Title: Effect of antisense K-ras gene on proliferation and apoptosis of pancreatic carcinoma cells
Abstract: Objective To evaluate the effect of antisense gene targeted to the oncogene K-ras on pancreatic carcinoma cell proliferation, apoptosis and expression of Fas/FasL, Bcl-2 and Bax genes.Methods The recombinant retroviral vector pLXSN was transfected into packaging cell line PT-67.Human pancreatic carcinoma cell lines BxPC-3 and PC-3 were infected with the recombinant retrovirus and followed by selection with G418. Cell proliferation,apoptosis and the expression of related genes were assessed by MTT,flow cytometry and immunohistochemistry (IHC). Results The recombinant retrovirus encoding antisense K-ras gene was constructed successfully. The proliferation of PC-3 cells and the transplanted tumor in nude mice (with the point mutation GGT to GTT at codon 12 of K-ras gene) were inhibited,the proportion of G0/1 phase cells increased and the S phase cells decreased signif-icantly while the apoptosis of cells increased. Meanwhile,the increase of Bax/Bcl-2 ratio and the up-regulation of Fas expression of retroviruses infected PC-3 were detected by IHC assay. Conclusion The antisense K-ras gene can inhibit the proliferation of human pancreatic carcinoma cells linetransplanted tumors in nude mice and induce apoptosis by up-regulating the ratio of Bcl-2/Bax and/or promoting the expression of Fas.
Key words:
Pancreatic neoplasms; Cell apoptosis; Gene therapy
Publication Year: 2008
Publication Date: 2008-07-28
Language: en
Type: article
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