Publication Information

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4519], 'T4S': [7, 221, 290, 659, 807, 828, 865, 884, 1216, 3058, 3067, 3118, 3200, 3654, 3680, 3788, 3876, 3892, 3939, 3950, 4007, 4025, 4083, 4177, 4199, 4345, 4397, 4407, 4492], 'systems': [8, 222, 660, 663, 835, 885, 3940, 3951, 3956, 4008, 4036, 4054, 4323, 4362, 4381, 4426, 4430, 4486, 4493, 4496, 4539], 'in': [9, 105, 137, 215, 223, 323, 367, 425, 455, 478, 499, 532, 562, 616, 640, 657, 666, 677, 700, 824, 837, 882, 960, 969, 1051, 1320, 1404, 1455, 1605, 1619, 1631, 1646, 1850, 1916, 2097, 2162, 2173, 2181, 2312, 2317, 2378, 2394, 2457, 2468, 2606, 2614, 2714, 2871, 2930, 2937, 3132, 3165, 3241, 3298, 3316, 3396, 3400, 3478, 3494, 3513, 3540, 3623, 3626, 3663, 3791, 3801, 3806, 3899, 3957, 4019, 4037, 4055, 4093, 4120, 4141, 4155, 4166, 4296, 4355, 4453, 4471, 4575, 4582], 'Gram-negative': [10, 162, 224, 701, 841, 954, 3937, 4038], 'bacteria:': [11], 'a': [12, 138, 142, 146, 171, 182, 189, 196, 240, 311, 315, 320, 341, 409, 432, 538, 545, 586, 596, 620, 681, 709, 715, 726, 738, 762, 775, 779, 917, 983, 988, 995, 1027, 1179, 1204, 1321, 1363, 1477, 1481, 1491, 1670, 1790, 1802, 1828, 1837, 1855, 1870, 1902, 2042, 2049, 2053, 2058, 2115, 2174, 2206, 2216, 2249, 2259, 2295, 2395, 2412, 2469, 2525, 2650, 2770, 2850, 3066, 3072, 3216, 3248, 3306, 3313, 3322, 3344, 3355, 3368, 3379, 3479, 3555, 3636, 3747, 4041, 4068, 4074, 4082, 4153, 4162, 4184, 4194, 4258, 4302, 4316, 4416, 4546, 4573], 'mechanistic': [13, 241, 1120, 2495], 'biology': [14, 378], 'perspective': [15], 'Gabriel': [16, 20, 43, 47, 72], 'Waksman': [17, 21, 44, 48, 73, 4063], 'Corresponding': [18, 45], 'Author': [19, 46, 70], '[email': [22, 49, 94], 'protected]': [23, 50, 95], 'orcid.org/0000-0003-0708-2726': [24, 51], 'Institute': [25, 52], 'of': [26, 53, 76, 110, 151, 156, 166, 218, 343, 353, 355, 376, 386, 396, 419, 458, 464, 475, 486, 489, 540, 566, 619, 623, 683, 734, 748, 764, 782, 813, 816, 832, 840, 848, 901, 909, 919, 924, 953, 982, 1018, 1039, 1108, 1131, 1173, 1200, 1230, 1254, 1269, 1277, 1288, 1317, 1370, 1374, 1422, 1447, 1461, 1471, 1476, 1537, 1603, 1676, 1697, 1711, 1761, 1821, 1860, 1893, 1922, 1941, 1973, 1985, 1989, 1991, 1995, 1997, 2005, 2091, 2194, 2209, 2219, 2258, 2262, 2298, 2307, 2334, 2350, 2359, 2376, 2392, 2407, 2417, 2445, 2454, 2465, 2505, 2509, 2533, 2536, 2580, 2692, 2698, 2702, 2731, 2754, 2761, 2774, 2797, 2823, 2861, 2869, 2892, 2926, 2972, 3010, 3025, 3054, 3079, 3096, 3114, 3151, 3215, 3269, 3291, 3302, 3408, 3443, 3503, 3543, 3557, 3571, 3593, 3618, 3635, 3640, 3644, 3678, 3684, 3691, 3726, 3743, 3751, 3767, 3785, 3890, 3902, 3906, 3920, 3944, 3988, 3990, 4052, 4081, 4106, 4124, 4127, 4129, 4145, 4186, 4197, 4214, 4221, 4232, 4265, 4269, 4281, 4301, 4333, 4365, 4387, 4433, 4437, 4509, 4513, 4558, 4568, 4598, 4628], 'Structural': [27, 54, 77], 'and': [28, 32, 55, 59, 78, 82, 113, 158, 195, 212, 220, 233, 256, 334, 339, 365, 391, 450, 467, 496, 534, 672, 676, 725, 761, 790, 801, 853, 872, 907, 912, 926, 937, 944, 987, 1019, 1045, 1111, 1117, 1133, 1150, 1170, 1186, 1272, 1292, 1297, 1303, 1346, 1419, 1429, 1436, 1444, 1463, 1484, 1490, 1562, 1592, 1616, 1621, 1640, 1728, 1731, 1737, 1777, 1781, 1818, 1854, 1901, 1994, 2012, 2029, 2100, 2128, 2248, 2419, 2551, 2598, 2620, 2670, 2683, 2719, 2768, 2782, 2833, 2845, 2875, 2911, 2963, 2981, 3003, 3022, 3094, 3169, 3176, 3245, 3277, 3287, 3354, 3370, 3399, 3421, 3427, 3433, 3464, 3484, 3489, 3497, 3590, 3606, 3728, 3757, 3819, 3822, 3871, 3887, 3933, 3964, 3971, 3984, 3999, 4058, 4062, 4103, 4122, 4135, 4160, 4235, 4275, 4359, 4449, 4459, 4468, 4499, 4511, 4515, 4526, 4553, 4561, 4571, 4600, 4612], 'Molecular': [29, 56, 79], 'Biology,': [30, 57, 80], 'UCL': [31, 58, 81], 'Birkbeck,': [33, 60, 83], 'London,': [34, 61, 84], 'UK': [35, 62, 85], 'Search': [36, 63], 'for': [37, 64, 102, 558, 1029, 1072, 1348, 1381, 1499, 1779, 1873, 1882, 1961, 1970, 2083, 2191, 2289, 2538, 2561, 2728, 3052, 3075, 3159, 3196, 3255, 3359, 3816, 3828, 4048, 4183, 4391, 4394, 4440], 'more': [38, 65, 1803, 3741, 4030, 4069, 4389, 4421, 4500], 'papers': [39, 66], 'by': [40, 67, 131, 170, 304, 330, 363, 431, 482, 515, 642, 680, 705, 797, 1242, 1300, 1306, 1342, 1387, 1547, 1577, 1771, 1784, 2106, 2114, 2511, 2881, 3338, 3771, 4043, 4060, 4087, 4109, 4204, 4476, 4595], 'this': [41, 68, 106, 476, 1097, 1209, 2131, 2179, 2569, 2755, 3080, 3263, 4170, 4201, 4252], 'author': [42, 69], 'Information': [71], '*,1': [74], '1Institute': [75], '*Corresponding': [86], 'author.': [87], 'Tel:': [88], '+44': [89], '207': [90], '631': [91], '6833;': [92], 'E-mail:': [93], 'EMBO': [96], 'Reports': [97], '(2019)20:e47012https://doi.org/10.15252/embr.201847012': [98], 'See': [99], 'the': [100, 129, 154, 164, 207, 216, 230, 237, 302, 327, 350, 368, 374, 384, 393, 417, 420, 428, 446, 456, 462, 472, 479, 483, 487, 581, 732, 735, 742, 746, 759, 786, 794, 798, 806, 814, 817, 820, 857, 862, 875, 947, 950, 966, 1065, 1103, 1115, 1123, 1129, 1145, 1168, 1174, 1191, 1197, 1201, 1215, 1225, 1231, 1234, 1243, 1247, 1251, 1255, 1260, 1278, 1289, 1301, 1307, 1314, 1328, 1334, 1349, 1371, 1375, 1392, 1399, 1405, 1411, 1416, 1420, 1423, 1433, 1442, 1517, 1522, 1538, 1548, 1553, 1578, 1583, 1617, 1638, 1683, 1690, 1695, 1698, 1743, 1759, 1762, 1772, 1785, 1816, 1822, 1825, 1851, 1858, 1883, 1913, 1939, 1946, 1964, 1971, 1978, 1986, 2002, 2006, 2009, 2037, 2064, 2074, 2086, 2092, 2107, 2120, 2133, 2150, 2155, 2159, 2163, 2182, 2187, 2195, 2210, 2220, 2225, 2232, 2244, 2254, 2263, 2287, 2290, 2299, 2305, 2308, 2313, 2318, 2335, 2344, 2354, 2373, 2446, 2450, 2473, 2503, 2506, 2530, 2540, 2546, 2549, 2552, 2558, 2581, 2584, 2588, 2607, 2615, 2623, 2630, 2634, 2642, 2654, 2659, 2668, 2681, 2690, 2693, 2708, 2735, 2740, 2747, 2750, 2762, 2780, 2792, 2798, 2803, 2811, 2843, 2854, 2866, 2873, 2885, 2889, 2909, 2927, 2931, 2966, 2969, 2973, 2983, 2988, 2993, 3001, 3011, 3020, 3026, 3032, 3039, 3049, 3057, 3076, 3112, 3117, 3124, 3133, 3147, 3157, 3173, 3177, 3187, 3199, 3219, 3256, 3265, 3270, 3279, 3317, 3330, 3336, 3388, 3406, 3409, 3449, 3475, 3501, 3541, 3562, 3615, 3619, 3632, 3641, 3648, 3653, 3671, 3679, 3688, 3733, 3744, 3768, 3777, 3782, 3798, 3809, 3830, 3843, 3849, 3855, 3858, 3862, 3869, 3875, 3891, 3900, 3903, 3918, 3930, 3936, 3942, 4024, 4088, 4101, 4130, 4136, 4142, 4156, 4167, 4176, 4198, 4205, 4228, 4249, 4276, 4285, 4288, 4297, 4337, 4340, 4366, 4373, 4395, 4405, 4424, 4434, 4454, 4463, 4472, 4477, 4507, 4520, 4523, 4543, 4551, 4566, 4569, 4576, 4583, 4593, 4623], 'Glossary': [101, 243], 'abbreviations': [103], 'used': [104, 1868, 2096, 2238], 'article.': [107], 'PDFDownload': [108], 'PDF': [109], 'article': [111], 'text': [112], 'main': [114, 351], 'figures.': [115], 'ToolsAdd': [116], 'favoritesDownload': [118], 'CitationsTrack': [119], 'CitationsPermissions': [120], 'ShareFacebookTwitterLinked': [121], 'InMendeleyWechatReddit': [122], 'Figures': [123], '&': [124], 'Info': [125], 'Abstract': [126], 'Conjugation': [127, 699, 729], 'is': [128, 168, 209, 301, 307, 349, 703, 830, 888, 956, 980, 1208, 1220, 1236, 1257, 1286, 1340, 1474, 1510, 1556, 1566, 1586, 1596, 1612, 1644, 1862, 2112, 2135, 2222, 2266, 2286, 2301, 2522, 2571, 2627, 2638, 2678, 2742, 2759, 2920, 3083, 3155, 3213, 3267, 3321, 3343, 3448, 3520, 3538, 3638, 3650, 3839, 3861, 4028, 4149, 4219, 4245, 4254, 4348, 4372, 4481, 4556, 4590, 4626], 'process': [130, 165, 303, 342, 1322], 'which': [132, 186, 305, 331, 551, 658, 1041, 1061, 1395, 1413, 1441, 1735, 1861, 2154, 2539, 2562, 2954, 3221, 3334, 3867, 3945, 4377, 4631], 'bacteria': [133, 332, 533, 702, 842, 955, 4039, 4057], 'exchange': [134], 'genetic': [135, 387, 520, 541], 'materials': [136], 'unidirectional': [139, 390], 'manner': [140], 'from': [141, 239, 310, 402, 438, 690, 861, 2023, 2158, 2253, 2529, 2859, 3610, 3732, 4284, 4336, 4399, 4409], 'donor': [143, 312, 439, 449, 1044, 2164, 2314], 'cell': [144, 313, 404, 559, 598, 2165, 2184, 2227, 2315, 2320], 'recipient': [147, 316, 451, 587, 1022, 1046, 1261, 2183, 2226, 2319], 'cell.': [148, 317, 1262], 'The': [149, 502, 654, 752, 767, 827, 892, 1137, 1228, 1265, 1283, 1378, 1609, 1625, 1635, 1919, 1931, 2046, 2109, 2577, 2835, 3211, 3300, 3445, 3470, 3787, 3836, 4077, 4531, 4587, 4639], 'discovery': [150, 372, 463], 'signalled': [153, 373], 'dawn': [155, 375], 'genetics': [157], 'molecular': [159, 377, 490, 2003], 'biology.': [160], 'In': [161, 1096, 1665, 2085, 2965, 3262, 3405, 4536], 'bacteria,': [163, 225], 'mediated': [169, 704], 'large': [172, 183, 197, 576, 707, 996, 1658, 3931, 4079], 'membrane-embedded': [173, 716], 'machinery': [174, 191, 711, 718], 'termed': [175, 192, 200, 719, 851, 1181, 1673, 2828, 2906, 3092, 3226], '"conjugative': [176], 'type': [177, 291, 1134, 3844], 'IV': [178, 292, 721, 1135, 3845], 'secretion': [179, 293, 722, 834, 1217, 3846, 3955, 4035, 4053, 4322], '(T4S)': [180, 723], 'system",': [181], 'injection': [184, 4066], 'nanomachine,': [185], 'together': [187, 1048, 3386], 'with': [188, 226, 442, 731, 811, 999, 1513, 1614, 1807, 1909, 1935, 2057, 2137, 2277, 2449, 2662, 2700, 3123, 3347, 3387, 3506, 3527, 3669, 3687, 3737, 3754, 3781, 4494, 4548, 4563], 'DNA-processing': [190, 710], '"the': [193, 713, 3087, 4114], 'relaxosome"': [194], 'extracellular': [198, 4341], 'tube': [199], '"pilus"': [201], 'orchestrates': [202], 'directional': [203], 'DNA': [204, 254, 296, 306, 510, 667, 744, 784, 1325, 1702, 1705, 1849, 2072, 2197, 3308, 3318, 3337, 3477, 3507, 3600, 3831, 3997], 'transfer.': [205], 'Here,': [206, 1748], 'focus': [208, 1155, 1751, 1760, 3927], 'on': [210, 229, 741, 778, 1106, 1128, 1144, 1156, 1534, 1752, 2052, 2070, 2331, 2343, 2353, 2442, 2924, 3031, 3440, 3474, 3614, 3928, 4065, 4261, 4307, 4592], 'past': [211], 'latest': [213], 'research': [214, 477, 1126, 1158], 'field': [217, 238, 488], 'an': [227, 896, 957, 1485, 1808, 1841, 1897, 1954, 2379, 2458, 2485, 2806, 2839, 2903, 2916, 3043, 3348, 3565, 3675, 3693, 3885, 4049, 4262], 'emphasis': [228], 'various': [231, 503, 655, 1116, 1518, 3584, 3620], 'questions': [232], 'debates': [234], 'that': [235, 383, 392, 573, 972, 1067, 1122, 1166, 1213, 1219, 1323, 1681, 1814, 1834, 1847, 2062, 2118, 2271, 2368, 2429, 2462, 2701, 2860, 2900, 2919, 2950, 3191, 3310, 3351, 3535, 3604, 3695, 3750, 3797, 3881, 3935, 4213, 4627], 'permeate': [236], 'perspective.': [242], 'CP': [244], 'coupling': [245, 821, 3088, 3672, 3864], 'protein': [246, 286, 644, 757, 822, 1393, 1401, 1412, 1699, 2047, 3126, 3324, 3346, 3865, 3915, 4004], 'cryo-EM': [247], 'cryo-electron': [248, 251, 2512], 'microscopy': [249, 2513], 'Cryo-ET': [250], 'tomography': [252], 'dtr': [253], 'transfer': [255, 295, 385, 511, 584, 1080, 1318], 'replication': [257, 3824], 'ICE': [258], 'integrated': [259, 525], 'conjugative': [260, 516, 526, 951, 1035, 1056], 'elements': [261, 522, 527], 'IMC': [262, 913, 4263], 'inner-membrane': [263, 897], 'complex': [264, 280, 879, 898, 1611, 2098, 3567, 3770, 3838, 4031, 4171, 4218, 4231, 4241, 4253, 4534], 'IM': [265, 4584], 'inner': [266, 4168], 'membrane': [267, 283, 4158], 'IR': [268, 2068, 2089, 2110, 2721, 3461], 'inverted': [269, 1955], 'repeat': [270, 1956], 'LPS': [271], 'lipopolysaccharides': [272], 'mpf': [273], 'mating': [274, 447, 3810, 3820], 'pair': [275, 3811], 'formation': [276, 2099, 3812], 'OMCC': [277, 893, 911, 4544, 4603], 'outer-membrane': [278, 877, 4238, 4532], 'core': [279, 878, 4115, 4230, 4240, 4533], 'OM': [281, 285, 890, 4577], 'outer': [282, 4157], 'OmpA': [284], 'A': [287, 3420, 4358], 'RHH': [288], 'ribbon-helix-helix': [289, 3331], 'TrIP': [294], 'immuno-precipitation': [297], 'Introduction': [298], 'Bacterial': [299], 'transferred': [308, 437, 1223, 1258], 'unidirectionally': [309], 'It': [318, 359, 979, 1207, 2265, 4226, 4555], 'plays': [319, 769], 'crucial': [321], 'role': [322], 'horizontal': [324], 'gene': [325, 434, 465], 'transfer,': [326], 'major': [328, 984], 'means': [329], 'evolve': [333], 'adapt': [335], 'their': [337, 1794, 3166, 4528], 'environment,': [338], 'also': [340, 591, 1082, 1332, 2501, 3780, 4527, 4579], 'immense': [344], 'biomedical': [345], 'importance': [346], 'since': [347, 4243, 4324], 'vector': [352], 'propagation': [354], 'antibiotics': [356], 'resistance': [357], 'genes.': [358], 'was': [360, 381, 389, 688, 965, 2095, 2566, 3696, 4091, 4117, 4172, 4318, 4412], 'first': [361, 1101, 2474, 4078], 'described': [362, 1526, 2033, 3036, 3063, 4092, 4329], 'Lederberg': [364], 'Tatum': [366], '1940s': [369], '1.': [370, 653], 'Its': [371, 3361], 'once': [379, 1250], 'it': [380, 773, 803, 964, 3794, 3873, 3879, 3898, 3908, 4244, 4347, 4419], 'demonstrated': [382, 1240, 2428, 3716], 'information': [388], 'entire': [394, 2660], 'genome': [395, 423], 'Escherichia': [397, 4146], 'coli': [398, 422, 4147], 'could': [399, 973, 2236, 2542, 2957, 3803], 'be': [400, 436, 974, 1222, 1717, 1867, 2032, 2103, 2149, 2237, 2490, 2543, 2977, 3062, 3252, 3628, 3804, 3884, 4488], 'passed': [401], 'one': [403, 755, 812, 831, 1533, 2088, 2283, 2345, 2441, 2760, 3639, 4020], 'another': [406, 4059], 'starting': [407], 'at': [408, 785, 1002, 1178, 1313, 1689, 1694, 1793, 2256, 3145, 3832], 'defined': [410, 536, 3480], 'site': [411, 740, 789, 1180, 1336, 1339, 1418, 1424, 1555, 1585, 1948, 1980, 2016, 2122, 2212, 2337, 2415, 2971, 3382, 3446, 3468], '2.': [412, 1264], 'Indeed,': [413, 1054, 2757, 2817], 'landmark': [414], 'discoveries': [415], 'followed:': [416], 'mapping': [418], 'E.': [421], '(mapped': [424], '"minutes",': [426], 'i.e.': [427], 'time': [429, 443, 4202], 'taken': [430], 'particular': [433, 739], 'recipient,': [441], '0': [444], 'being': [445, 2382, 2398, 2456, 2467, 2933, 3564, 3958, 4012, 4497, 4580], 'start—when': [448], 'cells': [452, 605, 971, 1023, 1047, 1086], 'were': [453, 3103, 3130, 4327], 'put': [454], 'presence': [457, 3542], 'each': [459, 1107, 1361, 1382, 1409, 1514, 1535, 2443, 2820, 2938, 3441, 3528, 3611, 3912, 4268], 'other)': [460], 'or': [461, 518, 603, 632, 750, 929, 939, 976, 1024, 1032, 1352, 1704, 1708, 2316, 2575, 3015, 3149, 4462], 'structure': [466, 1460, 1470, 1806, 2504, 2521, 2541, 2582, 2617, 2691, 3301, 3634, 4108, 4260], 'regulation': [468], '(please': [469], 'refer': [470], 'fascinating': [473], 'account': [474], 'Nobel': [480], 'lectures': [481], 'founding': [484], 'fathers': [485], 'biology,': [491], 'Francois': [492], 'Jacob,': [493], 'Andre': [494], 'Lwoff': [495], 'Jacques': [497], 'Monod': [498], '1965': [500], '3).': [501], 'machineries': [504], 'utilized': [505], 'during': [506, 669], 'execute': [509], 'are': [512, 530, 535, 610, 661, 664, 846, 914, 1037, 1070, 1087, 1164, 1385, 1425, 1497, 1627, 1653, 1881, 2275, 2583, 2603, 2611, 2723, 2879, 2894, 2944, 3142, 3194, 3374, 3415, 3596, 3668, 3765, 3779, 3852, 3952, 4009, 4382], 'usually': [513, 547, 554, 1654, 3156], 'encoded': [514, 1299, 1305, 1770, 1783, 4086, 4203, 4475], 'plasmids': [517, 1774], 'other': [519, 3612, 4034], 'mobile': [521], 'such': [523, 599, 626, 2649], 'as': [524, 537, 600, 627, 1013, 1026, 1525, 1630, 1755, 1836, 1869, 1959, 2041, 2205, 2285, 2488, 2815, 3071, 3086, 3107, 3154, 3434, 3776, 3842, 3854, 3977, 3996, 4003, 4011, 4330, 4415, 4490], '(ICE).': [528], 'Plasmids': [529], 'ubiquitous': [531], 'collection': [539], 'modules': [542, 568, 609], 'organized': [543], 'into': [544, 575, 646, 994, 1719, 2224, 2242], 'stable,': [546], 'circular,': [548], 'self-replicating': [549], 'replicon,': [550], 'does': [552], 'not': [553, 1656, 2272, 2557, 2612, 3017, 3143, 3714], 'contain': [555, 569, 1669], 'genes': [556, 570, 3799, 3814, 3826], 'essential': [557, 638, 770, 958, 2188], 'functions': [560, 1910, 4018], '(reviewed': [561, 823, 3240, 3512, 3662], 'ref.': [563, 825, 3397, 3664], '4).': [564], 'Several': [565], 'these': [567, 608, 1109, 2941, 2955, 3139, 3152, 3182, 3192, 3238, 3504, 3524, 3550, 3572, 3594, 3708, 4056], 'encoding': [571], 'proteins': [572, 850, 1311, 1660, 1800, 3153, 3210, 3228, 3239, 3385, 3472, 3505, 3525, 3551, 3573, 3595, 3607, 3721, 3756, 3963, 4369, 4436], 'assemble': [574, 1312, 3473], 'complexes': [577, 1110, 3860, 4597], 'mediating': [578, 1016, 3110], 'most': [579, 1124, 1763, 2763, 2802, 3160, 4432, 4537], 'commonly': [580], "plasmid's": [582, 1315], 'own': [583], 'bacterial': [588, 624, 684, 1308, 3938, 4321], 'cell,': [589], 'but': [590, 1796, 1865, 2388, 2556, 3164, 3578, 3706, 4251], 'intriguingly': [592], '(but': [593, 1655], 'rarely)': [594], 'eukaryotic': [597, 647], 'yeast,': [601], 'plant': [602], 'human': [604], '5-7.': [606], 'Interestingly,': [607, 2687, 2936, 4470], 'evolutionary': [611], 'related': [612, 3328, 4422], 'clusters': [614], 'found': [615], 'genomic': [617], 'islands': [618], 'restricted': [621], 'number': [622, 682, 763, 4185], 'pathogens': [625], 'Helicobacter': [628], 'pylori,': [629], 'Bordetella': [630], 'pertussis': [631], 'Legionella': [633, 3752], 'pneumophila': [634, 4401], 'where': [635, 886], 'they': [636, 845, 1396, 1668, 1756, 2610, 3046, 3967, 3975, 3994, 4001], 'play': [637], 'roles': [639], 'pathogenicity': [641], 'injecting': [643], 'effectors': [645], 'hosts': [648], '8': [649], '(Fig': [650, 1183, 1661, 2437, 2518, 2618, 3229, 4585], '1).': [651], 'Figure': [652, 1263, 4637], 'processes': [656], 'involvedT4S': [662], 'involved': [665, 3800], 'transport': [668, 679, 717, 818, 1226, 1229, 3961, 3970], 'conjugation,': [670], 'transformation': [671], 'A.': [673, 4607], 'tumefaciens': [674, 864], 'infection,': [675], 'effector': [678], 'pathogens.': [685], 'This': [686, 1338, 1686, 2019, 2200, 2520, 4111, 4180, 4216, 4299], 'figure': [687, 696, 1649], 'modified': [689], 'Grohmann': [691], 'et': [692, 2363, 2425, 2498, 3773, 4045, 4097, 4190], 'al': [693, 2364, 2426, 2499, 3774, 4046, 4098, 4191], '108.': [694], 'Download': [695, 697, 1648, 1650], 'PowerPoint': [698, 1651], 'three': [706, 941, 1293, 2589, 3429, 4017, 4128, 4443], 'complexes:': [708], 'called': [712, 745, 758], 'relaxosome";': [714], '"type': [720], 'system";': [724], 'pilus': [727, 952, 3817, 3978, 3991], '9.': [728], 'starts': [730], 'assembly': [733, 3972], 'relaxosome': [736, 753, 1138, 1270, 1285, 1383, 1874, 3259, 3266, 3471, 3563, 3637, 3649, 3870], 'plasmid': [743, 1175, 1280, 1302, 1501, 1798, 1885, 1992, 1999, 2196, 2293, 3098, 3258, 3411, 3735], '"origin': [747], 'transfer"': [749], 'OriT.': [751, 1466, 1508, 3288], 'includes': [754], 'key': [756], '"relaxase"': [760], 'accessory': [765, 1294, 3260, 3544, 3720, 3755], 'proteins.': [766, 3261], 'relaxase': [768, 1160, 1232, 1290, 1826, 2300, 2984, 3027, 3136, 3212, 3271, 3389, 3536], 'roles:': [771], '(i)': [772, 2411, 3569, 3974], 'catalyses': [774, 1815], 'nicking': [776, 799, 1817], 'reaction': [777, 2161, 2177, 2202], 'single': [780, 1903, 2694], 'strand': [781], 'OriT': [783, 1176, 1329, 1331, 1372, 1942, 1974, 2430, 3394, 3407, 3476, 3624, 3833], 'so-called': [787, 876], 'nic': [788, 1439, 1539, 1554, 1584, 1947, 1979, 2211, 2257, 2336, 2377, 2393, 2418, 2447, 2455, 2466, 3463], 'covalently': [791, 1194, 2167], 'reacts': [792], '5′-phosphate': [795, 2156], 'generated': [796], 'reaction;': [800], '(ii)': [802, 2420, 3591, 3993], 'binds': [804, 1414, 2372, 2389, 2656, 3352], 'system': [808, 829, 3068, 3655, 3681, 3789, 3847, 3893, 4026, 4084, 4178, 4207, 4209, 4376, 4398, 4408, 4456, 4465, 4474], 'through': [809, 916, 1203, 1224, 1558, 1588, 3121], 'interactions': [810, 3122, 3502, 3548, 3667, 3778], 'constituents': [815], 'machinery,': [819], '10).': [826], 'six': [833], 'embedded': [836, 4140], 'both': [838, 2790, 2848, 2872, 3962], 'membranes': [839, 4144], '11.': [843], 'Minimally,': [844], 'composed': [847, 900, 1287, 1475, 4123, 4220], '12': [849, 4266, 4367], '"VirB1-11': [852], 'VirD4"': [854], '(to': [855], 'use': [856, 2406], 'naming': [858], 'nomenclature': [859], 'derived': [860, 2528], 'Agrobacterium': [863], 'system)': [866], '12.': [867], 'Three': [868], 'components,': [869, 4224], 'VirB7,': [870, 4233, 4559], 'VirB9': [871, 4234, 4560], 'VirB10,': [873, 4562], 'form': [874, 1953, 2077, 2397, 2508, 2902, 2915, 2951, 3367, 4175], '(OMCC),': [880], 'absent': [881], 'Gram-positive': [883], 'there': [887, 3414], 'no': [889, 2563], '13.': [891], 'connect': [894], '(IMC)': [899], 'VirD4,': [902, 3065, 3670], 'VirB4,': [903, 4271, 4447], 'VirB3,': [904, 4270], 'VirB6,': [905, 4273], 'VirB8': [906, 4274], 'part': [908, 3214, 3677, 3889], 'VirB10.': [910], 'connected': [915], 'stalk': [918, 4259], 'unknown': [920, 1712], 'composition,': [921], 'perhaps': [922, 1000, 3120], 'made': [923, 981, 3987, 4264, 4557], 'VirB2': [925, 992], 'VirB5': [927, 1001], '14': [928, 4125, 4192, 4277], 'VirB10': [930, 4134, 4236, 4282, 4564, 4588], '14-16.': [931], 'At': [932, 2130], 'least': [933], 'two': [934, 1448, 1895, 2124, 2138, 2233, 2278, 2328, 2434, 2479, 2736, 2947, 2960, 3273, 3292, 3371, 3416, 3423, 3437, 3702, 3807, 3859, 4143, 4292, 4308, 4311, 4356, 4524, 4549], 'ATPases': [935], '(VirB4': [936], 'VirD4),': [938], 'sometimes': [940, 1118], '(VirB4,': [942], 'VirD4': [943, 3082, 3711, 3748, 3863, 3882, 4448], 'VirB11),': [945], 'power': [946], 'system.': [948, 3059, 3877], 'Finally,': [949, 3038, 3719], 'element': [959], 'conjugation.': [961], 'For': [962], 'decades,': [963], 'only': [967, 1154, 1894, 2031, 2282, 3247, 4624], 'feature': [968], 'conjugating': [970], 'observed': [975, 1060, 1084, 2921, 4255], 'purified': [977], '17.': [978], 'component,': [985], 'VirB2,': [986], 'minor': [989], 'one,': [990], 'VirB5.': [991], 'assembles': [993], 'helical': [997], 'filament': [998], 'its': [1003, 1559, 1563, 1589, 1593, 1606, 2013, 2663, 2675, 3486, 3495, 4107], 'tip': [1004], '18.': [1005], 'Pili': [1006], 'have': [1007, 1058, 1062, 1141, 1741, 1757, 1801, 1927, 2000, 2073, 2366, 2786, 3042, 3533, 3574, 3759, 3946, 4451], 'been': [1008, 1059, 1083, 1142, 1742, 1758, 2026, 2146, 2171, 3235, 3509, 3659, 3715, 3760, 3917, 3947], 'hypothesized': [1009], 'either': [1011, 2311, 2332, 2572, 3146], 'serve': [1012, 1025, 3106], 'attachment': [1014, 1020, 1820], 'devices': [1015], 'recognition': [1017], 'conduit': [1028], 'relaxase/ssDNA': [1030], 'transport,': [1031], 'both.': [1033], 'Some': [1034], 'pili': [1036, 3986], 'capable': [1038], 'retraction,': [1040], 'will': [1042, 1100, 1113, 1152, 1750, 2030, 2489, 3061, 3251, 3896, 3926, 4505], 'bring': [1043, 2241], '19': [1049], 'resulting': [1050, 2157, 2252, 2929], 'close': [1052], 'proximity.': [1053, 3630], 'tight': [1055], 'junctions': [1057], 'led': [1063], 'suggestion': [1066], 'cell-to-cell': [1068], 'contacts': [1069], 'required': [1071, 2190, 2302, 3375], 'take': [1075], 'place': [1076], '20,': [1077], '21.': [1078], 'However,': [1079, 2853, 3530], 'has': [1081, 2025, 2145, 2170, 2493, 3234, 3508, 3658, 3713, 3916, 4161], 'when': [1085, 1358, 2215, 2688, 3203, 4095], 'some': [1088, 3570, 3579, 3592], 'distance': [1089], 'apart': [1090], '(see': [1091, 3717, 4040], 'detailed': [1092], 'discussion': [1093], 'below)': [1094, 1551, 2323], '22.': [1095], 'review,': [1098], 'I': [1099, 1151, 1749, 3895, 3925, 4504], 'describe': [1102], 'up-to-date': [1104], 'knowledge': [1105], 'then': [1112], 'discuss': [1114], 'contradictory': [1119], 'insights': [1121], 'recent': [1125, 1157, 1764], 'shed': [1127], 'mechanisms': [1130], 'secretion.': [1136], 'Excellent': [1139], 'reviews': [1140, 3922], 'written': [1143, 3236], 'subject': [1146, 3919], '10,': [1147, 3243, 3515], '23,': [1148, 1746], '24': [1149], 'here': [1153, 1880, 2035, 3254], 'illuminating': [1159], 'mechanism.': [1161], 'Relaxases': [1162, 1163, 1652, 1715], 'phosphodiesterases': [1165], 'catalyse': [1167, 2304], 'site-': [1169], 'strand-specific': [1171], 'cleavage': [1172, 2176, 2255, 2414], 'region': [1177, 1373, 1434, 1940, 1972, 3180], '"nic"': [1182], '2A,': [1184, 3230, 3402], 'upper': [1185, 1406, 1632, 1663, 1889, 3231], 'lower': [1187, 2439, 3403], 'panels).': [1188], 'Upon': [1189], 'cleavage,': [1190], 'enzyme': [1192], 'remains': [1193], 'attached': [1195], '5′': [1198, 1351, 1435, 1545, 1944, 2349, 2375, 2453, 2664], 'end': [1199], 'T-strand': [1202, 1823, 2221], 'phosphotyrosyl': [1205], 'linkage.': [1206], 'covalent': [1210, 1819], 'ssDNA–protein': [1211], 'conjugate/complex': [1212], 'constitutes': [1214], 'substrate': [1218], 'machinery.': [1227], 'alongside': [1233], 'T-DNA': [1235, 1249, 1256], 'rationalized': [1237], '(and': [1238], 'subsequently': [1239], '25)': [1241], 'requirement': [1244], 'recircularize': [1246], 'single-strand': [1248], 'complete': [1252], 'copy': [1253, 2208, 2218, 2261, 2297], 'relaxosome(A)': [1266], 'Schematic': [1267, 1368], 'diagram': [1268], 'composition': [1271], 'assembly.': [1273], 'Upper': [1274, 1467], 'panel:': [1275, 1367, 1468, 1504], 'Composition': [1276], 'F': [1279, 1376, 1998, 2292, 3257, 3341, 3410, 3685, 4478], 'family': [1281, 1739, 2800, 2808], 'relaxosome.': [1282, 3077, 3856], 'F-family': [1284, 1773, 1797, 1917, 1962, 2370, 3101, 4374], 'TraI': [1291, 1364, 1450, 1462, 1473, 1505, 1509, 1519, 1529, 1541, 1571, 1604, 1615, 1769, 1799, 1996, 2291, 2371, 2435, 2451, 2480, 2510, 2537, 2703, 2758, 2824, 2893, 3055, 3099, 3115, 3465, 3700], 'proteins:': [1295], 'TraY': [1296, 3320], 'TraM': [1298, 3276, 3342, 3372, 3683], 'IHF': [1304, 3281, 3289, 3303, 3311, 3453, 3458], 'genome.': [1309], 'All': [1310, 3666], 'origin': [1316], '(OriT)': [1319], 'affects': [1324], 'topology': [1326, 3487], 'around': [1327], 'region.': [1330], 'contains': [1333, 2825, 3222, 4227, 4378], 'nick': [1335], '(nic).': [1337], 'flanked': [1341], 'regions': [1343, 1350, 3193], '(in': [1344, 2592, 2596, 2601], 'orange': [1345, 1549], 'yellow': [1347, 1579], '3′': [1353, 1437, 1575, 1976, 2358, 2391, 2464, 2532, 2676], 'nic,': [1355, 2351], 'respectively)': [1356, 4461], 'that,': [1357, 2477, 2713], 'single-stranded,': [1359], 'would': [1360, 2166, 2203, 2976, 2985, 2996, 3005], 'bind': [1362, 1397, 1531, 1934, 2433, 3378, 3390, 3710], 'molecule.': [1365], 'Lower': [1366, 1503], 'representation': [1369], 'plasmid.': [1377, 1787], 'binding': [1379, 1464, 1506, 2329, 2340, 2348, 2357, 2718, 2722, 3197, 3588, 3621], 'sites': [1380, 2330, 2737, 3392, 3418, 3425, 3431, 3439, 3622], 'components': [1384, 1875, 3851], 'depicted': [1386, 1417], 'boxes': [1388], 'coloured': [1389, 1628], 'according': [1390], 'using': [1398, 1521], 'same': [1400, 1523, 2812, 4229], 'colour-coding': [1402, 1524], 'shown': [1403, 1569, 1599, 1613, 1645, 1960, 2101, 2147, 2367, 3534], 'panel.': [1407], 'Under': [1408], 'box,': [1410], 'name': [1421], 'indicated.': [1426], '"TraI': [1427, 1430], 'A"': [1428], 'B"': [1431], 'indicate': [1432], 'trans-esterase': [1443, 1478, 1560, 1639, 1671, 1810, 1899, 1932, 1987, 2010, 2039, 2347, 2547, 2631, 2669, 2696], 'helicase': [1445, 1487, 1590, 1642, 1703, 1791, 1830, 1843, 1905, 1965, 2356, 2554, 2595, 2600, 2625, 2636, 2672, 2685, 2784, 2794, 2809, 2821, 2877, 2939, 2974, 3012, 3466], 'domains': [1446, 1520, 1591, 1626, 1693, 1915, 1921, 1933, 1966, 2590, 2647, 2785, 2847, 2975, 3013, 3041], 'individual': [1449, 3913], 'molecules': [1451, 1530, 2436, 2481], 'bind,': [1452], 'respectively': [1453], '(depicted': [1454], 'Fig': [1456, 1887, 3401], '2B).': [1457], '(B)': [1458], 'Domain': [1459, 1469], 'TraI.': [1472, 1502, 2816], 'domain': [1479, 1493, 1561, 1643, 1675, 1687, 1710, 1792, 1805, 1811, 1831, 1839, 1844, 1900, 1907, 1988, 2011, 2040, 2560, 2570, 2626, 2637, 2651, 2697, 2822, 2837, 3350, 3357, 3364], '(orange),': [1480], 'vestigial': [1482, 1641, 1829, 2550, 2594, 2635, 2684, 2781, 2874], '(green)': [1483], 'active': [1486, 1842, 2065, 2121, 2553, 2599, 2624, 2671, 2682, 2783, 2876], '(blue)': [1488], 'domain,': [1489, 1857, 2548, 2632, 2940], 'C-terminal': [1492, 1856, 1906, 2559, 3356, 3689, 3783], '(grey).': [1494], 'Boundary': [1495], 'residues': [1496], 'indicated': [1498, 1511], 'R1': [1500, 1776, 1884, 3097], 'schematically': [1512], 'volume': [1515], 'indicating': [1516, 3546], 'above.': [1527, 3037], 'Two': [1528, 3090], 'OriT,': [1532, 2484], 'side': [1536, 2333, 2444, 3442], 'site.': [1540, 2066], 'bound': [1542, 1557, 1572, 1587, 2452, 2463, 2523, 2666, 2679, 2705, 3204, 3304], 'sequence': [1544, 1574, 1968, 2531, 2882, 3447], '(indicated': [1546, 1576], 'strip': [1550, 1580], 'overall': [1564, 1594], 'conformation': [1565, 1595, 2381], 'open': [1567, 2380, 2459, 2962, 2967, 3021], '(not': [1568, 1598], 'here).': [1570, 1600], 'above)': [1581], 'closed': [1597, 2396, 2470, 2507, 3023], '(C)': [1601], 'Structure': [1602], 'helicase-loaded': [1607], 'mode.': [1608], 'TraI–ssDNA': [1610], 'ssDNA': [1618, 2116, 2134, 2374, 2390, 2655, 2717, 2741, 2932, 2989, 3002, 3206], 'ribbon': [1620], 'stick': [1622], 'representation,': [1623], 'respectively.': [1624], 'coded': [1629], 'panel': [1633], '(B).': [1634], 'linker': [1636, 2948], 'between': [1637, 2008, 2646, 2734, 2959, 3549, 3746, 4522], 'grey.': [1647], 'always)': [1657], 'multidomain': [1659], '2B,': [1662, 1888, 2438], 'panel).': [1664, 1890, 3232, 3404], 'all': [1666, 2273, 4016, 4325, 4442], 'cases,': [1667], '(also': [1672], '"relaxase")': [1674], 'about': [1677, 3237, 3295, 3522], '300': [1678], 'amino': [1679], 'acids': [1680], 'executes': [1682], 'phosphodiesterase': [1684], 'reaction.': [1685], 'locates': [1688], 'N-terminus.': [1691], 'Additional': [1692], 'C-terminus': [1696, 1795], 'may': [1700, 3014, 3016, 3047], 'support': [1701], 'primase': [1706], 'activities,': [1707], 'extra': [1709], 'function': [1713, 1859, 3976], '26.': [1714], 'can': [1716, 2431, 2482, 4487], 'classified': [1718], 'eight': [1720, 4222], '"MOB"': [1721], 'families,': [1722], 'MOBF,': [1723], 'MOBH,': [1724], 'MOBQ,': [1725], 'MOBC,': [1726], 'MOBP': [1727, 1738], 'MOBV,': [1729], 'MOBT': [1730], 'MOBB': [1732], '27,': [1733], 'among': [1734, 3954, 4320], 'MOBF': [1736, 1753, 1766, 1923], 'relaxases': [1740, 1754, 1767, 1924, 2274], 'best': [1744, 4328], 'studied': [1745, 3511], '24.': [1747], 'research.': [1765, 3646], 'include': [1768, 1789, 3897, 4431], '(F,': [1775], 'pED208': [1778], 'example)': [1780], 'TrwC': [1782, 1891, 1990, 2038, 2699, 3135, 3729], 'R388': [1786, 1993, 3134, 3734, 4206], 'Both': [1788], 'extensive': [1804, 4050], 'N-terminal': [1809, 1898, 2829, 3349, 3362, 4278], '(residues': [1812, 1832, 1845], '1–306)': [1813], '28,': [1827, 2017], '315–828)': [1833], 'operates': [1835], 'ssDNA-binding': [1838, 2015, 2970], '29,': [1840, 1981], '864–1,461)': [1846], 'unwinds': [1848], '5′-to-3′': [1852, 2813], 'direction,': [1853], 'still': [1863], 'unclear': [1864], 'might': [1866, 3625, 3883], 'recruitment': [1871, 3053, 3073, 3113], 'platform': [1872, 3074], '30,': [1876], '31': [1877], '(residue': [1878], 'numbers': [1879], 'TraI;': [1886], 'consists': [1892, 3290], 'domains,': [1896, 2555, 2673, 2878], '(active)': [1904], '32': [1908], 'similar': [1911, 2788], 'corresponding': [1914], 'relaxases.': [1918], 'functional': [1920], 'appear': [1925, 3581], 'different': [1928], 'DNA-binding': [1929, 3363, 3381], 'requirements.': [1930], 'high': [1936, 2752], 'affinity': [1937], 'immediately': [1943, 1975], 'containing': [1949, 2123, 2410, 2897], 'sequences': [1950, 2899, 2949, 3129, 3709], 'likely': [1951, 2081], '(IR),': [1957], 'while,': [1958], 'relaxases,': [1963], 'display': [1967], 'specificity': [1969], '33.': [1982], 'Crystal': [1983], 'structures': [1984, 3943, 4508, 4604], 'provided': [2001], 'basis': [2004], 'interaction': [2007, 3694, 3745], 'IR-containing': [2014], '34-36.': [2018], 'work': [2020], 'dating': [2021], 'back': [2022], '2003': [2024], 'reviewed': [2027], 'extensively': [2028, 3510, 3661], 'briefly': [2034], 'taking': [2036], 'model': [2043], '36,': [2044], '37.': [2045], 'displays': [2048], 'fold': [2050], 'built': [2051], 'two-layer': [2054], 'alpha/beta': [2055], 'sandwich,': [2056], 'deep': [2059], 'narrow': [2060], 'cleft': [2061], 'forms': [2063, 2838, 4291, 4545], 'Typically,': [2067], 'repeats': [2069], 'double-stranded': [2071, 3307, 3498], 'potential': [2075, 2139, 3724], 'extruded': [2078, 2093], 'cruciform': [2079, 2094], 'structures,': [2080, 2789], 'important': [2082, 2268], 'binding.': [2084], 'structure,': [2087, 2661, 2748], 'arm': [2090, 2111], 'firmly': [2104], 'embraced': [2105], 'protein.': [2108], 'followed': [2113], 'segment': [2117], 'enters': [2119], 'catalytic': [2125, 2151, 2279], 'tyrosines,': [2126], 'Tyr18': [2127], 'Tyr26.': [2129], 'point,': [2132], 'presented': [2136], 'exit': [2140, 2234], 'paths': [2141, 2235], '36.': [2142], 'Tyr': [2143], '18': [2144], 'residue': [2152], 'onto': [2153, 2946, 2953, 3000], 'nicking/cleavage': [2160], 'react;': [2168], 'Tyr26': [2169], 'implicated': [2172], 'second': [2175, 2201, 2207, 2217, 2260, 2296], 'occurring,': [2178], 'time,': [2180], 'create': [2186], '3′-OH': [2189, 2251, 2310], 'end-joining': [2192], 'recircularization': [2193], '38,': [2198], '39.': [2199], 'occur': [2204], 'inevitably': [2213], 'appears': [2214], '"pushed"': [2223], '(more': [2228, 2321], 'details': [2229, 2322, 3742, 3764], 'below).': [2230], 'Thus,': [2231, 2908, 4483], 'simultaneously': [2239, 2432], 'proximity': [2243], 'Y18': [2245], 'hydroxyl-5′-phosphate': [2246], 'adduct': [2247], 'free': [2250, 2309], 'T-strand.': [2264], 'however': [2267], 'note': [2270], 'endowed': [2276], 'tyrosines.': [2280], 'When': [2281, 4343], 'exists,': [2284], 'case': [2288, 3158], 'relaxase,': [2294], 'production': [2306], '40.': [2324], 'As': [2325, 3060, 3554, 3878], 'mentioned': [2326, 3435], 'above,': [2327, 3436], 'provide': [2338, 3048], 'selective': [2339], 'platforms': [2341], 'for,': [2342], 'hand,': [2346], 'and,': [2352], 'other,': [2355], 'nic.': [2360, 2534, 3444], 'Recently,': [2361, 3740], 'Ilangovan': [2362, 2424, 2497], '41': [2365, 2427, 2500], 'full-length': [2369, 2704], 'susceptible': [2383], 'rapid': [2385], 'proteolysis': [2386], 'degradation,': [2387], 'resistant': [2399], 'proteolytic': [2401], 'degradation.': [2402], 'Moreover,': [2403], 'making': [2404, 4152], 'elegant': [2405], 'OriT-derived': [2408], 'oligonucleotides': [2409], 'photoactivatable': [2413], 'instead': [2416, 3905], 'judiciously': [2421], 'positioned': [2422, 2628], 'fluorophores,': [2423], 'panel),': [2440], 'site,': [2448], 'conformation,': [2460, 2471], 'while': [2461, 2587, 2674, 2842, 3848, 4578], 'providing': [2472], 'experimental': [2475], 'evidence': [2476], 'indeed,': [2478], 'co-occupy': [2483], 'observation': [2486], 'which,': [2487], 'explained': [2491], 'below,': [2492, 3064], 'profound': [2494], 'implications.': [2496], 'determined': [2502], '(cryo-EM)': [2514], 'atomic': [2516], 'resolution': [2517], '2C).': [2519], '22-mer': [2526], 'oligonucleotide': [2527], 'Regions': [2535], 'obtained': [2544], 'included': [2545], 'electron': [2564], 'density': [2565], 'observed,': [2567], 'suggesting': [2568, 2712, 3190], 'very': [2573, 2787, 3518], 'flexible': [2574], 'disordered.': [2576], 'salient': [2578], 'features': [2579], 'following.': [2585], 'Firstly,': [2586], '(trans-esterase': [2591], 'orange),': [2593], 'green)': [2597], 'blue))': [2602], 'linearly': [2604], 'arranged': [2605], 'primary': [2608], 'sequence,': [2609], 'adjacent': [2613], 'three-dimensional': [2616, 3633], '2B': [2619, 2855, 2867, 2890, 2910], 'C);': [2621], 'instead,': [2622], 'near': [2629], 'whereas': [2633], 'distal': [2639], 'relative': [2640], 'latter.': [2643], 'Long': [2644], 'linkers': [2645], 'facilitate': [2648], 'configuration.': [2652], 'Secondly,': [2653], 'longitudinally': [2657], 'across': [2658], 'half': [2665, 2677], 'domain.': [2686], 'superimposing': [2689], 'IR-bound': [2695], 'ssDNA,': [2707, 2928], 'DNAs': [2709, 3605], 'sterically': [2710], 'clash,': [2711], 'TraI,': [2715, 2870, 3272], 'helicase-associated': [2716], 'trans-esterase-associated': [2720], 'mutually': [2724], 'exclusive,': [2725], 'possibly': [2726], 'accounting': [2727], 'earlier': [2729], 'observations': [2730], 'negative': [2732], 'cooperativity': [2733], '29.': [2738], 'Thirdly,': [2739], 'almost': [2743], 'completely': [2744], 'buried': [2745], 'within': [2746, 2884, 3181, 3393, 3629], 'explaining': [2749], 'remarkably': [2751], 'processivity': [2753], 'enzyme.': [2756], 'processive': [2764], 'monomeric': [2765], 'helicases': [2766], 'known': [2767, 3085, 3161, 3521, 3841, 3853], 'exhibits': [2769, 3701, 4632], 'fast': [2771], 'unwinding': [2772, 3004], 'rate': [2773], '~1,100': [2775], 'bp/s': [2776], '42,': [2777], '43.': [2778], 'Fourthly,': [2779], 'exhibiting': [2791, 2810], 'classical': [2793], 'sub-domain': [2795, 2856, 2905], 'organization': [2796, 3617], 'SF1A/B': [2799], 'resembling': [2801], 'RecD2': [2804], 'helicase,': [2805], 'SF1B': [2807], 'directionality': [2814], 'like': [2818], 'RecD2,': [2819, 2865], 'four': [2826], 'sub-domains,': [2827], '(N-term),': [2830], '1A,': [2831], '2A': [2832, 2846, 2886], '2B.': [2834], '"N-term"': [2836], 'α-helical': [2840], 'bundle': [2841], '1A': [2844], 'exhibit': [2849, 4615], 'RecA-like': [2851], 'fold.': [2852], 'differs': [2857], 'markedly': [2858], 'RecD2.': [2862], 'Similarly': [2863], 'sub-domains': [2868, 2891, 2914, 2943, 2956, 2995, 3175, 3183], 'formed': [2880, 3268], 'insertions': [2883], 'domain;': [2887], 'however,': [2888], 'much': [2895, 4384], 'larger,': [2896, 4385], 'additional': [2898, 2904, 3044, 3224], 'themselves': [2901], '"2B-like".': [2907], '2B-like': [2912], '(2B/2B-like)': [2913], 'extended': [2917], 'sub-structure': [2918], 'clamping': [2922, 2998], 'down': [2923, 2999], 'top': [2925], 'mostly': [2934], 'buried.': [2935], '2B/2B-like': [2942, 2994, 3040, 3174], 'mounted': [2945, 4256, 4306], 'hinges': [2952], 'pivot': [2958], 'configurations,': [2961], 'closed.': [2964], 'configuration,': [2968], 'accessible': [2978], 'binding,': [2980], 'thus,': [2982, 3246], 'load': [2986], 'sequence.': [2990, 3299], 'Once': [2991], 'bound,': [2992], 'close,': [2997], 'start.': [3006], 'These': [3007, 3384, 3763], 'open/closed': [3008], 'states': [3009, 3024, 3586], 'correspond': [3018], 'characterized': [3028], 'biochemically': [3029], 'based': [3030, 3613], 'protease': [3033], 'sensitivity': [3034], 'experiments': [3035], 'role:': [3045], 'surfaces': [3050], 'responsible': [3051, 3358, 3815, 3827], 'protein,': [3069, 3286, 3673, 3749], 'serves': [3070], 'Because': [3078], 'role,': [3081], 'often': [3084], 'protein".': [3089], 'regions,': [3091], 'TSA': [3093, 3168], 'TSB,': [3095], '(an': [3100], 'plasmid)': [3102, 4090], 'identified': [3104, 3131], 'translocation': [3108, 3140, 3162, 3703], 'signals,': [3109, 3163], 'presumably': [3111], 'system,': [3119, 3201, 3904, 4200, 4418], 'VirD4/TraD': [3125], '44.': [3127], 'Similar': [3128], '45.': [3137], 'Remarkably,': [3138, 4287], 'signals': [3141], 'located': [3144], 'C-': [3148], 'N-terminus': [3150], 'middle.': [3167], 'TSB': [3170], 'map': [3171, 3184], 'putative': [3178], 'VirD4-interacting': [3179], 'opposite': [3185], 'ssDNA-interacting': [3188], 'region,': [3189], 'available': [3195], 'even': [3202], '41,': [3207], '46.': [3208], 'Accessory': [3209], 'bigger': [3217], 'complex,': [3218, 4112], 'relaxosome,': [3220], '2–3': [3223], 'proteins,': [3225, 3275, 3545, 4132, 4390], '"accessory"': [3227], 'Much': [3233], 'refs.': [3242, 3514], '41),': [3244], 'short': [3249], 'description': [3250, 3901], 'given': [3253], 'case,': [3264], 'plasmid-encoded': [3274], 'TraY,': [3278], 'genome-encoded': [3280], '(integrated': [3282], 'host': [3283], 'factor)': [3284], 'heterodimeric': [3285], '~10-kDa': [3293], 'subunits,': [3294], '30%': [3296], 'identical': [3297], 'shows': [3309], 'induces': [3312], '160°': [3314], 'bend': [3315, 3599], '47.': [3319], 'small': [3323], '(131': [3325], 'residues),': [3326], 'structurally': [3327], '(RHH)': [3332], 'family,': [3333], 'bends': [3335], '50–55°': [3339], '48.': [3340], '127-residue': [3345], 'DNA,': [3353], 'tetramerization.': [3360], 'homodimerizes': [3365], 'RHH,': [3369], 'tetramers': [3373], 'cooperatively': [3377], 'minimal': [3380], '49.': [3383], 'multiple': [3391, 3531], '(summarized': [3395], '50': [3398], '(F': [3412], 'OriT),': [3413], 'IHF-binding': [3417], '(IHF': [3419], 'B),': [3422], 'TraY-binding': [3424], '(sbyA': [3426], 'sbyC),': [3428], 'TraM-binding': [3430], '(sbmA-C),': [3432], 'TraI-loading': [3438], 'following:': [3450], 'sbmA,': [3451], 'sbmB,': [3452], 'B,': [3454], 'sbmC,': [3455], 'sbyA,': [3456], 'sbyC,': [3457], 'A,': [3459], 'TraI-binding': [3460], '(TraI-A),': [3462], 'loading': [3467], '(TraI-B).': [3469], 'order': [3481], '51,': [3482], '52': [3483], 'distort': [3485], 'severely': [3488], 'locally,': [3490], 'leading': [3491], 'disruption': [3493], 'supercoiled': [3496], 'states.': [3499], 'Although': [3500, 4404], '24,': [3516], '37),': [3517], 'little': [3519], 'how': [3523], 'interact': [3526, 3736], 'other.': [3529], 'reports': [3532], 'activity': [3537], 'stimulated': [3539], 'direct': [3547], '33,': [3552], '52-54.': [3553], 'matter': [3556], 'fact,': [3558], 'everything': [3559], 'points': [3560], 'extremely': [3566], 'structure:': [3568], 'stable': [3575], 'oligomeric': [3576], 'states,': [3577], 'others': [3580, 4326], 'adopt': [3583], 'oligomerization': [3585], 'upon': [3587], 'DNA;': [3589], 'able': [3597, 3959, 3981, 4013], 'quite': [3601], 'severely,': [3602], 'implying': [3603], 'apparently': [3608], 'distant': [3609], 'linear': [3616], 'fact': [3627], 'Solving': [3631], 'greatest': [3642], 'challenges': [3643], 'How': [3647], 'recruited': [3651], 'apparatus': [3656], 'itself': [3657, 3674], 'investigated': [3660, 4540], '55).': [3665], 'integral': [3676, 3886], '56.': [3682], 'interacts': [3686], 'tail': [3690, 3784], 'VirD4/TraD,': [3692], 'visualized': [3697], 'crystallographically': [3698], '57.': [3699], 'signal': [3704], 'sequences,': [3705], 'whether': [3707], 'directly': [3712], 'above).': [3718], 'TrwA': [3722], '(a': [3723, 4208], 'homologue': [3725], 'TraM)': [3727], '(the': [3730, 4304, 4310], 'relaxase)': [3731], 'VirD4/TrwB': [3738], '58.': [3739], 'pneumophila,': [3753, 4630], 'chaperones': [3758], 'revealed': [3761], '59.': [3762], 'reminiscent': [3766], 'TraM/TraD': [3769], 'Lu': [3772], '57': [3775], 'VirD4.': [3786], 'Early': [3790], 'research,': [3793], 'became': [3795], 'apparent': [3796], 'divided': [3805], 'sets:': [3808], '(mpf)': [3813], 'biogenesis': [3818, 3979], 'junctions,': [3821], 'DNA-transfer': [3823], '(dtr)': [3825], 'processing': [3829], '60,': [3834], '61.': [3835], 'MPF': [3837], 'now': [3840], 'DTR': [3850], 'Linking': [3857], '(CP),': [3866], 'recruits': [3868], 'presents': [3872], 'seems': [3880, 4420], 'constitutive': [3888], '56,': [3894], 'treating': [3907], 'separately.': [3909], 'Also,': [3910], 'because': [3911], 'VirB1-11/VirD4': [3914, 4368], 'exhaustive': [3921], '62,': [3923], '63,': [3924], 'describing': [3929, 4344], 'subassemblies': [3932], 'assemblies': [3934], 'form,': [3941], 'unravelled': [3948], 'recently.': [3949], 'unique': [3953], 'DNAs.': [3965], 'Functionally,': [3966], 'cumulate': [3968, 4015], 'many': [3969, 4388], 'functions:': [3973], 'machines': [3980], 'construct': [3983], 'retract': [3985], 'thousands': [3989], 'subunits;': [3992], 'act': [3995, 4002], 'transporters;': [3998], '(iii)': [4000], 'transporters.': [4005], 'Conjugative': [4006], 'remarkable': [4010], 'apparatus.': [4021], 'No': [4022], 'wonder': [4023], 'architecture': [4027, 4300], 'immensely': [4029], 'than': [4032], 'any': [4033], 'review': [4042, 4051, 4071, 4506], 'Costa': [4044], '11': [4047], 'Galan': [4061], '64': [4064], 'machines,': [4067], 'focused': [4070], 'targeted': [4072], 'larger': [4075, 4195, 4217], 'audience).': [4076], 'subassembly': [4080, 4196], '(that': [4085], 'pKM101': [4089], '2009': [4094], 'Fronzes': [4096], '13': [4099], 'published': [4100, 4193], 'purification': [4102], 'subsequent': [4104], 'characterization': [4105], 'cryo-EM.': [4110], 'named': [4113], 'complex"': [4116], '1.05': [4118], 'megadaltons': [4119], 'size': [4121], 'copies': [4126, 4267], 'VirB': [4131, 4223], 'VirB9,': [4133], 'lipoprotein': [4137], 'VirB7.': [4138], 'Being': [4139], '(VirB10': [4148], 'indeed': [4150], 'seen': [4151, 4489], 'channel': [4154, 4574], '65': [4159], 'trans-membrane': [4163, 4279], 'helix': [4164], 'inserted': [4165, 4581], 'membrane),': [4169], 'thought': [4173], 'channel.': [4179], 'notion': [4181], 'prevailed': [4182], 'years': [4187], 'until': [4188], 'Low': [4189], 'closely': [4210], 'homologous': [4211], 'pKM101).': [4215], 'VirB3-10.': [4225], '(renamed': [4237], '(OM)': [4239], '(OMCC)': [4242, 4535], 'primarily': [4246], 'directed': [4247], 'towards': [4248], 'OM)': [4250], 'via': [4257, 4315], 'VirB5,': [4272], 'helices': [4280], 'emanating': [4283], 'OMCC.': [4286], 'VirB4': [4289, 4312], 'ATPase': [4290], 'hexameric': [4293, 4313], 'barrels': [4294], 'protruding': [4295], 'cytoplasm.': [4298], 'head': [4303], 'OMCC)': [4305], 'legs': [4309], 'barrels)': [4314], 'stalk/neck': [4317], 'unprecedented': [4319], 'concentric': [4331], 'stacks': [4332], 'rings': [4334], 'extending': [4335], 'cytosol': [4338], 'milieu.': [4342], 'systems,': [4346, 4517], 'convenient': [4349], '(albeit': [4350], 'imperfect)': [4351], 'categorize': [4353], 'them': [4354], 'classes:': [4357], 'B.': [4360], 'T4AS': [4361, 4375, 4417, 4438, 4445, 4473, 4485, 4514, 4538], 'broadly': [4363], 'consist': [4364], '(one': [4370], 'exception': [4371, 4625], 'more).': [4379], 'T4BS': [4380, 4425, 4429, 4495, 4516], 'generally': [4383], 'consisting': [4386], 'example': [4392], '27': [4393], 'Dot/Icm': [4396, 4455], 'L.': [4400, 4629], '8,': [4402], '66.': [4403], 'cag': [4406, 4464, 4611], 'H.': [4410, 4609], 'pylori': [4411, 4610], 'initially': [4413], 'classed': [4414], 'class.': [4427], 'Nevertheless,': [4428], 'VirB/VirD4': [4435], 'systems;': [4439], 'example,': [4441], 'archetypal': [4444], 'ATPases,': [4446], 'VirB11,': [4450], 'homologues': [4452], '(DotO,': [4457], 'DotL': [4458], 'DotB,': [4460], '(CagE,': [4466], 'Cag5': [4467], 'Cagα).': [4469], 'plasmid,': [4479], 'VirB11': [4480], 'absent.': [4482], 'overall,': [4484], '"minimal"': [4491], 'expanded': [4498], 'elaborated': [4501], 'versions.': [4502], 'Below,': [4503], 'OMCCs': [4510], 'IMCs': [4512], 'pointing': [4518], 'differences': [4521], 'classes': [4525], 'common': [4529], 'features.': [4530], 'so': [4541], 'far,': [4542], 'cage': [4547, 4570], 'layers,': [4550], 'O-': [4552], 'I-layers.': [4554], 'lining': [4565], 'interior': [4567], 'forming': [4572], '3A).': [4586], 'ring': [4589], 'buttressed': [4591], 'exterior': [4594], 'binary': [4596], 'VirB7': [4599], 'VirB9.': [4601], 'Most': [4602], '(pKM101,': [4605], 'R388,': [4606], 'tumefaciens,': [4608], 'Xanthomonas': [4613], 'citri)': [4614], '14-fold': [4616], 'symmetry': [4617, 4634], '13,': [4618], '16,': [4619], '65,': [4620], '67,': [4621], '68;': [4622], '13-fold': [4633], '15,': [4635], '69.': [4636], '3.': [4638], 'arch': [4640]}, 'cited_by_api_url': 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