Title: Cloning and assay of regulating sequence of the rat 20alphaHSD gene
Abstract: Digesting the lambda-phage gene which may carry the promoter region of 20 alphaHSD, we obtained a 4.0 kb fragment. The fragment was subcloned into p~Bluescript vector, resulting in a fusion constructs. Digesting the constructs and making southern hybridization with the cDNA of rat 20 alphaHSD labeled with DIG, we revealed the 4.0 kb fragment having been inserted into 2.9 kb vector using the BamH site.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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