Studies on Herpesvirus of Turkeys : I. Isolation of Herpesvirus from Turkeys and Its Virological Characteristics

Name: Work Video:
Duration: 3 min 30 s
Koichi Ono; Toshiyuki DOI; Toyokazu Ishikawa; Nobuzo Iwa; Matao Naito; Shiro Kato; K Koyama; T Konobe; K Takaku
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'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W3207175092', 'https://openalex.org/W2418109589', 'https://openalex.org/W2414355264', 'https://openalex.org/W2408136030', 'https://openalex.org/W2342741107', 'https://openalex.org/W2327725382', 'https://openalex.org/W2247178823', 'https://openalex.org/W2086347132', 'https://openalex.org/W2025494186', 'https://openalex.org/W2000168546'], 'abstract_inverted_index': {'1970年,': [0], '健康なシチメンチョウ105羽中6羽の血液から,': [1], 'アヒル胎児線維芽細胞を用いて,': [2], 'ヘルペス型ウイルスを分離した.': [3], 'これらのウイルスでは,': [4], 'マレック病ウイルスと異なり,': [5, 54], '細胞遊離ウイルスを容易に得ることができた.': [6], '特にO1株において,': [7], 'その傾向は明瞭であった.': [8], '感染培養細胞には,': [9], 'プラークが認められ,': [10], 'プラークは,': [11], '多数の多核巨細胞より成っていた.': [12], '感染細胞のヘマトキシリン・エオシン染色において,': [13], 'ヘルペス型の核内封入体の形成が認められた.': [14], 'この封入体は,': [15], 'Feulgen': [16], '反応およびアクリジン・オレンジ染色により,': [17], 'DNA': [18, 24], '陽性を示し,': [19], '3H-thymidine': [20], 'のオートラジオグラフィーにおいて,': [21], 'その中にヘルペス型配列の銀粒子が認められた.': [22], 'また': [23], '合成阻害剤に対し感受性を示した.': [25], 'さらに感染細胞の超薄切片を電子顕微鏡で観察すると,': [26], 'ヘルペス型ウイルスの構造をもつ粒子が,': [27], '核内に多数認められた.': [28], 'これら二重膜粒子の直径は約140mμ,': [29], 'カプシドの直径は,': [30], '約100mμであった.': [31], 'しかし,': [32], 'マレック病ウイルス': [33], 'Biken': [34], 'C2': [35], '株感染細胞の場合と異なり,': [36], 'カプシドの内部が十字紋様を呈するものが多数認められた.': [37], 'また,': [38], '感染材料を燐タングステン酸によりネガティブ染色して,': [39], '電子顕微鏡で観察すると,': [40], '中空のカプソメア(推定162個)より成るヘルペス型カプシド構造が認められた.': [41], 'これらのウイルスは,': [42], '分離当初よリアヒルおよびウズラ胎児線維芽細胞上におけるプラーク形成が明瞭であり,': [43], '培養には多核巨細胞も出現した.': [44], 'アヒル,': [45], 'ウズラおよびニワトリ胎児線維芽細胞は,': [46], 'このウイルスに対して,': [47], 'ほぼ同一の感受性を示した.': [48], '螢光抗体法により,': [49], 'マレック病ウイルスと交差反応を呈したが,': [50], '同一反応ではなかった.': [51], '以上の研究により,': [52], '本ウイルスは,': [53], 'Witter': [55], 'らの報告したシチメンチョウ・ヘルペスウイルスと性格がほぼ一致するものと考えられる.': [56], '本研究の要旨は,': [57], '第71回(1971年4月)および第72回(1971年10月)日本獣医学会において報告した.': [58], '終わりに臨み,': [59], '電子顕微鏡による研究について,': [60], '御指導御協力下さった当研究室新居志郎助教授,': [61], '大阪医科大学内藤継也氏,': [62], 'ならびに本研究に際し,': [63], '種々御協力を賜わった兵庫県経済連藤田重夫部長,': [64], '小馬栄猛氏,': [65], '岸田満須夫氏,': [66], 'および柏原農協永井明男氏に深謝する.': [67]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W3026233374', 'counts_by_year': [], 'updated_date': '2024-12-16T17:48:15.907874', 'created_date': '2020-05-29'}
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