Title: The expression of annexin A10 in hepatocellular carcinoma and its correlation with matrix metalloproteinase 9 and vascular endothelial growth factor
Abstract: Objective
To explore the expression of annexin A10 (ANXA10) in human hepatocellular carcinoma (HCC), and analyze its correlation with matrix metalloproteinases-9 (MMP-9) and vascular endothelial growth factor (VEGF), thus to provide the basis for clinical diagnosis and assessment of HCC.
Methods
88 HCC patients were selected, and they were all performed surgical treatment, HCC stage Ⅰin 11 cases, stageⅡ 25 cases, stage Ⅲ 31 cases, stage Ⅳ 21 cases.The expressions of ANXA10, MMP-9, VEGF of HCC cancer tissues, adjacent liver tissues and normal liver tissues were tested by immunohistochemical method.The correlation of ANXA10 with VEGF and MMP-9 was analyzed.
Results
The absorbance value of ANXA10 expression in the HCC cancer tissue was (0.074±0.012), which was lower than that in the adjacent liver tissues[(0.091±0.013)] and normal liver tissues[(0.131±0.025)], and ANXA10 expression of the adjacent liver tissue was lower than that of the normal liver tissues, the differences were statistically significant (t=8.96, 9.44, 8.71, all P<0.05). The absorbance values of MMP-9 and VEGF expression in the HCC cancer tissue were (0.147±0.017) and (0.127±0.028), respectively, which were higher than those in the adjacent liver tissues [(0.096±0.012), (0.091±0.015)] and normal liver tissues [(0.075±0.014), (0.077±0.019)]. The absorbance values of MMP-9 and VEGF of the adjacent liver tissues were higher than those of the normal liver tissues, the differences were statistically significant (t=8.05, 9.30, 8.11; 8.28, 9.51, 8.02, all P<0.05). The absorbance value of ANXA10 expression in the HCC stage Ⅲ+ Ⅳcancer tissue was (0.056±0.010), which was lower than that in the stage Ⅰ+ Ⅱcancer tissue [(0.082±0.016)], the difference was statistically significant (t=8.90, P<0.05). The absorbance values of MMP-9 and VEGF expression in the stage Ⅲ+ Ⅳcancer tissue were (0.157±0.022) and (0.169±0.033), respectively, which were higher than those in the stage Ⅰ+ Ⅱ cancer tissue [(0.114±0.015), (0.091±0.021)], the differences were statistically significant (t=9.13, 9.72, all P<0.05). ANXA10 was correlated with MMP-9 and VEGF (r=0.324, 0.295, all P<0.05).
Conclusion
ANXA10 presents lower expression in HCC cell, and the expression decreased with the increase of staging.It is negatively related with the MMP-9 and VEGF.ANXA10 expression missing or inactivation of malignant change may be one of the most important features in HCC.
Key words:
Carcinoma, hepatocellular; Annexins; Matrix metalloproteinase 9; Vascular endothelial growth factors
Publication Year: 2017
Publication Date: 2017-10-15
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot