Title: Effects of sodium aescinate on proliferation and apoptosis of human cholandiocarcinoma Hccc9810 cell line
Abstract: Objective To investigate the effects of sodium aescinate on the proliferation and apoptosis of cholangiocarcinoma Hccc9810 cell line.Methods The effects of sodium aescinate (0,10,20,40μmol/L) on cell growth,cycle and apoptosis were investigated by methyl thiazol tetrazolium (MTT) assay,flow cytometry,and fluorescence microscopy.Results Sodium aescinate inhibited cholangiocarcinoma cell growth in a dose- and time-dependent manner,and its half-inhibitory concentration (IC50) for 24 h,48 h,and 72 h was (45.34 ±2.12),(33.00± 1.92) and (25.00 ± 1.65) μmol/L respectively.The proportion of cells in G1 phase was increased from (49.49 ± 1.22)% to (70.20 ± 1.52)%.The apoptosis rote was increased from 20.54% to 62.56% (P <0.05).Conclusion Sodium aescinate exhibited its anticarcinoma effect probably through regulating cell cycle and inducing cell apoptosis.
Key words:
Sodium aescinate; Proliferation; Cholangiocarcinoma
Publication Year: 2011
Publication Date: 2011-11-08
Language: en
Type: article
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