Title: Fluorescence Resonance Energy Transfer (FRET) Spectroscopy
Abstract: Fluorescence or Förster resonance energy transfer (FRET) is an important spectroscopic technique with a history of practical application for more than half a century. In this article, the basic theory of FRET is elaborated for understanding this long-range nonradiative energy transfer phenomenon. The FRET rate and efficiency show strong distance dependence within the range 20–60 Å. Based on this critical property, FRET has opened new biological applications. Herein, three typical applications are described in detail, including molecular ruler, fluorescent protein sensing, and single-molecule detection.
Publication Year: 2018
Publication Date: 2018-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 2
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot