Title: Establishment and validation of direct immunofluorescence method to detect rabies virus titer
Abstract:Objective
To establish and validate the direct immunofluorescence method to detect rabies virus (RV) titer.
Methods
The influences of inoculation method (stepwise and one-step inoculations) on ...Objective
To establish and validate the direct immunofluorescence method to detect rabies virus (RV) titer.
Methods
The influences of inoculation method (stepwise and one-step inoculations) on titer of RV were evaluated by direct immunofluorescence assay. The optimized method was verified for inter-precision and specificity, and the results were compared with those of intracerebral titration in mice.
Results
The cells were inoculated by one-step inoculation.The variation coefficient of 14 test results by two personnel was only 0.04%. Specific fluorescence was observed only in RV but not in non-RV control viruses by the method. The determination results of virus titers by direct immunofluorescence method showed positive correlation with that by intracerebral titration in mice. There is a good linear relationship between the results of the two methods (r=0.918), with linear regression equation: y=0.907x-1.566 (t=12.80, P<0.05).
Conclusion
The optimized direct immunofluorescence method is precise, specific, easy to handle and time-saving, thus can be used for the quantitative determination of RV titer.
Key words:
Rabies virus; Direct immunofluorescence assy; Virus titerRead More
Publication Year: 2017
Publication Date: 2017-10-10
Language: en
Type: article
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