Title: Dark fermentative biohydrogen production from rice mill wastewater
Abstract: International Journal of Energy ResearchVolume 45, Issue 12 p. 17233-17243 SPECIAL ISSUE RESEARCH ARTICLE Dark fermentative biohydrogen production from rice mill wastewater Satheesh Murugan Ramu, Satheesh Murugan Ramu Department of Energy Science, Alagappa University, Karaikudi, India Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorGujuluva Hari Dinesh, Gujuluva Hari Dinesh Department of Energy Science, Alagappa University, Karaikudi, India Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorBoobalan Thulasinathan, Boobalan Thulasinathan Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorAngelin Swetha Thondi Rajan, Angelin Swetha Thondi Rajan Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorKumar Ponnuchamy, Kumar Ponnuchamy Department of Animal Health and Management, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorArivalagan Pugazhendhi, Arivalagan Pugazhendhi orcid.org/0000-0002-9529-3306 Institute of Research and Development, Duy Tan University, Da Nang, VietnamSearch for more papers by this authorArun Alagarsamy, Corresponding Author Arun Alagarsamy [email protected] orcid.org/0000-0002-2804-9450 Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, India Correspondence Arun Alagarsamy, Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi 630003, Tamil Nadu, India. Email: [email protected]Search for more papers by this author Satheesh Murugan Ramu, Satheesh Murugan Ramu Department of Energy Science, Alagappa University, Karaikudi, India Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorGujuluva Hari Dinesh, Gujuluva Hari Dinesh Department of Energy Science, Alagappa University, Karaikudi, India Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorBoobalan Thulasinathan, Boobalan Thulasinathan Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorAngelin Swetha Thondi Rajan, Angelin Swetha Thondi Rajan Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorKumar Ponnuchamy, Kumar Ponnuchamy Department of Animal Health and Management, Alagappa University, Karaikudi, IndiaSearch for more papers by this authorArivalagan Pugazhendhi, Arivalagan Pugazhendhi orcid.org/0000-0002-9529-3306 Institute of Research and Development, Duy Tan University, Da Nang, VietnamSearch for more papers by this authorArun Alagarsamy, Corresponding Author Arun Alagarsamy [email protected] orcid.org/0000-0002-2804-9450 Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi, India Correspondence Arun Alagarsamy, Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi 630003, Tamil Nadu, India. Email: [email protected]Search for more papers by this author First published: 27 August 2020 https://doi.org/10.1002/er.5829Citations: 8 Satheesh Murugan Ramu and Gujuluva Hari Dinesh have equally contributed to this work. Funding information: Alagappa University; University Research and Scientific Excellence; Science and Engineering Research Board Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary Hydrogen (H2) is one of the most promising fuels for sustainable energy production. Microbial bio-H2 fermentation is considered to be one of the environment friendly alternatives for satisfying the demand for fossil fuels. The study has aimed to isolate the effective bio-H2 producing microbes from rice mill wastewater (RMWW) using various pre-treatment enrichment methods. Among the 27 enriched isolates, three isolates produced higher H2 utilizing rice mill wastewater as the substrate. Molecular level identification revealed that the three bacterial strains were Bacillus thuringiensis-RH1, Bacillus aerophilus-RH2, and Bacillus beringensis-AARAT1. Optimization studies revealed that B. thuringiensis-RH1 would be a promising bio-H2 producing bacterium with a yield of 0.96 ± 0.06 mol H2/ mol glucose utilizing 50% of rice mill wastewater at pH 5.5. Furthermore, the obtained optimized substrate concentration (50%) and pH (5.5) from batch fermentation were used in the 3 L batch bioreactor experiment using B. thuringiensis-RH1. A higher hydrogen yield (HY) of 1.63 ± 0.14 mol H2/mol glucose using B. thuringiensis-RH1 at 72 hours was obtained in the extensive scale up process. Citing Literature Volume45, Issue12Special Issue: Environmentally Friendly Energy Solutions10 October 2021Pages 17233-17243 RelatedInformation