Title: Adventitious Bud Induction and Plantlet Regeneration in vitro from Mature Zygotic Embryos of Ponderosa Pine
Abstract: Adventitious buds were induced from the excised mature zygotic embryos of Ponderosa Pine (Pinus ponderosa). The highest induction rate (65.8%) of adventitious buds was obtained with 10-fold multiplication at the highest and 7-fold multiplication at an average when mature zygotic embryos were cultured on GD + 9.85~19.70 μmol·L-1 6-BA+ 0.0~14.42 μmol·L-1 NAA. The adventitious buds were initiated or formed directly from the cotyledons. Additional NAA inhibited the formation of adventitious buds. Buds proliferation and elongation were achieved on 1/2GD and 1/2SH without growth regulators. A proper amount of activated charcoal promoted adventitious buds elongation and root growth. The adventitious shoots of Ponderosa Pine were used to induce adventitious roots on 1/2GD and 1/2SH media supplemented with different concentrations of NAA and GA3. The results showed that NAA played a determinative role in initiating the adventitious roots. Sixteen point seven percent (16.7%) of adventitious shoots was rooted on 1/2GD with 28.84 μmol·L-1 NAA+4.17 μmol·L-1 GA3. In the present paper, the experimental results showed that roots were successfully induced from cultured mature zygotic embryos of Ponderosa Pine.
Publication Year: 2008
Publication Date: 2008-05-25
Language: en
Type: article
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