Title: Cohesin in space and time: architecture and oligomerization<i>in vivo</i>
Abstract: Abstract Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair and transcription regulation. Cohesin can tether two regions of DNA and can also extrude DNA loops. We interrogated cohesin architecture, oligomerization state and function of cohesin oligomers in vivo through proximity-dependent labeling of cohesin domains. Our results suggest that the hinge and head domains of cohesin both bind DNA, and that cohesin coiled coils bend, bringing the head and hinge together to form a butterfly conformation. Our data also suggest that cohesin efficiently oligomerizes on and off DNA. The levels of oligomers and their distribution on chromosomes are cell cycle regulated. Cohesin oligomerization is blocked by mutations in distinct domains of Smc3p and Mcd1p, or depletion of Pds5p. This unusual subset of mutations specifically blocks the maintenance of cohesion and condensation, suggesting that cohesin oligomerization plays a critical role in these biological functions.