Title: Effect of a-lipoic acid on cerebal injury induced by cardiopulmonary bypass in dogs
Abstract: Objective To investigate the effect of a-lipoic acid on cerebral injury induced by cardiopulmonary bypass (CPB) in dogs. Methods Twelve adult healthy mengrel dogs of both sexes weighing 13.5-17.5 kg were randomly divided into control group (group C) and a-lopoic acid group (group L) (n = 6 each). In group L a-lipoic acid 50 mg/kg was injected iv immediately before CPB. The animals were anesthetized with intraperitoneal 2.5 % pentobarbital 25 mg/kg, intubated and mechanically ventilated. PaCO2 was maintained at 35-40 mm Hg. Femoral artery and vein were cannulated and Swan-Ganz catheter was inserted into pulmonary artery for MAP, CVP and CO monitoring and blood sampling. Blood samples were obtained immediately before aortic cross-clamping (T0 , baseline), at 30 and 60 min after aortic cross-clamping (T1,2) and 30 and 60 min after aortic unclamping (T3,4 ) for measurement of plasma concentrations of TNF-a, S100β protein and neuron-specific enolase (NSE). Results In group C CPB significantly increased plasma TNF-a, S100β protein and NSE concentrations as compared with the baseline values at TO . Pretreatment with a-lipoic acid significantly attenuated CPB-induced increase in plasma TNF-a, S100β protein and NSE concentrations in group L. Plasma S100β protein and NSE levels were positively correlated with plasma TNF-a level. Conclusion Pretreatment with a-lipoic acid is effective in attenuating CPB-induced inflammatory response and cerebral injury.
Key words:
Antioxidants ; Cardiopulmonary bypass ; Brain injuries
Publication Year: 2010
Publication Date: 2010-07-20
Language: en
Type: article
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