Title: Silencing miR‐21 induces polarization of astrocytes to the A2 phenotype and improves the formation of synapses by targeting glypican 6 <i>via</i> the signal transducer and activator of transcription‐3 pathway after acute ischemic spinal cord injury
Abstract: The FASEB JournalVolume 33, Issue 10 p. 10859-10871 ResearchFree to Read Silencing miR-21 induces polarization of astrocytes to the A2 phenotype and improves the formation of synapses by targeting glypican 6 via the signal transducer and activator of transcription-3 pathway after acute ischemic spinal cord injury Yanlin Su, Yanlin Su Jinan Central Hospital, Shandong University, Jinan, China Shandong First Medical University, Taian, ChinaSearch for more papers by this authorZhe Chen, Zhe Chen School of Physical Education and Sports Science, South China Normal University, Guangzhou, ChinaSearch for more papers by this authorHongxia Du, Hongxia Du Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorRonghan Liu, Ronghan Liu Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorWenzhao Wang, Wenzhao Wang Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorHongfei Li, Hongfei Li Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorBin Ning, Corresponding Author Bin Ning [email protected] Jinan Central Hospital, Shandong University, Jinan, China Correspondence: Jinan Central Hospital, Shandong University, No. 105, Jiefang Rd., Jinan 250013, China. E-mail: [email protected]Search for more papers by this author Yanlin Su, Yanlin Su Jinan Central Hospital, Shandong University, Jinan, China Shandong First Medical University, Taian, ChinaSearch for more papers by this authorZhe Chen, Zhe Chen School of Physical Education and Sports Science, South China Normal University, Guangzhou, ChinaSearch for more papers by this authorHongxia Du, Hongxia Du Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorRonghan Liu, Ronghan Liu Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorWenzhao Wang, Wenzhao Wang Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorHongfei Li, Hongfei Li Jinan Central Hospital, Shandong University, Jinan, ChinaSearch for more papers by this authorBin Ning, Corresponding Author Bin Ning [email protected] Jinan Central Hospital, Shandong University, Jinan, China Correspondence: Jinan Central Hospital, Shandong University, No. 105, Jiefang Rd., Jinan 250013, China. E-mail: [email protected]Search for more papers by this author First published: 02 July 2019 https://doi.org/10.1096/fj.201900743RCitations: 3Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT Ischemic spinal cord injury (ISCI) results in the motor sensory dysfunction of the limbs below the injury site. In response to the injury, astrocytes develop into neuroprotective astrocytes [(neurotrophic reactive astrocytes (A2s)] to mitigate the damage. MicroRNA (miR)-21 can promote the development of neuroinflammation in previous studies. Our aim was to investigate the effect of miR-21 on its polarization. We used the abdominal aortic occlusion model in vivo. Immunohistochemistry was used to detect the distribution of A2s in the spinal cord. We used an oxygen glucose deprivation method to model astrocytes ischemia in vitro and tested proliferation, migration, and excitability of A2s using an 5-ethynyl -2′-deoxyuridine kit, wound scratch assay, and calcium-ion probe. After adjustment, we detected the model and target genes of A2s using PCR Western blot, immunofluorescence, and chromatin immunoprecipitation. We demonstrated in vivo that naive astrocytes were transformed into A2s by ischemia. And in vitro miR-21, which can regulate the signal transducer and activator of transcription-3 pathway, can transform neurotoxic reactive astrocyte into A2. Moreover, we also verified the mechanism of A2s promoting synaptic formation and nerve growth. miR-21 is a switch to regulate the polarization of reactive astrocyte, and it promoted synapsis formation and nerites growth after acute ISCI.—Su, Y., Chen, Z., Du, H., Liu, R., Wang, W., Li, H., Ning, B. Silencing miR-21 induces polarization of astrocytes to the A2 phenotype and improves the formation of synapses by targeting glypican 6 via the signal transducer and activator of transcription-3 pathway after acute ischemic spinal cord injury. FASEB J. 33, 10859–10871 (2019). www.fasebj.org Citing Literature Volume33, Issue10October 2019Pages 10859-10871 RelatedInformation
Publication Year: 2019
Publication Date: 2019-07-02
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 64
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