Title: Expression and significance of vascular endothelial growth factor receptor 3 and PI3K/Akt in gastric carcinoma tissue.
Abstract: Objective To examine the expression of the vascular endothelial growth factor receptor 3,phosphatidylinositol-3-kinase(PI3K) and protein kinase B ( PKB or Akt) in gastric carcinoma and their adjacent normal tissues.Methods The mRNA expressions of VEGFR-3,PI3K and Akt in 36 cases of gastric carcinoma and their adjacent normal tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR).The protein expressions of VEGFR-3,P-Akt in the same tissues were detected by immunohistochemical method.Results 1.RT-PCR the mRNA expression levels of VEGFR-3,PI3K and Akt in gastric carcinoma tissues were higher than those of the adjacent normal tissues (P0.05).VEGFR-3 mRNA expression was correlated with PI3K mRNA and Akt mRNA expression(P0.05).The mRNA expression level of VEGFR-3 was related TNM stage,depth of invasion,lymphnode metastasis and distant metastasis (P0.05).2.Immunohistochemical the protein expression levels of VEGFR-3 and P-Akt in gastric carcinoma tissues were higher than those of the adjacent normal tissues (P0.05).VEGFR-3 protein expression was correlated with P-Akt protein expression (P0.05).Conclusion The tumors with VEGFR-3 expression are more likely to have lymphnode metastasis VEGFR-3 is an important predictor for the prognosis of gastric carcinoma.The expression of VEGFR-3 and PI3K/Akt have some relations.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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