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['https://openalex.org/W3146095665', 'https://openalex.org/W3143342038', 'https://openalex.org/W3029272288', 'https://openalex.org/W3005430370', 'https://openalex.org/W2919706334', 'https://openalex.org/W24785758', 'https://openalex.org/W2443028281', 'https://openalex.org/W2394425474', 'https://openalex.org/W2388843995', 'https://openalex.org/W2386595679', 'https://openalex.org/W2383858297', 'https://openalex.org/W2382246306', 'https://openalex.org/W2380026878', 'https://openalex.org/W2379614574', 'https://openalex.org/W2374178088', 'https://openalex.org/W2373646280', 'https://openalex.org/W2367852305', 'https://openalex.org/W2358503335', 'https://openalex.org/W2349278031', 'https://openalex.org/W188180324'], 'abstract_inverted_index': {'目的': [0], '构建编码人CD80基因的真核表达载体,转染神经胶质瘤细胞株U251,并检测CD80基因在U251中的表达.方法': [1], 'PCR法扩增人类CD80基因的开放阅读框(ORF)全长,酶切法连接入真核表达载体pcDNA3.1,构建为重组质粒pcDNA3.1/CD80,双酶切及测序鉴定.利用脂质体法将pcDNA3.1/CD80转染U251细胞株,应用RT-PCR、流式细胞术、免疫细胞化学法、Western': [2], 'blotting等技术从细胞及分子水平检测人CD80分子在U251细胞表面的表达情况.结果': [3], '重组质粒pcDNA3.1/CD80双酶切可切出约900bp目的': [4], '片段,测序结果和NCBI检索CD80序列吻合;RT-PCR检测到pcDNA3.1/CD80转染后的U251细胞中CD80': [5], 'mRNA表达;荧光显微镜下观察可见大量免疫荧光标记的绿色荧光细胞,检测转染效率约31.8%;Western': [6], 'blotting可检测到约60': [7], 'kD蛋白条带.结论': [8], '本实验成功构建了pcDNA3.1/CD80真核表达载体,并实现了在神经胶质瘤细胞株U251中的表达,为后续研究CD80的表达对肿瘤细胞免疫原性的影响以及制备神经胶质瘤肿瘤疫苗提供了重要的实验材料。': [9]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W3032769479', 'counts_by_year': [], 'updated_date': '2024-09-18T10:04:07.804818', 'created_date': '2020-06-05'}