Title: Effect of Proton Pump Inhibitors, Vitamin E and their co- administration on heart function and Oxidative Changes in Isoprenaline Induced Myocardial Infarction in Adult Male Albino Rats
Abstract: Background: Myocardial infarction (MI) continues to be associated with high morbidity and mortality worldwide.Proton Pump inhibitors (PPIs) exhibit cardioprotective effects by several mechanisms.Vitamin E possesses antioxidant properties.Aim: The present study has been designed to evaluate the combined cardioprotective effect of PPIs and vitamin E on biochemical and histopathological alteration in isoprenaline (ISO) induced myocardial infarction in rats.Methods: 30 adult Wistar albino male rats divided into 5 groups as follow: Group I: Control fed a standard diet.Group II: rats were undergoing MI by ISO.Group III: Rats were pretreated pantoprazole (1.3 mg/kg, i.o) daily for 3 weeks.Group IV: Rats were pretreated with vitamin E (100 IU/kg i.o) daily for 3 weeks.Group V: Rats were given both pantoprazole (1.3 mg/kg, i.o) and vitamin E (100 IU/kg bw, i.p) daily.On 22 th and 23 th day, rats in groups (II-V) were injection i.p with ISO (100mg/kg bw for successive two days).Results: ISO induced infarction in rats resulted in a significant elevation in ST segment height, Twave voltage, Heart rate (HR), serum lactate dehydrogenase (LDH), malondialdehyde (MDA) and nitric oxide metabolites (NO), whereas it caused a significant (P < 0.05) decrease in systolic blood pressure(SBP) and serum superoxide dismutase (SOD) when compared with the control group.Moreover, interventions with PPIs supplementation and/or vitamin E significantly reversed the aforementioned parameters with better impact of their combination.Conclusion: PPIs combined with vitamin E exert potential protective effect against MI through lowering calcium overload and enhancement of antioxidant activity.