Title: Derivation and Differentiation of Human Embryonic Germ Cells
Abstract: Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. Like mouse embryonic stem (ES) and EG cells, human EG cells require leukemia inhibitory factor (LIF) for proliferation as undifferentiated stem cells. Unlike mouse EG cells, however, human EG cells retain dependence on exogenous cytokines and factors supplied by the feeder layer, and spontaneously differentiate into embryoid bodies (EBs) more frequently. Although EBs are not pluripotent, they express phenotypic markers found on progenitor, precursor, and mature cells. Cells retaining a capacity for cell proliferation and express makers of multiple lineages can be isolated from EBs, and can be used in a variety of in vitro and in vivo differentiation paradigms. Current challenges are to match individual EB-derived (EBD) cultures to desired endpoints, and to enrich or purify specific populations of cells within EBD cultures.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 1
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