Title: Properties of Transmembrane Channels Induced by Cytolytic Lymphocytes
Abstract:Cloned cell lines with high cytotoxic activity have become available. It has become possible to grow large numbers of homogeneous populations of cells for biochemical and biophysical studies. This cha...Cloned cell lines with high cytotoxic activity have become available. It has become possible to grow large numbers of homogeneous populations of cells for biochemical and biophysical studies. This chapter reviews the cytolytic properties associated with the cytoplasmic granules isolated from these cells and with the protein responsible for the tubular lesions produced on target cell membranes. Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells lyse tumor cells by a contact- dependent mechanism. The chapter aims to isolate an active principle from CTL and NK cells that could explain the mechanism of the cytolysis observed with intact cells. A planar bilayer, prepared by the technique of M. Montai and P. Mueller, is exposed to granular material derived from mouse CTL. The putative pore-former from the cytolytic T cell granules has been named cytolysin and perforin. The cytolytic protein localized in the granules is released into the intercellular space following the effector-target cell contact.Read More
Publication Year: 2018
Publication Date: 2018-01-18
Language: en
Type: book-chapter
Indexed In: ['crossref']
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