Title: Study of hepatitis C virus core antigen detection in voluntary blood donation
Abstract: Objective
To detect anti-hepatitis C virus enzyme-linked immunoassay (anti-HCV ELISA) positive blood specimens by HCV core antigen ELISA(HCV-cAgELISA), and to investigate the value of detecting of HCV-cAg for screening blood donor.
Methods
All anti-HCV ELISA positive specimens were identified by using HCV-cAgELISA and HCV RT-PCR methods.
Results
In 225 anti-HCV positive specimens, the positive rates of HCV-cAgELISA and HCV RT-PCR were 39.1% and 38.7%, respectively. the positive rates were no significant difference. Among anti-HCV screening unqualified samples, the false positive rates of anti-HCV ELISA in both positive, single positive, and grey area were 31.4%, 90.4% and 83.0%, respectively. The total false positive rate was 61.3%. The false positive rate of HCV-cAgELISA in both positive, single positive, and grey area were 1.4%, 0 and 0, respectively. The total false positive rate was 1.1%. The false positive rate of HCV-cAgELISA was significantly lower anti-HCV ELISA(P<0.01).
Conclusion
Sensitivity of HCVcAg ELISA is silllilar to HCV RT-PCR(P>0.05). The total false positive rate of HCVcAg ELISA was significantly lower anti-HCV ELISA(P<0.01). HCVcAg ELISA is a simple, fast, and reliable memod to screen donor with HCV infection in blood transfusion medicine.
Key words:
Hepatitis C; Blood donor; Hepacivirus
Publication Year: 2015
Publication Date: 2015-02-28
Language: en
Type: article
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