Title: Transplantation of PKH26-1abeled bone mesenchymal stem cells via intercostal arteries to promote neurological function recovery in rabbits with spinal cord injury
Abstract: Objective To investigate the effect of transplantation of PKH26-1abeled bone mesenchymal stem cells (BMSCs) via intercostal arteries on the neurological function recovery in rabbits with spinal cord injury (SCI).Methods The SCI models were made by aneurysm clips at the level of T9 segment in 20 rabbits.The successful rabbit SCI models were randomly divided into 2 equal groups.Seven days after SCI,the control group was not treated while in the transplantation group 0.5 mL of PKH26-1abeled BMSCs was injected through intercostal arteries.Basso-Beattie-Bresnahan (BBB) scoring and neuro-electrophysiology detection were conducted before and after SCI.The animals were sacrificed to harvest spinal cord specimens for histopathological examination 21 days after SCI.Results There were no significant differences between the 2 groups in BBB score 7 days after SCI (P > 0.05).At 14 and 21 days after SCI,the BBB scores were significantly higher and recovery of motor evoked potentials (MEP) and somatosensory evoked potentials (SEP) was significantly better in the transplantation group than in the control group (P < 0.05).PKH26-positive BMSCs gathered at the injured spinal sites 21 days after SCI in the transplantation group.Hematoxylin-Eosin (HE) staining showed visible glial scar tissue and cavity formation at the injured spinal sites in the 2 groups 21 days after SCI,but the cavity area in the transplantation group was smaller than in the control group.NF 200 positive expression was significantly enhanced in the transplantation group than in the control group (P < 0.05).Conclusion Transplantation of PKH26-1abeled BMSCs via intercostal arteries can effectively promote the neurological function recovery in rabbits with spinal cord injury.
Key words:
Bone marrow; Mesenchymal stem cells; Spinal cord injury; Intercostal arteries; PKH26
Publication Year: 2014
Publication Date: 2014-03-15
Language: en
Type: article
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