Title: Construction of IRXI eukaryotic expression vector and its sub-cellular localization in SGC-7901 gastric cancer cell line
Abstract: Objective To construct the eukaryotic expression vector of pEGFP-IRX1, and observe the IRX1 protein expression and its sub-cellular localization in SGC-7901 gastric cancer cell line. Methods The coding sequence of IRX1 1443 bp was obtained by PCR amplification,then the PCR product was in-serted into the pGEM-Teasy vector. After confirming the DNA sequence, IRX1 amplification product was sub-cloned into pEGFP-N1 vector. Finally, an IRX1 gene eukaryotic expression vector was obtained. The pEGFP-IRX1 expression vector was transfected into SGC-7901 gastric cancer cell line by Lipofectamine 2000,and the expression of pEGFP-IRX1 was detected by GFP fluorescence. Results The recombinant vector was highly expressed in SGC-7901 cells. The green fluorescence of EGFP-1RX1 fusion protein was clearly localized in nuclei of gastric cancer cells. Conclusion pEGFP-IRX1 expression vector was con-structed successfully, and the IRX1 protein was mainly observed in nuclei,which settled a basis for further functional study of gene IRX1 in gastric cancer.
Key words:
Gastric carcinoma; IRX1; Eukaryotic expression vector
Publication Year: 2009
Publication Date: 2009-07-08
Language: en
Type: article
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