Title: Effect of astilbin on expression of supressors of cytokine signaling 1 in liver warm ischemia-reperfusion injury
Abstract: Objective To study the molecular mechanism of the effect of astilbin on the anti-inflammation signaling pathway in liver warm ischemia-reperfusion injury.Methods C57BL/6 mice were randomly divided into four groups (n =5):sham-operated group (Sham),model control group (I/R),low dosage of astilbin treatment group (10 mg/kg) and high dosage of astilbin (40 mg/kg) treatment group.Twenty-four h and one h before Ischemia,the mice in the treatment groups were intraperitoneally injected with 10 or 40 mg/kg astilbin.Then the hepatic ischemia-reperfusion model of 70 percent of liver,including the left and middle hepatic lobes,was established.The I/R model control group and the shamoperated group were administered with the same volume of normal saline.After 90 min ischemia and 6 h reperfusion of the partial hepatic lobe,liver tissue samples were collected from the experimental groups.The contents of supressors of cytokine signaling 1 (SOCS-1) and interleukin (IL)-10 in liver tissues were detected by using Western blotting.The mRNA expression of the same proteins was detected by using semiquantitative reverse transcriptase-polymerase chain reaction (SqRT-PCR).Results As compared with the I/R model control group,SOCS-1 and IL-10 protein levels were gradually increased in treatment groups,and higher in the high dosage group than in the low dosage group.The result of mRNA expression showed a same trend (P < 0.05 for low dosage group; P < 0.01 for high dosage group).Conclusion Intervention with astilbin can promote the expression of IL-10 protein and mRNA in IRI liver,thus inhibiting the inflammation; Intervention with astilbin can promote the expression of SOCS-1 protein and mRNA in IRI liver,showing its role of negative regulation by upregulating SOCS-1 pathway.
Key words:
Astilbin; Liver ischemia; Reperfusion injury; Interleukin-10; Supressors of cytokine signaling 1
Publication Year: 2014
Publication Date: 2014-04-08
Language: en
Type: article
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