIZER REGIONS) IN HEALTHY PERSONS AND NON-SMALL CELL LUNG CANCER PATIENTS AND THEIR RELATIONSHIP WITH SOME CLINICAL AND MORPHOLOGICAL PARAMETERS
Abstract: Study of markers of apoptosis and proliferation in relation to clinical and morphological parameters in non-small cell cancer (NSCLC) patients and healthy persons. There were investigated 237 surgery samples of NSCLC and 10 cases of normal lung tissues. p53, bcl-2, bax, Ki-67, topoisomerase IIα (ТороIIα) levels were determined by immunohistochemical method. Argyrophilic proteins associated with nucleolar organizer regions (AgNOR) were detected with silver nitrate. In NSCLC expression of p53, bcl-2, Ki-67, TopoIIα, AgNOR increased and expression of bax was lower if compared with the normal tissue. Expression of apoptotic markers (p53, bcl-2, bax) correlated with clinical and morphological parameters of NSCLC: histogenesis, N index and the stage (for bcl-2), gender (for bax). Expression of Ki-67 and TopoIIα correlated with gender, largest size of the tumor, histogenesis and differentiation. AgNOR proteins were interrelated with the gender, T and N indices, largest tumor dimension, stage, histogenesis and differentiation. In NSCLC cases the expression of apoptotic markers correlated with proliferation: in p53 positive tumors - high level of proliferation (Ki-67, TopoIIα, AgNOR), in bcl-2 positive tumors - low AgNOR, in bax positive tumors - low proliferative activity (Ki-67, TopoIIα). In NSCLC there is observed the change in expression of apoptotic markers (p53, bcl-2, bax) and proliferation (Ki-67, TopoIIα, AgNOR) in conjunction with normal tissue and clinical and morphological parameters of tumor.