Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2912145609', 'doi': 'https://doi.org/10.1101/544247', 'title': 'Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, <i>Gambierdiscus lapillus</i> (Gonyaulacales, Dinophyceae)', 'display_name': 'Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, <i>Gambierdiscus lapillus</i> (Gonyaulacales, Dinophyceae)', 'publication_year': 2019, 'publication_date': '2019-02-08', 'ids': {'openalex': 'https://openalex.org/W2912145609', 'doi': 'https://doi.org/10.1101/544247', 'mag': '2912145609'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/544247', 'pdf_url': 'https://www.biorxiv.org/content/biorxiv/early/2019/02/08/544247.full.pdf', 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, 'type': 'preprint', 'type_crossref': 'posted-content', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://www.biorxiv.org/content/biorxiv/early/2019/02/08/544247.full.pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5055902119', 'display_name': 'Anna Liza Kretzschmar', 'orcid': 'https://orcid.org/0000-0001-6415-3979'}, 'institutions': [{'id': 'https://openalex.org/I114017466', 'display_name': 'University of Technology Sydney', 'ror': 'https://ror.org/03f0f6041', 'country_code': 'AU', 'type': 'education', 'lineage': ['https://openalex.org/I114017466']}], 'countries': ['AU'], 'is_corresponding': True, 'raw_author_name': 'A.L. Kretzschmar', 'raw_affiliation_strings': ['Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia', 'ithree institute (i3), University of Technology Sydney, Ultimo, 007 NSW, Australia,'], 'affiliations': [{'raw_affiliation_string': 'ithree institute (i3), University of Technology Sydney, Ultimo, 007 NSW, Australia,', 'institution_ids': ['https://openalex.org/I114017466']}, {'raw_affiliation_string': 'Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia', 'institution_ids': ['https://openalex.org/I114017466']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5005531676', 'display_name': 'Arjun Verma', 'orcid': 'https://orcid.org/0000-0003-0119-9915'}, 'institutions': [{'id': 'https://openalex.org/I114017466', 'display_name': 'University of Technology Sydney', 'ror': 'https://ror.org/03f0f6041', 'country_code': 'AU', 'type': 'education', 'lineage': ['https://openalex.org/I114017466']}], 'countries': ['AU'], 'is_corresponding': False, 'raw_author_name': 'A. Verma', 'raw_affiliation_strings': ['Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia'], 'affiliations': [{'raw_affiliation_string': 'Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia', 'institution_ids': ['https://openalex.org/I114017466']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5070048995', 'display_name': 'Gurjeet S. Kohli', 'orcid': 'https://orcid.org/0000-0002-4578-2355'}, 'institutions': [{'id': 'https://openalex.org/I114017466', 'display_name': 'University of Technology Sydney', 'ror': 'https://ror.org/03f0f6041', 'country_code': 'AU', 'type': 'education', 'lineage': ['https://openalex.org/I114017466']}, {'id': 'https://openalex.org/I127251866', 'display_name': 'Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung', 'ror': 'https://ror.org/032e6b942', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I127251866', 'https://openalex.org/I1305996414']}], 'countries': ['AU', 'DE'], 'is_corresponding': False, 'raw_author_name': 'G.S. Kohli', 'raw_affiliation_strings': ['Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany', 'Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia'], 'affiliations': [{'raw_affiliation_string': 'Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia', 'institution_ids': ['https://openalex.org/I114017466']}, {'raw_affiliation_string': 'Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany', 'institution_ids': ['https://openalex.org/I127251866']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5077003877', 'display_name': 'Shauna A. Murray', 'orcid': 'https://orcid.org/0000-0001-7096-1307'}, 'institutions': [{'id': 'https://openalex.org/I114017466', 'display_name': 'University of Technology Sydney', 'ror': 'https://ror.org/03f0f6041', 'country_code': 'AU', 'type': 'education', 'lineage': ['https://openalex.org/I114017466']}], 'countries': ['AU'], 'is_corresponding': False, 'raw_author_name': 'S.A. Murray', 'raw_affiliation_strings': ['Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia'], 'affiliations': [{'raw_affiliation_string': 'Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia', 'institution_ids': ['https://openalex.org/I114017466']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': ['https://openalex.org/A5055902119'], 'corresponding_institution_ids': ['https://openalex.org/I114017466'], 'apc_list': None, 'apc_paid': None, 'fwci': None, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.868231, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 77, 'max': 79}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11985', 'display_name': 'Marine Toxins and Detection Methods', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2304', 'display_name': 'Environmental Chemistry'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11985', 'display_name': 'Marine Toxins and Detection Methods', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/2304', 'display_name': 'Environmental Chemistry'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10032', 'display_name': 'Marine and coastal ecosystems', 'score': 0.9886, 'subfield': {'id': 'https://openalex.org/subfields/1910', 'display_name': 'Oceanography'}, 'field': {'id': 'https://openalex.org/fields/19', 'display_name': 'Earth and Planetary Sciences'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10765', 'display_name': 'Marine Biology and Ecology Research', 'score': 0.9731, 'subfield': {'id': 'https://openalex.org/subfields/1910', 'display_name': 'Oceanography'}, 'field': {'id': 'https://openalex.org/fields/19', 'display_name': 'Earth and Planetary Sciences'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/ciguatoxin', 'display_name': 'Ciguatoxin', 'score': 0.9959264}, {'id': 'https://openalex.org/keywords/ciguatera', 'display_name': 'Ciguatera', 'score': 0.9875567}, {'id': 'https://openalex.org/keywords/dinophyceae', 'display_name': 'Dinophyceae', 'score': 0.7655853}, {'id': 'https://openalex.org/keywords/sargassum', 'display_name': 'Sargassum', 'score': 0.67014253}, {'id': 'https://openalex.org/keywords/dinoflagellate', 'display_name': 'Dinoflagellate', 'score': 0.66083765}], 'concepts': [{'id': 'https://openalex.org/C145316831', 'wikidata': 'https://www.wikidata.org/wiki/Q62010324', 'display_name': 'Ciguatoxin', 'level': 3, 'score': 0.9959264}, {'id': 'https://openalex.org/C2779937837', 'wikidata': 'https://www.wikidata.org/wiki/Q181831', 'display_name': 'Ciguatera', 'level': 3, 'score': 0.9875567}, {'id': 'https://openalex.org/C2778908408', 'wikidata': 'https://www.wikidata.org/wiki/Q2567254', 'display_name': 'Dinophyceae', 'level': 4, 'score': 0.7655853}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.6905014}, {'id': 'https://openalex.org/C2778760044', 'wikidata': 'https://www.wikidata.org/wiki/Q865061', 'display_name': 'Sargassum', 'level': 3, 'score': 0.67014253}, {'id': 'https://openalex.org/C2780204098', 'wikidata': 'https://www.wikidata.org/wiki/Q120490', 'display_name': 'Dinoflagellate', 'level': 2, 'score': 0.66083765}, {'id': 'https://openalex.org/C77077793', 'wikidata': 'https://www.wikidata.org/wiki/Q336019', 'display_name': 'Abundance (ecology)', 'level': 2, 'score': 0.49041367}, {'id': 'https://openalex.org/C18903297', 'wikidata': 'https://www.wikidata.org/wiki/Q7150', 'display_name': 'Ecology', 'level': 1, 'score': 0.44797224}, {'id': 'https://openalex.org/C90856448', 'wikidata': 'https://www.wikidata.org/wiki/Q431', 'display_name': 'Zoology', 'level': 1, 'score': 0.4382867}, {'id': 'https://openalex.org/C559758991', 'wikidata': 'https://www.wikidata.org/wiki/Q37868', 'display_name': 'Algae', 'level': 2, 'score': 0.34508634}, {'id': 'https://openalex.org/C505870484', 'wikidata': 'https://www.wikidata.org/wiki/Q180538', 'display_name': 'Fishery', 'level': 1, 'score': 0.2925616}, {'id': 'https://openalex.org/C2909208804', 'wikidata': 'https://www.wikidata.org/wiki/Q127282', 'display_name': 'Fish <Actinopterygii>', 'level': 2, 'score': 0.12281725}, {'id': 'https://openalex.org/C2780892065', 'wikidata': 'https://www.wikidata.org/wiki/Q184755', 'display_name': 'Phytoplankton', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C142796444', 'wikidata': 'https://www.wikidata.org/wiki/Q181394', 'display_name': 'Nutrient', 'level': 2, 'score': 0.0}], 'mesh': [], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/544247', 'pdf_url': 'https://www.biorxiv.org/content/biorxiv/early/2019/02/08/544247.full.pdf', 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0224664', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0224664&type=printable', 'source': None, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1101/544247', 'pdf_url': 'https://www.biorxiv.org/content/biorxiv/early/2019/02/08/544247.full.pdf', 'source': {'id': 'https://openalex.org/S4306402567', 'display_name': 'bioRxiv (Cold Spring Harbor Laboratory)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I2750212522', 'host_organization_name': 'Cold Spring Harbor Laboratory', 'host_organization_lineage': ['https://openalex.org/I2750212522'], 'host_organization_lineage_names': ['Cold Spring Harbor Laboratory'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/14', 'display_name': 'Life below water', 'score': 0.8}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 65, 'referenced_works': ['https://openalex.org/W1555774552', 'https://openalex.org/W1587026990', 'https://openalex.org/W1802025044', 'https://openalex.org/W1878373200', 'https://openalex.org/W1961569641', 'https://openalex.org/W1969259161', 'https://openalex.org/W1974446183', 'https://openalex.org/W1982631432', 'https://openalex.org/W1985577551', 'https://openalex.org/W1991368494', 'https://openalex.org/W1994000298', 'https://openalex.org/W1999830514', 'https://openalex.org/W2002379566', 'https://openalex.org/W2007784082', 'https://openalex.org/W2007939843', 'https://openalex.org/W2014280716', 'https://openalex.org/W2014292583', 'https://openalex.org/W2017497977', 'https://openalex.org/W2024212190', 'https://openalex.org/W2038305316', 'https://openalex.org/W2039286724', 'https://openalex.org/W2040487555', 'https://openalex.org/W2047247142', 'https://openalex.org/W2051652085', 'https://openalex.org/W2054291654', 'https://openalex.org/W2056836204', 'https://openalex.org/W2059851418', 'https://openalex.org/W2070819783', 'https://openalex.org/W2075805975', 'https://openalex.org/W2076677428', 'https://openalex.org/W2083160591', 'https://openalex.org/W2092093407', 'https://openalex.org/W2093376714', 'https://openalex.org/W2098349315', 'https://openalex.org/W2128880918', 'https://openalex.org/W2132926880', 'https://openalex.org/W2136811808', 'https://openalex.org/W2158351953', 'https://openalex.org/W2168903450', 'https://openalex.org/W2171109906', 'https://openalex.org/W2203496694', 'https://openalex.org/W2271049840', 'https://openalex.org/W2276645241', 'https://openalex.org/W2288064195', 'https://openalex.org/W2315982500', 'https://openalex.org/W2325660457', 'https://openalex.org/W2412507203', 'https://openalex.org/W2460891615', 'https://openalex.org/W2469351400', 'https://openalex.org/W2485436017', 'https://openalex.org/W2487698088', 'https://openalex.org/W2547886108', 'https://openalex.org/W2549419246', 'https://openalex.org/W2554677854', 'https://openalex.org/W2606132044', 'https://openalex.org/W2610590061', 'https://openalex.org/W2735356876', 'https://openalex.org/W2736264002', 'https://openalex.org/W2740080910', 'https://openalex.org/W2768146310', 'https://openalex.org/W2778282816', 'https://openalex.org/W2781601423', 'https://openalex.org/W2949228306', 'https://openalex.org/W4230096730', 'https://openalex.org/W4285719527'], 'related_works': ['https://openalex.org/W4246365450', 'https://openalex.org/W3170356201', 'https://openalex.org/W2944105765', 'https://openalex.org/W2912145609', 'https://openalex.org/W2740080910', 'https://openalex.org/W2610590061', 'https://openalex.org/W2469351400', 'https://openalex.org/W2129328301', 'https://openalex.org/W2041048416', 'https://openalex.org/W2007939843'], 'abstract_inverted_index': {'Abstract': [0], 'Ciguatera': [1, 156, 192], 'fish': [2, 157, 193, 230, 273, 310], 'poisoning': [3, 158, 194], 'is': [4, 16, 73, 159, 210], 'an': [5], 'illness': [6], 'contracted': [7, 163], 'through': [8, 39], 'the': [9, 45, 63, 87, 101, 106, 118, 123, 136, 189, 215, 236, 265, 312, 323, 335], 'ingestion': [10], 'of': [11, 31, 35, 47, 68, 91, 105, 140, 171, 175, 183, 217, 235, 255, 269, 271, 278, 292, 325, 331], 'seafood': [12, 166], 'containing': [13], 'ciguatoxins.': [14], 'It': [15], 'prevalent': [17], 'in': [18, 23, 53, 58, 122, 132, 198, 309, 311, 328], 'tropical': [19, 199], 'regions': [20], 'worldwide,': [21, 196], 'including': [22], 'Australia.': [24, 340], 'Ciguatoxins': [25], 'are': [26, 179, 204, 238], 'produced': [27], 'by': [28, 164, 181, 252, 258], 'some': [29], 'species': [30, 37, 48, 142, 182, 291], 'Gambierdiscus.': [32, 191], 'Therefore,': [33], 'screening': [34], 'Gambierdiscus': [36, 71, 92, 294, 296, 326], 'identification': [38], 'quantitative': [40], 'PCR': [41], '(qPCR),': [42], 'along': [43], 'with': [44, 168, 300], 'determination': [46], 'toxicity,': [49], 'can': [50], 'be': [51, 241], 'useful': [52], 'monitoring': [54, 223], 'potential': [55], 'ciguatera': [56, 129, 229, 248, 272], 'risk': [57], 'these': [59, 141], 'regions.': [60], 'In': [61, 76, 280], 'Australia,': [62], 'identity,': [64], 'distribution': [65], 'and': [66, 89, 103, 138, 150, 207, 224, 232, 267, 319], 'abundance': [67, 90], 'ciguatoxin': [69, 307], 'producing': [70], 'spp.': [72], 'largely': [74], 'unknown.': [75], 'this': [77, 208, 281], 'study': [78], 'we': [79, 283], 'developed': [80, 251], 'a': [81, 95, 128, 160, 169, 253, 276, 285, 290, 329], 'rapid': [82], 'qPCR': [83, 107, 286], 'assay': [84, 110, 287, 316], 'to': [85, 134, 212, 240, 263, 288, 306, 321], 'quantify': [86], 'presence': [88, 137, 324], 'lapillus': [93, 297, 327], ',': [94], 'likely': [96], 'ciguatoxic': [97], 'species.': [98], 'We': [99], 'assessed': [100], 'specificity': [102], 'efficiency': [104], 'assay.': [108], 'The': [109, 173], 'was': [111, 250, 317], 'tested': [112], 'on': [113], '25': [114], 'environmental': [115, 332], 'samples': [116, 144, 333], 'from': [117, 145, 188, 334], 'Heron': [119], 'Island': [120, 202], 'reef': [121], 'southern': [124], 'Great': [125, 336], 'Barrier': [126, 337], 'Reef,': [127], 'endemic': [130], 'region,': [131, 339], 'triplicate': [133], 'determine': [135], 'patchiness': [139], 'across': [143], 'Chnoospora': [146], 'sp.,': [147], 'Padina': [148], 'sp.': [149], 'Sargassum': [151], 'macroalgal': [152], 'hosts.': [153], 'Author’s': [154], 'summary': [155], 'human': [161], 'disease': [162, 237], 'ingesting': [165], 'contaminated': [167], 'group': [170, 174, 254], 'neurotoxins.': [172], 'neurotoxins,': [176], 'named': [177], 'ciguatoxins,': [178], 'synthesised': [180], 'single': [184], 'celled': [185], 'marine': [186], 'algae': [187], 'genus': [190], 'occurs': [195], 'particularly': [197], 'nations.': [200], 'Pacific': [201], 'nations': [203], 'disproportionately': [205], 'impacted,': [206], 'impact': [209], 'predicted': [211], 'increase': [213], 'as': [214, 275], 'effects': [216], 'climate': [218], 'change': [219], 'unfold.': [220], 'Few': [221], 'effective': [222], 'mitigation': [225], 'strategies': [226], 'exist': [227], 'for': [228], 'poisoning,': [231, 274], 'reporting': [233], 'rates': [234], 'estimated': [239], 'approximately': [242], '20%': [243], 'at': [244], 'best.': [245], 'A': [246], 'global': [247], 'strategy': [249], 'researchers': [256], 'coordinated': [257], 'UNESCO’s': [259], 'Intergovernmental': [260], 'Oceanographic': [261], 'Commission': [262], 'characterise': [264], 'cause': [266], 'mode': [268], 'action': [270], 'matter': [277], 'urgency.': [279], 'study,': [282], 'designed': [284], 'detect': [289, 322], 'microalgae,': [293], 'lapillus.': [295], 'produces': [298], 'compounds': [299], 'ciguatoxin-like': [301], 'properties,': [302], 'which': [303], 'may': [304], 'lead': [305], 'uptake': [308], 'Australian': [313], 'region.': [314], 'This': [315], 'sensitive': [318], 'able': [320], 'range': [330], 'Reef': [338]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2912145609', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 3}], 'updated_date': '2024-12-16T13:31:20.654886', 'created_date': '2019-02-21'}