Abstract: Abstract The fatty acid composition of the oil extracted from tomato seeds was determined by GLC. A urea fractionation procedure and preparative GLC were used to detect esters present in small or trace amounts. Linoleic, oleic, and palmitic acids were predominant. Odd- and even-chain length saturated acids from C12-C28 were present. Hexadecenoic, heptadecenoic, and eicosenoic acids were also detected. Minor chromatographic peaks preceding some of the saturated esters were attributed to branched acids.