Title: The supplementation of dietary selenium yeast and green tea‐derived polyphenols improves antioxidant capacity and immune response in juvenile Wuchang bream under ammonia stress
Abstract: Aquaculture ResearchVolume 51, Issue 9 p. 3790-3803 ORIGINAL ARTICLE The supplementation of dietary selenium yeast and green tea-derived polyphenols improves antioxidant capacity and immune response in juvenile Wuchang bream under ammonia stress Honghui Guo, Honghui Guo College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorWang Lin, Wang Lin College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLingkai Wang, Lingkai Wang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorDandan Zhang, Dandan Zhang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorXueyang Wu, Xueyang Wu College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLi Li, Corresponding Author Li Li [email protected] orcid.org/0000-0002-2243-4614 College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, China Correspondence Li Li, Fisheries College of Huazhong Agricultural University, No.1 Shizishan Street, Wuhan 430070, China. Email: [email protected] for more papers by this authorDapeng Li, Dapeng Li College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorRong Tang, Rong Tang College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLiping Yang, Liping Yang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorYuming Qiu, Yuming Qiu College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this author Honghui Guo, Honghui Guo College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorWang Lin, Wang Lin College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLingkai Wang, Lingkai Wang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorDandan Zhang, Dandan Zhang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorXueyang Wu, Xueyang Wu College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLi Li, Corresponding Author Li Li [email protected] orcid.org/0000-0002-2243-4614 College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, China Correspondence Li Li, Fisheries College of Huazhong Agricultural University, No.1 Shizishan Street, Wuhan 430070, China. Email: [email protected] for more papers by this authorDapeng Li, Dapeng Li College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorRong Tang, Rong Tang College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, China National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorLiping Yang, Liping Yang College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this authorYuming Qiu, Yuming Qiu College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaSearch for more papers by this author First published: 09 July 2020 https://doi.org/10.1111/are.14724Citations: 4Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract In order to investigate protective effects of dietary selenium yeast and green tea-derived polyphenols on ammonia stress, healthy Wuchang bream juveniles (3.24 ± 0.08 g) were fed basal diets and three treatment diets supplemented with 0.50 mg/kg selenium yeast, 50 mg/kg green tea-derived polyphenols, and combination of 0.50 mg/kg selenium yeast and 50 mg/kg green tea-derived polyphenols, respectively. After a 60-day feeding, fish were exposed to 22.5 mg/L total ammonia nitrogen for 48 hr. The results showed that total antioxidant capacity, catalase and glutathione peroxidase in the serum were higher in three treatment groups than in control group before and after ammonia exposure (p < .05), while serum cortisol was lower in three treatment groups (p < .05). Similarly, the content and mRNA levels of splenic interleukin 1β, tumour necrosis factor α and immunoglobulin M showed significant increases in three treatment groups in relative to the controls (p < .05). Splenic lesions for three treatment groups were less severe, and the healing process of the lesions was faster than the control group after ammonia exposure. These findings demonstrated that single and combined supplementation of selenium yeast and green tea-derived polyphenols could enhance antioxidant and immune responses to alleviate oxidative stress and damage by ammonia. Overall, the combination of selenium yeast and green tea-derived polyphenols has a potential use as a preventive or therapeutic measure in ammonia-exposed fish. Citing Literature Supporting Information Filename Description are14724-sup-0001-TableS1.docWord document, 27.5 KB Table S1 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume51, Issue9September 2020Pages 3790-3803 RelatedInformation