Title: Characterization of heat shock protein 27 in extracellular vesicles: a potential anti‐inflammatory therapy
Abstract: The FASEB JournalVolume 33, Issue 2 p. 1617-1630 ResearchFree to Read Characterization of heat shock protein 27 in extracellular vesicles: a potential anti-inflammatory therapy Correction(s) for this article Erratum Volume 34Issue 10The FASEB Journal pages: 14055-14056 First Published online: September 29, 2020 Chunhua Shi, Chunhua Shi Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorAnnegret Ulke-Lemée, Annegret Ulke-Lemée Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorJingti Deng, Jingti Deng Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorZarah Batulan, Zarah Batulan Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorEdward R. O'Brien, Corresponding Author Edward R. O'Brien [email protected] Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaCorrespondence: Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Health Research and Innovation Center, Room GAA16, 3280 Hospital Dr. NW, Calgary, AB T2N 4Z6, Canada. E-mail: [email protected]Search for more papers by this author Chunhua Shi, Chunhua Shi Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorAnnegret Ulke-Lemée, Annegret Ulke-Lemée Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorJingti Deng, Jingti Deng Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorZarah Batulan, Zarah Batulan Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaSearch for more papers by this authorEdward R. O'Brien, Corresponding Author Edward R. O'Brien [email protected] Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaCorrespondence: Department of Cardiac Sciences, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Health Research and Innovation Center, Room GAA16, 3280 Hospital Dr. NW, Calgary, AB T2N 4Z6, Canada. E-mail: [email protected]Search for more papers by this author First published: 06 September 2018 https://doi.org/10.1096/fj.201800987RCitations: 4 This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT Previously, we reported that elevated serum levels of heat shock protein 27 (HSP27) are predictive of a lower risk of having a heart attack, stroke, or death from cardiovascular disease. Moreover, augmenting HSP27 (or the murine ortholog, HSP25) attenuated experimental atherogenesis, reduced inflammation, and lowered cholesterol levels. Recently, we noted that HSP27 activates NF-κB via TLR-4, resulting in attenuation of plaque inflammation; however, the precise anti-atherosclerosis mechanisms mediated by extracellular HSP27 are incompletely understood. Our purpose in this study was to investigate the existence of HSP27 in extracellular vesicles (EVs) and whether HSP27 elicited atheroprotective effects on target cells. Here, we provide evidence that HSP27 localizes to EVs derived from THP-1 cells using transmission electron microscopy (TEM) and immunogold labeling, Western blotting, ELISA, and fluorescence-activated cell sorting. TEM imaging indicated that HSP27 is found at the exosomal membrane. Multiple reactor monitor-mass spectrometric analysis of large vesicles, which included microparticles and exosomes, isolated from human plasma, also led to detection of HSP27 using the unique signature peptide, R.LFDQAFGLPR.L. Studies using THP-1 and human embryonic kidney cells show that HSP27-laden exosomes significantly stimulated NF-κB activation (P < 0.001) and release of IL-10 (P < 0.0001), suggesting that HSP27 may be important exosomal cargo with beneficial anti-inflammatory effects.—Shi, C., Ulke-Lemée, A., Deng, J., Batulan, Z., O'Brien, E. R. Characterization of heat shock protein 27 in extracellular vesicles: a potential antiinflammatory therapy. FASEB J. 33,1617–1630 (2019). www.fasebj.org Citing Literature Supporting Information Filename Description fsb2fj201800987r-sup-0001.docxapplication/docx, 19.8 KB Supplementary Material 1 fsb2fj201800987r-sup-0002.pdfPDF document, 529.2 KB Supplementary Material 2 fsb2fj201800987r-sup-0003.pdfPDF document, 59.8 KB Supplementary Material 3 fsb2fj201800987r-sup-0004.pdfPDF document, 698 KB Supplementary Material 4 fsb2fj201800987r-sup-0005.pdfPDF document, 101 KB Supplementary Material 5 fsb2fj201800987r-sup-0006.pdfPDF document, 3.4 MB Supplementary Material 6 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume33, Issue2February 2019Pages 1617-1630 RelatedInformation
Publication Year: 2018
Publication Date: 2018-09-06
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 57
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