Title: Analysis of tartary buckwheat ( <i>Fagopyrum tataricum</i> ) seed proteome using offline two‐dimensional liquid chromatography and tandem mass spectrometry
Abstract: Journal of Food BiochemistryVolume 43, Issue 7 e12863 FULL ARTICLE Analysis of tartary buckwheat (Fagopyrum tataricum) seed proteome using offline two-dimensional liquid chromatography and tandem mass spectrometry Jinqiu Wang, Jinqiu Wang orcid.org/0000-0003-1736-3652 Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorJing Xiao, Jing Xiao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorXin Liu, Xin Liu Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorFang Geng, Corresponding Author Fang Geng [email protected] orcid.org/0000-0002-8275-6770 Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. China Correspondence Fang Geng, Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, P.R. China. Email: [email protected] for more papers by this authorQun Huang, Qun Huang Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, P.R. ChinaSearch for more papers by this authorJianglin Zhao, Jianglin Zhao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorDabing Xiang, Dabing Xiang Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorGang Zhao, Gang Zhao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this author Jinqiu Wang, Jinqiu Wang orcid.org/0000-0003-1736-3652 Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorJing Xiao, Jing Xiao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorXin Liu, Xin Liu Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorFang Geng, Corresponding Author Fang Geng [email protected] orcid.org/0000-0002-8275-6770 Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. China Correspondence Fang Geng, Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu 610106, P.R. China. Email: [email protected] for more papers by this authorQun Huang, Qun Huang Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, P.R. ChinaSearch for more papers by this authorJianglin Zhao, Jianglin Zhao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorDabing Xiang, Dabing Xiang Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this authorGang Zhao, Gang Zhao Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, P.R. ChinaSearch for more papers by this author First published: 26 April 2019 https://doi.org/10.1111/jfbc.12863Citations: 21Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The whole seed of tartary buckwheat (Fagopyrum tataricum) is considered as a healthy and functional food, which is rich in kinds of flavonoids and with potential antioxidant effect. An in-depth analysis of tartary buckwheat seed (TBS) proteome was performed using a shotgun proteomics strategy. Total protein of TBS was extracted and digested, then the peptides were separated by offline two-dimensional liquid chromatography and identified by tandem mass spectrometry. Total of 3,363 high-confidence proteins were identified from 13,730 matched peptides, in which, 2,499 proteins were annotated by the Gene Ontology (GO) analysis with 1,720 involved in “biological process,” 2,241 in “molecular function,” and 693 in “cellular components.” Based on the GO functional enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment results, buckwheat seed proteins were mostly enriched in metabolism of nucleic acid, respiration and energy metabolism, as well as synthesis and metabolism of protein. Practical applications This study characterized the tartary buckwheat seed proteome on a scale of 3,000+ proteins and provide important information and clues for future research, especially in the mechanism of seed germination, nutrient composition changes, and metabolite production seed germination and material metabolism. CONFLICT OF INTEREST The authors declared that that have no conflict of interest. Citing Literature Supporting Information Filename Description jfbc12863-sup-0001-TableS1.xlsxapplication/xlsx, 331.8 KB jfbc12863-sup-0002-TableS2.xlsxapplication/xlsx, 652.9 KB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume43, Issue7July 2019e12863 RelatedInformation