Title: Propofol attenuates c-Jun terninal kinase activation in lipopolgsaccharides-induced acute lung injury in rats
Abstract: Objective To investigate the effects of c-Jun terninal kinase(JNK)activation post-trested by propofol on lipopolysaccharide-induced acute lung injury.Methods Ninety six male SD rats were randomly divided into 4 equal groups(n=24):the control group(0.9% sodium chloride group); Lipopolysaccharides(LPS)group(intravenous injection of LPS 5 mg/kg); low dose of propofol group(2 mg/kg induced and 4 mg·kg1·h-1 maintained); large dose of propofol group(4 mg/kg induced and 8 mg·kg-1 ·h-1 maintained).Six rats were killed at each time point after LPS intravenous administration(saline in group A)and the lungs were harved and the activation of Phospho c-Jun terninal kinase(p-JNK)was recorded in the lung tissues by the method of western-blot and Immunocytoche-mical(IHC).Results Western-blot and IHC shown that the activation of JNK in the lung tissues increased significantly after LPS administration(P=0.02-0.03<0.05 or P=0.006-0.008<0.01).The number ofp-JNK was 322±32 and 21.7±4.4 shown by western-blot and IHC at the time point of 3 h after LPS administration.The p-JNK at all time points declined significantly after treatment with propofol in group C and group D and the p-JNK declined to 235±26 and 179±21(western-blot)and 15.1±3.1 and 12.3±1.7(IHC)at the time point of 3 h.Compared with group C,p-JNK declined more than those in group D at the time point of 3 h after after LPS administration.Conclusion Treatment with propofol can significantly attenuate the p-JNK expression in LPS-induced acute lung injury in rats.
Key words:
Propofol; Endotoxin; Acute lung injury; C-Jun terninal kinase
Publication Year: 2011
Publication Date: 2011-10-15
Language: en
Type: article
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