Title: Determination of grapefruit (<i>Citrus paradisi</i>) peel extract bio‐active substances and its application in Caspian white fish (<i>Rutilus frisii kutum</i>) diet: Growth, haemato‐biochemical parameters and intestinal morphology
Abstract: Aquaculture ResearchVolume 50, Issue 9 p. 2496-2504 ORIGINAL ARTICLE Determination of grapefruit (Citrus paradisi) peel extract bio-active substances and its application in Caspian white fish (Rutilus frisii kutum) diet: Growth, haemato-biochemical parameters and intestinal morphology Zahra Samavat, Zahra Samavat Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, IranSearch for more papers by this authorMehdi Shamsaie Mehrgan, Corresponding Author Mehdi Shamsaie Mehrgan [email protected] orcid.org/0000-0002-2445-853X Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Correspondence Mehdi Shamsaie Mehrgan, Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Email: [email protected] for more papers by this authorShahla Jamili, Shahla Jamili Iranian Fisheries Research Organization, Agricultural Research Education and Extension Organization (AREEO), Tehran, IranSearch for more papers by this authorMehdi Soltani, Mehdi Soltani orcid.org/0000-0002-5367-7133 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Freshwater and Fish Health Group, Centre for Sustainable Aquatic Ecosystems, Harry Butler Institute, Murdoch University, Murdoch, Western Australia, AustraliaSearch for more papers by this authorSeyed Pezhman Hosseini Shekarabi, Seyed Pezhman Hosseini Shekarabi orcid.org/0000-0002-6407-4004 Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, IranSearch for more papers by this author Zahra Samavat, Zahra Samavat Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, IranSearch for more papers by this authorMehdi Shamsaie Mehrgan, Corresponding Author Mehdi Shamsaie Mehrgan [email protected] orcid.org/0000-0002-2445-853X Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Correspondence Mehdi Shamsaie Mehrgan, Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Email: [email protected] for more papers by this authorShahla Jamili, Shahla Jamili Iranian Fisheries Research Organization, Agricultural Research Education and Extension Organization (AREEO), Tehran, IranSearch for more papers by this authorMehdi Soltani, Mehdi Soltani orcid.org/0000-0002-5367-7133 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Freshwater and Fish Health Group, Centre for Sustainable Aquatic Ecosystems, Harry Butler Institute, Murdoch University, Murdoch, Western Australia, AustraliaSearch for more papers by this authorSeyed Pezhman Hosseini Shekarabi, Seyed Pezhman Hosseini Shekarabi orcid.org/0000-0002-6407-4004 Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, IranSearch for more papers by this author First published: 05 June 2019 https://doi.org/10.1111/are.14204Citations: 13 Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In this study, total phenolic and flavonoid contents of grapefruit peel extract (GPE) were equal to 117.3 ± 0.3 µg of gallic acid/mg and 39.30 ± 0.1 µg of quercetin/mg respectively. Caspian white fish (n = 180, 4 ± 0.9 g body weight) was fed with supplemented diets, including 0, 6.25, 12.5 and 25 mg of GPE/kg for 60 days at 25 ± 1°C. The growth performance was markedly improved in fish fed with 25 mg/kg of GPE compared to others (p < 0.05). Moreover, fish fed with 25 mg/kg of GPE showed a significant increase in red blood cell (2.65·106 cell/mm3), white blood cell (17.75·103 cell/mm3), packed cell volume (48%) and haemoglobin concentrations (8.75 g/dl) compared to the control (p < 0.05). However, the highest alanine aminotransferase (140 U/L), alanine transaminase (14.5 U/L), and alkaline phosphatase (18.5 U/L) were observed in control group. Morphological analysis of intestine revealed the highest amount of villus width (8.4 µM), height (32.86 µM) and surface area (342.7 µM2) in fish fed with 25 mg/kg of GPE (p < 0.05). In conclusion, supplementing feed with GPE at 25 g/kg can improve growth performance and haemato-biochemical parameters of Caspian white fish fry. CONFLICTS OF INTEREST The authors declare that there are no conflicts of interest. Citing Literature Volume50, Issue9September 2019Pages 2496-2504 RelatedInformation