Title: Molecular and functional characterization of <i>Raptor</i> in mTOR pathway from <i>Litopenaeus vannamei</i>
Abstract: Aquaculture ResearchVolume 51, Issue 6 p. 2179-2189 ORIGINAL ARTICLE Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei Han Fang, Han Fang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China University of Chinese Academy of Sciences, Beijing, ChinaSearch for more papers by this authorXinwei Liu, Xinwei Liu University of Amsterdam, Amsterdam, NetherlandsSearch for more papers by this authorXuqing Shao, Xuqing Shao Shandong Cigna Detection Technology Co., Ltd, Qingdao, ChinaSearch for more papers by this authorFang Xin, Fang Xin CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China University of Chinese Academy of Sciences, Beijing, ChinaSearch for more papers by this authorBaojie Wang, Baojie Wang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMengqiang Wang, Mengqiang Wang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorKeyong Jiang, Keyong Jiang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMei Liu, Corresponding Author Mei Liu [email protected] CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mei Liu and Lei Wang, CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Emails: [email protected]; [email protected];Search for more papers by this authorLei Wang, Corresponding Author Lei Wang [email protected] orcid.org/0000-0003-2553-0318 CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mei Liu and Lei Wang, CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Emails: [email protected]; [email protected];Search for more papers by this author Han Fang, Han Fang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China University of Chinese Academy of Sciences, Beijing, ChinaSearch for more papers by this authorXinwei Liu, Xinwei Liu University of Amsterdam, Amsterdam, NetherlandsSearch for more papers by this authorXuqing Shao, Xuqing Shao Shandong Cigna Detection Technology Co., Ltd, Qingdao, ChinaSearch for more papers by this authorFang Xin, Fang Xin CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China University of Chinese Academy of Sciences, Beijing, ChinaSearch for more papers by this authorBaojie Wang, Baojie Wang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMengqiang Wang, Mengqiang Wang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorKeyong Jiang, Keyong Jiang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSearch for more papers by this authorMei Liu, Corresponding Author Mei Liu [email protected] CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mei Liu and Lei Wang, CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Emails: [email protected]; [email protected];Search for more papers by this authorLei Wang, Corresponding Author Lei Wang [email protected] orcid.org/0000-0003-2553-0318 CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Correspondence Mei Liu and Lei Wang, CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Emails: [email protected]; [email protected];Search for more papers by this author First published: 23 January 2020 https://doi.org/10.1111/are.14522Citations: 2 Fang and Liu authors have contributed equally to this work and are considered as co-first authors. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Raptor, a member of the target of rapamycin complex 1 (TORC1), participates in the formation of complex proteins related to the mechanistic target of rapamycin (mTOR) signalling. In this study, a 5,020 bp cDNA of Raptor with an open reading frame (ORF) of 3,804 bp encoding for 1,267 amino acids was cloned from Litopenaeus vannamei. The protein contains three conserved domains: Raptor N, HEAT and WD40 domains. The expression of Raptor gene was detected by qRT-PCR in different tissues of L. vannamei, including hepatopancreas, intestinal, stomach, eyestalk, gill and muscle. The mRNA expression profiles of Raptor in muscle were also analysed under suppression or stimulation of mTOR signalling pathway. The level of Raptor mRNA significantly increased either at 0.5–6 hr after an injection of rapamycin (RAPA) or after 3 days starvation. Leucine or arginine alleviated the up-regulation of Raptor gene expression caused by RAPA or starvation. The Raptor gene was successfully suppressed using RNA interference (RNAi) technology, and the gene expression and the protein phosphorylation level of 4EBP1 and S6K were significantly decreased. The results of the study suggested that the expression of Raptor was sensitive to the immunology status of L. vannamei and participated in nutritional metabolism. CONFLICT OF INTEREST The authors declare that there is no conflict of interest. Open Research DATA AVAILABILITY STATEMENT I confirm that my article contains a Data Availability Statement even if no data are available unless my article type does not require one. I confirm that I have included a citation for available data in my reference section, unless my article type is exempt. The data that support the findings of this study are available from the corresponding author upon reasonable request. Citing Literature Volume51, Issue6June 2020Pages 2179-2189 RelatedInformation