Title: Ultrasound-Assisted Extraction and Optimization Process Parameters of Antioxidant and Phenolic Compounds from Myristica fragrans
Abstract: Background: Ultrasonic extraction is one method for optimization of effective compounds from plants. Objectives: The aim of this study was optimization of the extraction conditions of <i>Myristica fragrans</i> seeds using ethanol as a food grade solvent and by ultrasonic-assisted extraction using response surface method (RSM). Methods: Ultrasonic-assisted extraction was used for extraction of <i>M. fragrans</i> seeds. Response surface methodology (RSM) was used to optimize three parameters: time and of temperature extraction (min, °C), and solvent to material ratio. Antioxidant activity and phenolic contents were determined. Results: The results showed that optimal ultrasonic-assisted extraction (UAE) conditions were 29.57 minutes extraction time, 374.61 mL/g ratio of solvent to material and 41.89ºC extraction temperatures. The yield of extraction, amount of total phenolic content, IC<sub>50</sub> (in DPPH method) and FRAP in <i>M. fragrans</i> seeds in optimum conditions were 18.14%, 63.41 mg gallic acid/g of extract, 43.66 mg/mL and 99.38 mmol/g of extract, respectively. Conclusions: The results indicated of the ultrasonic-assisted extraction having power for optimization of the extraction condition and it is appropriate for extraction of antioxidant and phenolic compounds.