Title: A novel H7N3 reassortant originating from the zoonotic H7N9 highly pathogenic avian influenza viruses that has adapted to ducks
Abstract: Transboundary and Emerging DiseasesVolume 66, Issue 6 p. 2342-2352 ORIGINAL ARTICLE A novel H7N3 reassortant originating from the zoonotic H7N9 highly pathogenic avian influenza viruses that has adapted to ducks Momoko Nakayama, Momoko Nakayama orcid.org/0000-0002-9284-8425 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorYuko Uchida, Yuko Uchida orcid.org/0000-0002-5833-5917 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorAkihiro Shibata, Akihiro Shibata Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorYoshifumi Kobayashi, Yoshifumi Kobayashi Pathological and Physiochemical Examination Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, JapanSearch for more papers by this authorJunki Mine, Junki Mine Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorNobuhiro Takemae, Nobuhiro Takemae orcid.org/0000-0001-5152-8938 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorRyota Tsunekuni, Ryota Tsunekuni orcid.org/0000-0002-6204-1233 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorTaichiro Tanikawa, Taichiro Tanikawa Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorRieko Harada, Rieko Harada Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorHiroyuki Osaka, Hiroyuki Osaka Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorTakehiko Saito, Corresponding Author Takehiko Saito [email protected] Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan Correspondence Takehiko Saito, Tsukuba, Ibaraki, 305-0056 Japan. Email: [email protected] for more papers by this author Momoko Nakayama, Momoko Nakayama orcid.org/0000-0002-9284-8425 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorYuko Uchida, Yuko Uchida orcid.org/0000-0002-5833-5917 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorAkihiro Shibata, Akihiro Shibata Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorYoshifumi Kobayashi, Yoshifumi Kobayashi Pathological and Physiochemical Examination Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, JapanSearch for more papers by this authorJunki Mine, Junki Mine Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorNobuhiro Takemae, Nobuhiro Takemae orcid.org/0000-0001-5152-8938 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorRyota Tsunekuni, Ryota Tsunekuni orcid.org/0000-0002-6204-1233 Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorTaichiro Tanikawa, Taichiro Tanikawa Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, JapanSearch for more papers by this authorRieko Harada, Rieko Harada Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorHiroyuki Osaka, Hiroyuki Osaka Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Tokoname, JapanSearch for more papers by this authorTakehiko Saito, Corresponding Author Takehiko Saito [email protected] Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan Correspondence Takehiko Saito, Tsukuba, Ibaraki, 305-0056 Japan. Email: [email protected] for more papers by this author First published: 11 July 2019 https://doi.org/10.1111/tbed.13291Citations: 8Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The first human case of zoonotic H7N9 avian influenza virus (AIV) infection was reported in March 2013 in China. This virus continues to circulate in poultry in China while mutating to highly pathogenic AIVs (HPAIVs). Through monitoring at airports in Japan, a novel H7N3 reassortant of the zoonotic H7N9 HPAIVs, A/duck/Japan/AQ-HE30-1/2018 (HE30-1), was detected in a poultry meat product illegally brought by a passenger from China into Japan. We analysed the genetic, pathogenic and antigenic characteristics of HE30-1 by comparing it with previous zoonotic H7N9 AIVs and their reassortants. Phylogenetic analysis of the entire HE30-1 genomic sequence revealed that it comprised at least three different sources; the HA (H7), PB1, PA, NP, M and NS segments of HE30-1 were directly derived from H7N9 AIVs, whereas the NA (N3) and PB2 segments of HE30-1 were unrelated to zoonotic H7N9. Experimental infection revealed that HE30-1 was lethal in chickens but not in domestic or mallard ducks. HE30-1 was shed from and replicated in domestic and mallard ducks and chickens, whereas previous zoonotic H7N9 AIVs have not adapted well to ducks. This finding suggests the possibility that HE30-1 may disseminate to remote area by wild bird migration once it establishes in wild bird population. A haemagglutination-inhibition assay indicated that antigenic drift has occurred among the reassortants of zoonotic H7N9 AIVs; HE30-1 showed similar antigenicity to some of those H7N9 AIVs, suggesting it might be prevented by the H5/H7 inactivated vaccine that was introduced in China in 2017. Our study reports the emergence of a new reassortant of zoonotic H7N9 AIVs with novel viral characteristics and warns of the challenge we still face to control the zoonotic H7N9 AIVs and their reassortants. CONFLICT OF INTEREST The authors declare that they have no competing interests. Citing Literature Supporting Information Filename Description tbed13291-sup-0001-Figures.pdfPDF document, 219 KB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume66, Issue6November 2019Pages 2342-2352 RelatedInformation