Title: Abstract 15168: Interleukin-18 Enhances Mitochondrial Function in Pressure Overload through AMP-activated Protein Kinase Activation
Abstract: Introduction: We have reported that serum Interleukin-18 (IL-18) concentrations increased according to worsening heart failure (HF) functional class. However, the pathological roles of serum IL-18 elevation in HF have not been fully elucidated. The purpose of this study was to elucidate the role of IL-18 in adaptation to pressure overload in IL-18 null mice. Hypothesis: We hypothesized that IL-18 plays a key role in pressure overload-induced hypertrophic responses by enhancing mitochondrial function. Methods: Ten-week-old male C57BL6/J-background wild type (WT) mice and C57BL6/J-background IL-18 null (IL-18-/-) were subjected to transaortic constriction (TAC). Cardiac function was assessed by transthoracic echocardiography every week. Two weeks after TAC, myocardial samples were obtained. Detection of cardiac gene expressions by q-RT PCR and quantification of phospho-proteins by western blot were performed. An AMPK activator (AICAR 100mg/kg,) was given intraperitoneally everyday after TAC procedure. Resul...
Publication Year: 2014
Publication Date: 2014-11-25
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot