Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2922252081', 'doi': 'https://doi.org/10.1007/s10295-019-02159-5', 'title': 'Production of medium chain length polyhydroxyalkanoate from acetate by engineered <i>Pseudomonas putida</i> KT2440', 'display_name': 'Production of medium chain length polyhydroxyalkanoate from acetate by engineered <i>Pseudomonas putida</i> KT2440', 'publication_year': 2019, 'publication_date': '2019-06-01', 'ids': {'openalex': 'https://openalex.org/W2922252081', 'doi': 'https://doi.org/10.1007/s10295-019-02159-5', 'mag': '2922252081', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/30864026'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/s10295-019-02159-5', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S107262989', 'display_name': 'Journal of Industrial Microbiology & Biotechnology', 'issn_l': '1367-5435', 'issn': ['1367-5435', '1476-5535'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5102374979', 'display_name': 'Songyuan Yang', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I162868743', 'display_name': 'Tianjin University', 'ror': 'https://ror.org/012tb2g32', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I162868743']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Songyuan Yang', 'raw_affiliation_strings': ['0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China'], 'affiliations': [{'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}, {'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5011756240', 'display_name': 'Suhang Li', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I162868743', 'display_name': 'Tianjin University', 'ror': 'https://ror.org/012tb2g32', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I162868743']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Suhang Li', 'raw_affiliation_strings': ['0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China'], 'affiliations': [{'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}, {'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5102872642', 'display_name': 'Xiaoqiang Jia', 'orcid': 'https://orcid.org/0000-0002-6125-6486'}, 'institutions': [{'id': 'https://openalex.org/I162868743', 'display_name': 'Tianjin University', 'ror': 'https://ror.org/012tb2g32', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I162868743']}, {'id': 'https://openalex.org/I4210151209', 'display_name': 'Collaborative Innovation Center of Chemical Science and Engineering Tianjin', 'ror': 'https://ror.org/0225a5s12', 'country_code': 'CN', 'type': 'facility', 'lineage': ['https://openalex.org/I162868743', 'https://openalex.org/I205237279', 'https://openalex.org/I4210151209']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Xiaoqiang Jia', 'raw_affiliation_strings': ['0000 0004 1761 2484 grid.33763.32 Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) 300072 Tianjin People’s Republic of China', '0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China'], 'affiliations': [{'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Department of Biological Engineering, School of Chemical Engineering and Technology Tianjin University 300072 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}, {'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology Tianjin University 300350 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I162868743']}, {'raw_affiliation_string': '0000 0004 1761 2484 grid.33763.32 Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) 300072 Tianjin People’s Republic of China', 'institution_ids': ['https://openalex.org/I4210151209']}]}], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2625, 'currency': 'USD', 'value_usd': 2625, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 2.291, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 49, 'citation_normalized_percentile': {'value': 0.673194, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 97, 'max': 98}, 'biblio': {'volume': '46', 'issue': '6', 'first_page': '793', 'last_page': '800'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10661', 'display_name': 'Biodegradable Polymers as Biomaterials and Packaging', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2502', 'display_name': 'Biomaterials'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10661', 'display_name': 'Biodegradable Polymers as Biomaterials and Packaging', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2502', 'display_name': 'Biomaterials'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10404', 'display_name': 'Enzyme Immobilization Techniques', 'score': 0.9873, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10932', 'display_name': 'Metabolic Engineering and Synthetic Biology', 'score': 0.9553, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/pseudomonas-putida', 'display_name': 'Pseudomonas putida', 'score': 0.99712324}, {'id': 'https://openalex.org/keywords/polyhydroxyalkanoates', 'display_name': 'Polyhydroxyalkanoates', 'score': 0.544959}, {'id': 'https://openalex.org/keywords/polyester-synthases', 'display_name': 'Polyester Synthases', 'score': 0.503492}, {'id': 'https://openalex.org/keywords/metabolic-engineering', 'display_name': 'Metabolic engineering', 'score': 0.44350368}, {'id': 'https://openalex.org/keywords/acetate-kinase', 'display_name': 'Acetate kinase', 'score': 0.4204697}], 'concepts': [{'id': 'https://openalex.org/C2780543182', 'wikidata': 'https://www.wikidata.org/wiki/Q2738168', 'display_name': 'Pseudomonas putida', 'level': 3, 'score': 0.99712324}, {'id': 'https://openalex.org/C73447357', 'wikidata': 'https://www.wikidata.org/wiki/Q425079', 'display_name': 'Polyhydroxyalkanoates', 'level': 3, 'score': 0.8461588}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.5448681}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.5030026}, {'id': 'https://openalex.org/C6350086', 'wikidata': 'https://www.wikidata.org/wiki/Q6822347', 'display_name': 'Metabolic engineering', 'level': 3, 'score': 0.44350368}, {'id': 'https://openalex.org/C2781463711', 'wikidata': 'https://www.wikidata.org/wiki/Q131143', 'display_name': 'Pseudomonas', 'level': 3, 'score': 0.4363267}, {'id': 'https://openalex.org/C2779826215', 'wikidata': 'https://www.wikidata.org/wiki/Q4673250', 'display_name': 'Acetate kinase', 'level': 4, 'score': 0.4204697}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.38875693}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.35459828}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.31179142}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.19823086}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.07208553}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D000085', 'descriptor_name': 'Acetates', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D060847', 'descriptor_name': 'Metabolic Engineering', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D054813', 'descriptor_name': 'Polyhydroxyalkanoates', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D016958', 'descriptor_name': 'Pseudomonas putida', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D000085', 'descriptor_name': 'Acetates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D061249', 'descriptor_name': 'Batch Cell Culture Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D060847', 'descriptor_name': 'Metabolic Engineering', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D054813', 'descriptor_name': 'Polyhydroxyalkanoates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016958', 'descriptor_name': 'Pseudomonas putida', 'qualifier_ui': 'Q000254', 'qualifier_name': 'growth & development', 'is_major_topic': False}, {'descriptor_ui': 'D016958', 'descriptor_name': 'Pseudomonas putida', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/s10295-019-02159-5', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S107262989', 'display_name': 'Journal of Industrial Microbiology & Biotechnology', 'issn_l': '1367-5435', 'issn': ['1367-5435', '1476-5535'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/30864026', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [{'funder': 'https://openalex.org/F4320321001', 'funder_display_name': 'National Natural Science Foundation of China', 'award_id': '21576197'}, {'funder': 'https://openalex.org/F4320335777', 'funder_display_name': 'National Key Research and Development Program of China', 'award_id': '2014CB745100'}], 'datasets': [], 'versions': [], 'referenced_works_count': 49, 'referenced_works': ['https://openalex.org/W1497101056', 'https://openalex.org/W1533868191', 'https://openalex.org/W1935931219', 'https://openalex.org/W1964184380', 'https://openalex.org/W1970253937', 'https://openalex.org/W1987499909', 'https://openalex.org/W1993824656', 'https://openalex.org/W1996186327', 'https://openalex.org/W1998194018', 'https://openalex.org/W2009220402', 'https://openalex.org/W2023188336', 'https://openalex.org/W2024835614', 'https://openalex.org/W2036917159', 'https://openalex.org/W2048439294', 'https://openalex.org/W2054920102', 'https://openalex.org/W2080420830', 'https://openalex.org/W2080803897', 'https://openalex.org/W2088547619', 'https://openalex.org/W2089048992', 'https://openalex.org/W2089848749', 'https://openalex.org/W2092125847', 'https://openalex.org/W2099321243', 'https://openalex.org/W2099500242', 'https://openalex.org/W2101737102', 'https://openalex.org/W2107277218', 'https://openalex.org/W2113353236', 'https://openalex.org/W2117282430', 'https://openalex.org/W2118753241', 'https://openalex.org/W2134153749', 'https://openalex.org/W2144634347', 'https://openalex.org/W2152875113', 'https://openalex.org/W2157547677', 'https://openalex.org/W2167114748', 'https://openalex.org/W2171219265', 'https://openalex.org/W219670351', 'https://openalex.org/W2278253772', 'https://openalex.org/W2294471624', 'https://openalex.org/W2338202395', 'https://openalex.org/W2339423968', 'https://openalex.org/W2489871023', 'https://openalex.org/W2490034073', 'https://openalex.org/W2523354609', 'https://openalex.org/W2612500845', 'https://openalex.org/W2766454613', 'https://openalex.org/W2804007336', 'https://openalex.org/W2810508147', 'https://openalex.org/W2911297331', 'https://openalex.org/W4211245217', 'https://openalex.org/W4230789027'], 'related_works': ['https://openalex.org/W3164354965', 'https://openalex.org/W3120814900', 'https://openalex.org/W3012829536', 'https://openalex.org/W2941710292', 'https://openalex.org/W2462005841', 'https://openalex.org/W2171219265', 'https://openalex.org/W2112779437', 'https://openalex.org/W2104654451', 'https://openalex.org/W2080169869', 'https://openalex.org/W2021225207'], 'abstract_inverted_index': {'Pseudomonas': [0, 40], 'putida': [1, 34, 43, 46, 69, 101, 127], 'was': [2, 36, 71], 'metabolically': [3], 'engineered': [4, 72, 144], 'to': [5, 18, 27, 30, 59], 'produce': [6], 'medium': [7], 'chain': [8], 'length': [9], 'polyhydroxyalkanoate': [10], '(mcl-PHA)': [11], 'from': [12, 38], 'acetate,': [13], 'a': [14, 104, 135], 'promising': [15], 'carbon': [16], 'source': [17], 'achieve': [19], 'cost-effective': [20], 'microbial': [21], 'processes.': [22], 'As': [23], 'acetate': [24, 63, 76, 94, 132], 'is': [25], 'known': [26], 'be': [28, 134], 'harmful': [29], 'cell': [31], 'growth,': [32], 'P.': [33, 45, 49, 68, 100, 126, 145], 'KT2440': [35, 70, 102], 'screened': [37], 'three': [39], 'strains': [41], '(P.': [42], 'KT2440,': [44, 128], 'NBRC14164,': [47], 'and': [48, 88, 116], 'aeruginosa': [50], 'PH1)': [51], 'as': [52], 'the': [53, 56, 65, 75, 82, 91, 124], 'host': [54], 'with': [55, 123], 'highest': [57], 'tolerance': [58], '10': [60], 'g/L': [61], 'of': [62, 81, 90, 107, 141], 'in': [64], 'medium.': [66], 'Subsequently,': [67], 'by': [73, 143], 'amplifying': [74], 'assimilation': [77], 'pathway,': [78], 'including': [79], 'overexpression': [80], 'acs': [83, 98], '(encoding': [84, 93], 'acetyl-CoA': [85], 'synthetase)': [86], 'route': [87], 'construction': [89], 'ackA-pta': [92], 'kinase-phosphotransacetylase)': [95], 'pathway.': [96], 'The': [97], 'overexpressing': [99], 'showed': [103], 'remarkable': [105], 'increase': [106], 'mcl-PHA': [108, 112, 118, 142], 'titer': [109], '(+': [110, 114, 120], '92%),': [111], 'yield': [113], '50%),': [115], 'cellular': [117], 'content': [119], '43%)': [121], 'compared': [122], 'wild-type': [125], 'which': [129], 'indicated': [130], 'that': [131], 'could': [133], 'potential': [136], 'substrate': [137], 'for': [138], 'biochemical': [139], 'production': [140], 'putida.': [146]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2922252081', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 14}, {'year': 2022, 'cited_by_count': 15}, {'year': 2021, 'cited_by_count': 8}, {'year': 2020, 'cited_by_count': 8}], 'updated_date': '2024-09-11T23:09:58.756996', 'created_date': '2019-03-22'}