Title: A NEW SCAR MARKER OF HIGH SUGAR GENE IN SWEET SORGHUM [SORGHUM BICOLOR (L.) MOENCH]
Abstract: This experiment used molecular marker technology to analyse high sugar genes, clone the target bands and the Random amplified polymorphic DNA (RAPD) molecular markers were converted into Sequence characterized amplified regions (SCAR) molecular markers, and additionally 120 RAPD primers were used to analyse female 3228B (sweet sorghum), paternal 3201B (ordinary sorghum) and their hybrids F5.The results showed that 95 of 120 pairs amplified products, 25 of them did not amplify products, so the amplification rate reached 79%.The recombination rate of S512 and high sugar genes was 7% in F5 populations.S512 amplified polymorphic bands were recovered, cloned and sequenced, its band length was 441bp.According to the sequence, we transferred the RAPD to SCAR molecular markers and designed SCAR primer.We got the same amplification results with the RAPD in specific amplification progress of sweet sorghum F5 populations, thus the RAPD transfered to SCAR successfully, we called this SCARS512-441.