Title: Helicobacter pylori의 항생제 내성률 및 Clarithromycin 내성에 연관된 23S rRNA 돌연변이의 검출
Abstract: Background/Aims: It was recently reported that A to G transition mutations at position 2143 or 2144 of 23S rRNA gene are associated with clarithromycin resistance in Helicobacter pylori. The aims of this study were to determine the antibiotic resistance rates in H. pylori and to examine the causes of antibiotic resistance in clarithromycin resistant strains in Korea. Methods: Two hundred H. pylori strains were isolated from gastric biopsy specimens of one hundred patients with gastritis and peptic ulcer. The susceptibility of isolated H. pylori strains to clarithromycin and amoxicillin was evaluated by E test and disk diffusion method. To detect mutations in H. pylori 23S rRNA, polymerase chain reaction-restriction fragment length polymorphism was performed. For clinical isolates confirmed to have mutations or antibiotic resistance, mutations in individual colonies were analysed by direct sequencing. Results: The resistance rate of isolated strains for clarithromycin and amoxicillin was 4% and 0%, respectively. Three of four clarithromycin resistant strains of H. pylori isolated from different patients showed the A2144G mutation. One patient who had clarithromycin resistance showed C2216T mutation. In two patients, clarithromycin sensitive strains and clarithromycin resistant strains were mixed. Conclusions: Mutations in 23S rRNA are associated with clarithromycin resistance in H. pylori. Clarithromycin resistance was caused by A2144G mutation mainly and by C2216T mutation possibly. Some patients might be infected with multiple H. pylori strains.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot