Thermal proteome profiling in bacteria: probing protein state <i>in vivo</i>

Name: Work Video:
Duration: 3 min 30 s
André Mateus; Jacob Bobonis; Nils Kurzawa; Frank Stein; Dominic Helm; Johannes F. Hevler; Athanasios Typas; Mikhail M. Savitski
{'id': 'https://openalex.org/W2873517389', 'doi': 'https://doi.org/10.15252/msb.20188242', 'title': 'Thermal proteome profiling in bacteria: probing protein state <i>in vivo</i>', 'display_name': 'Thermal proteome profiling in bacteria: probing protein state <i>in vivo</i>', 'publication_year': 2018, 'publication_date': '2018-07-01', 'ids': {'openalex': 'https://openalex.org/W2873517389', 'doi': 'https://doi.org/10.15252/msb.20188242', 'mag': '2873517389', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/29980614', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/6056769'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/msb.20188242', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S200893805', 'display_name': 'Molecular Systems Biology', 'issn_l': '1744-4292', 'issn': ['1744-4292'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://doi.org/10.15252/msb.20188242', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5051138564', 'display_name': 'André Mateus', 'orcid': 'https://orcid.org/0000-0001-6870-0677'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'André Mateus', 'raw_affiliation_strings': ['Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5064896760', 'display_name': 'Jacob Bobonis', 'orcid': 'https://orcid.org/0000-0001-9293-9733'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}, {'id': 'https://openalex.org/I223822909', 'display_name': 'Heidelberg University', 'ror': 'https://ror.org/038t36y30', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I223822909']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Jacob Bobonis', 'raw_affiliation_strings': ['Faculty of Biosciences Heidelberg University Heidelberg Germany', 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}, {'raw_affiliation_string': 'Faculty of Biosciences Heidelberg University Heidelberg Germany', 'institution_ids': ['https://openalex.org/I223822909']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5089774776', 'display_name': 'Nils Kurzawa', 'orcid': 'https://orcid.org/0000-0002-7846-2817'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}, {'id': 'https://openalex.org/I223822909', 'display_name': 'Heidelberg University', 'ror': 'https://ror.org/038t36y30', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I223822909']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Nils Kurzawa', 'raw_affiliation_strings': ['Faculty of Biosciences Heidelberg University Heidelberg Germany', 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}, {'raw_affiliation_string': 'Faculty of Biosciences Heidelberg University Heidelberg Germany', 'institution_ids': ['https://openalex.org/I223822909']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5066853663', 'display_name': 'Frank Stein', 'orcid': 'https://orcid.org/0000-0001-9695-1692'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Frank Stein', 'raw_affiliation_strings': ['Proteomics Core Facility European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Proteomics Core Facility European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5031234897', 'display_name': 'Dominic Helm', 'orcid': 'https://orcid.org/0000-0001-9321-2069'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Dominic Helm', 'raw_affiliation_strings': ['Proteomics Core Facility European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Proteomics Core Facility European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5001113054', 'display_name': 'Johannes F. Hevler', 'orcid': 'https://orcid.org/0000-0001-6636-7145'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Johannes Hevler', 'raw_affiliation_strings': ['Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5046083835', 'display_name': 'Athanasios Typas', 'orcid': 'https://orcid.org/0000-0002-0797-9018'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Athanasios Typas', 'raw_affiliation_strings': ['Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5007602594', 'display_name': 'Mikhail M. Savitski', 'orcid': 'https://orcid.org/0000-0003-2011-9247'}, 'institutions': [{'id': 'https://openalex.org/I4210138560', 'display_name': 'European Molecular Biology Laboratory', 'ror': 'https://ror.org/03mstc592', 'country_code': 'DE', 'type': 'government', 'lineage': ['https://openalex.org/I4210138560']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Mikhail M Savitski', 'raw_affiliation_strings': ['Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany'], 'affiliations': [{'raw_affiliation_string': 'Genome Biology Unit European Molecular Biology Laboratory Heidelberg Germany', 'institution_ids': ['https://openalex.org/I4210138560']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 5000, 'currency': 'USD', 'value_usd': 5000, 'provenance': 'doaj'}, 'apc_paid': {'value': 5000, 'currency': 'USD', 'value_usd': 5000, 'provenance': 'doaj'}, 'fwci': 11.839, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 156, 'citation_normalized_percentile': {'value': 0.999951, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '14', 'issue': '7', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10521', 'display_name': 'RNA and protein synthesis mechanisms', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10521', 'display_name': 'RNA and protein synthesis mechanisms', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10015', 'display_name': 'Genomics and Phylogenetic Studies', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10120', 'display_name': 'Bacterial Genetics and Biotechnology', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/proteome', 'display_name': 'Proteome', 'score': 0.72817993}, {'id': 'https://openalex.org/keywords/profiling', 'display_name': 'Profiling (computer programming)', 'score': 0.43689746}], 'concepts': [{'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.8569601}, {'id': 'https://openalex.org/C104397665', 'wikidata': 'https://www.wikidata.org/wiki/Q860947', 'display_name': 'Proteome', 'level': 2, 'score': 0.72817993}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.5690911}, {'id': 'https://openalex.org/C207001950', 'wikidata': 'https://www.wikidata.org/wiki/Q141124', 'display_name': 'In vivo', 'level': 2, 'score': 0.50503856}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.46363634}, {'id': 'https://openalex.org/C2993175405', 'wikidata': 'https://www.wikidata.org/wiki/Q8054', 'display_name': 'Bacterial protein', 'level': 3, 'score': 0.45460197}, {'id': 'https://openalex.org/C187191949', 'wikidata': 'https://www.wikidata.org/wiki/Q1138496', 'display_name': 'Profiling (computer programming)', 'level': 2, 'score': 0.43689746}, {'id': 'https://openalex.org/C46111723', 'wikidata': 'https://www.wikidata.org/wiki/Q471857', 'display_name': 'Proteomics', 'level': 3, 'score': 0.43673253}, {'id': 'https://openalex.org/C60644358', 'wikidata': 'https://www.wikidata.org/wiki/Q128570', 'display_name': 'Bioinformatics', 'level': 1, 'score': 0.26533005}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.20129877}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.19405574}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.13179156}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C111919701', 'wikidata': 'https://www.wikidata.org/wiki/Q9135', 'display_name': 'Operating system', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000254', 'qualifier_name': 'growth & development', 'is_major_topic': True}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D040901', 'descriptor_name': 'Proteomics', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D003593', 'descriptor_name': 'Cytoplasm', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003593', 'descriptor_name': 'Cytoplasm', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015964', 'descriptor_name': 'Gene Expression Regulation, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018507', 'descriptor_name': 'Gene Expression Regulation, Developmental', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D055550', 'descriptor_name': 'Protein Stability', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D040901', 'descriptor_name': 'Proteomics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013816', 'descriptor_name': 'Thermodynamics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D044366', 'descriptor_name': 'Transition Temperature', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/msb.20188242', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S200893805', 'display_name': 'Molecular Systems Biology', 'issn_l': '1744-4292', 'issn': ['1744-4292'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc6056769', 'pdf_url': 'https://europepmc.org/articles/pmc6056769?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056769', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/29980614', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/msb.20188242', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S200893805', 'display_name': 'Molecular Systems Biology', 'issn_l': '1744-4292', 'issn': ['1744-4292'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [{'funder': 'https://openalex.org/F4320314023', 'funder_display_name': 'European Molecular Biology Laboratory', 'award_id': None}], 'datasets': [], 'versions': [], 'referenced_works_count': 123, 'referenced_works': ['https://openalex.org/W1243735121', 'https://openalex.org/W1539476045', 'https://openalex.org/W1546783991', 'https://openalex.org/W1554652307', 'https://openalex.org/W1557963254', 'https://openalex.org/W1639048060', 'https://openalex.org/W1661654806', 'https://openalex.org/W1829650984', 'https://openalex.org/W1855620190', 'https://openalex.org/W1864838098', 'https://openalex.org/W1966124059', 'https://openalex.org/W1966863706', 'https://openalex.org/W1968759341', 'https://openalex.org/W1973079886', 'https://openalex.org/W1976744814', 'https://openalex.org/W1980510165', 'https://openalex.org/W1982029231', 'https://openalex.org/W1984839716', 'https://openalex.org/W1986340051', 'https://openalex.org/W1988146323', 'https://openalex.org/W1993251792', 'https://openalex.org/W1998018574', 'https://openalex.org/W2000802512', 'https://openalex.org/W2001191925', 'https://openalex.org/W2006805047', 'https://openalex.org/W2013848537', 'https://openalex.org/W2016800541', 'https://openalex.org/W2018131055', 'https://openalex.org/W2023849113', 'https://openalex.org/W2026771908', 'https://openalex.org/W2028075953', 'https://openalex.org/W2030015794', 'https://openalex.org/W2038041834', 'https://openalex.org/W2038613897', 'https://openalex.org/W2039045853', 'https://openalex.org/W2040964348', 'https://openalex.org/W2043981786', 'https://openalex.org/W2048593866', 'https://openalex.org/W2048614879', 'https://openalex.org/W2063624353', 'https://openalex.org/W2072252061', 'https://openalex.org/W2079228339', 'https://openalex.org/W2080737411', 'https://openalex.org/W2081851349', 'https://openalex.org/W2085555089', 'https://openalex.org/W2092435069', 'https://openalex.org/W2093064281', 'https://openalex.org/W2093623840', 'https://openalex.org/W2098146447', 'https://openalex.org/W2099303284', 'https://openalex.org/W2109809698', 'https://openalex.org/W2110151487', 'https://openalex.org/W2111967267', 'https://openalex.org/W2116201639', 'https://openalex.org/W2117087393', 'https://openalex.org/W2120069280', 'https://openalex.org/W2122598723', 'https://openalex.org/W2124348590', 'https://openalex.org/W2128371743', 'https://openalex.org/W2130725058', 'https://openalex.org/W2131321938', 'https://openalex.org/W2134511156', 'https://openalex.org/W2136861926', 'https://openalex.org/W2138214676', 'https://openalex.org/W2142261128', 'https://openalex.org/W2142836135', 'https://openalex.org/W2146512944', 'https://openalex.org/W2146923856', 'https://openalex.org/W2148868102', 'https://openalex.org/W2149191396', 'https://openalex.org/W2159675211', 'https://openalex.org/W2160656989', 'https://openalex.org/W2164990150', 'https://openalex.org/W2165672560', 'https://openalex.org/W2166387637', 'https://openalex.org/W2166846071', 'https://openalex.org/W2173488267', 'https://openalex.org/W2191824721', 'https://openalex.org/W2195872026', 'https://openalex.org/W2216761081', 'https://openalex.org/W2235470599', 'https://openalex.org/W2275287916', 'https://openalex.org/W2282925852', 'https://openalex.org/W2293847040', 'https://openalex.org/W2300011769', 'https://openalex.org/W2312280072', 'https://openalex.org/W2315534050', 'https://openalex.org/W2335262420', 'https://openalex.org/W2335542885', 'https://openalex.org/W2344645960', 'https://openalex.org/W2440453788', 'https://openalex.org/W2519691041', 'https://openalex.org/W2522417827', 'https://openalex.org/W2523811837', 'https://openalex.org/W2524972396', 'https://openalex.org/W2525566551', 'https://openalex.org/W2537623931', 'https://openalex.org/W2542787251', 'https://openalex.org/W2548029401', 'https://openalex.org/W2560707317', 'https://openalex.org/W2577168473', 'https://openalex.org/W2582743722', 'https://openalex.org/W2588469583', 'https://openalex.org/W2589042007', 'https://openalex.org/W2590059905', 'https://openalex.org/W2593536290', 'https://openalex.org/W2607462092', 'https://openalex.org/W2613666205', 'https://openalex.org/W2717775281', 'https://openalex.org/W2765883456', 'https://openalex.org/W2768421721', 'https://openalex.org/W2768799378', 'https://openalex.org/W2776569579', 'https://openalex.org/W2779019546', 'https://openalex.org/W2782205417', 'https://openalex.org/W2787587553', 'https://openalex.org/W2791501381', 'https://openalex.org/W2792021903', 'https://openalex.org/W2793385742', 'https://openalex.org/W2800980602', 'https://openalex.org/W2804515387', 'https://openalex.org/W3104725839', 'https://openalex.org/W4240480198'], 'related_works': ['https://openalex.org/W4390205769', 'https://openalex.org/W3022402204', 'https://openalex.org/W2391458050', 'https://openalex.org/W2347437365', 'https://openalex.org/W2124137418', 'https://openalex.org/W2114732768', 'https://openalex.org/W2106414650', 'https://openalex.org/W2086860678', 'https://openalex.org/W1999412725', 'https://openalex.org/W1982829397'], 'abstract_inverted_index': {'Article24': [0], 'July': [1], '2018Open': [2], 'Access': [3], 'Transparent': [4], 'process': [5, 479], 'Thermal': [6, 673, 1452, 1458], 'proteome': [7, 520, 539, 590, 674, 711, 1009, 1079, 1453, 1459, 1489, 1598, 1905, 1949, 1956, 2760, 3139, 4093], 'profiling': [8, 521, 675, 1010, 1454, 1460], 'in': [9, 14, 531, 566, 580, 597, 630, 633, 658, 687, 732, 747, 796, 817, 955, 1001, 1054, 1107, 1164, 1203, 1383, 1435, 1455, 1615, 1678, 1684, 1765, 1778, 1857, 1863, 1923, 2125, 2131, 2175, 2202, 2218, 2337, 2439, 2449, 2503, 2563, 2601, 2608, 2721, 2735, 2817, 2823, 2857, 2860, 2867, 2885, 2930, 2956, 2971, 3025, 3057, 3086, 3098, 3107, 3145, 3155, 3164, 3189, 3201, 3206, 3209, 3220, 3225, 3231, 3326, 3330, 3345, 3356, 3368, 3401, 3431, 3441, 3462, 3515, 3560, 3586, 3591, 3691, 3695, 3827, 3833, 3885, 3891, 3903, 4044, 4098, 4123, 4160, 4194, 4229, 4259, 4265, 4266, 4304, 4350], 'bacteria:': [10], 'probing': [11, 2483], 'protein': [12, 544, 563, 571, 662, 692, 695, 720, 724, 729, 738, 1165, 1313, 1508, 1655, 1730, 1805, 1823, 2020, 2484, 2736, 2741, 2796, 2818, 3276, 3281, 3453, 3562, 3620, 3629, 3710, 3720, 3745, 3751, 3801, 3824, 3863, 3991, 4057, 4299, 4318, 4360], 'state': [13], 'vivo': [15, 634, 4267], 'André': [16, 18, 204, 206, 394], 'Mateus': [17, 19, 205, 207], 'orcid.org/0000-0001-6870-0677': [20, 208], 'Genome': [21, 41, 69, 136, 161, 188, 209, 229, 257, 324, 349, 376], 'Biology': [22, 26, 42, 46, 70, 74, 101, 121, 137, 141, 162, 166, 189, 193, 210, 214, 230, 234, 258, 262, 289, 309, 325, 329, 350, 354, 377, 381, 415, 419, 435, 461], 'Unit,': [23, 43, 71, 138, 163, 190, 211, 231, 259, 326, 351, 378, 416], 'European': [24, 44, 72, 99, 119, 139, 164, 191, 212, 232, 260, 287, 307, 327, 352, 379, 417, 433], 'Molecular': [25, 45, 73, 100, 120, 140, 165, 192, 213, 233, 261, 288, 308, 328, 353, 380, 418, 434, 459], 'Laboratory,': [27, 47, 75, 102, 122, 142, 167, 194, 215, 235, 263, 290, 310, 330, 355, 382, 420, 436], 'Heidelberg,': [28, 48, 55, 76, 83, 103, 123, 143, 168, 195, 216, 236, 243, 264, 271, 291, 311, 331, 356, 383, 421, 428, 437], 'Germany': [29, 49, 56, 77, 84, 104, 124, 144, 169, 196, 217, 237, 244, 265, 272, 292, 312, 332, 357, 384, 422, 429, 438], 'Search': [30, 57, 85, 105, 125, 145, 170, 197, 218, 245, 273, 293, 313, 333, 358, 385], 'for': [31, 58, 86, 106, 126, 146, 171, 198, 219, 246, 274, 294, 314, 334, 359, 386, 506, 509, 812, 887, 902, 905, 982, 997, 1156, 1241, 1252, 1371, 1517, 1522, 1530, 1653, 1728, 1876, 1886, 1937, 2007, 2046, 2054, 2281, 3541, 3698, 3714, 3897, 3972, 4224], 'more': [32, 59, 87, 107, 127, 147, 172, 199, 220, 247, 275, 295, 315, 335, 360, 387, 537, 713, 1058, 1951, 2359, 2727, 2762, 3040, 3732, 4012, 4132, 4164, 4212], 'papers': [33, 60, 88, 108, 128, 148, 173, 200, 221, 248, 276, 296, 316, 336, 361, 388], 'by': [34, 61, 89, 109, 129, 149, 174, 201, 222, 249, 277, 297, 317, 337, 362, 389, 802, 1350, 1618, 1626, 1687, 1701, 1833, 2571, 2641, 2838, 2961, 3174, 3214, 3251, 3335, 3450, 3760, 3998, 4069], 'this': [35, 62, 90, 110, 130, 150, 175, 202, 223, 250, 278, 298, 318, 338, 363, 390, 1756, 1971, 2469, 3003, 3043, 3402, 3459, 3577, 3610, 4311], 'author': [36, 63, 91, 111, 131, 151, 176, 203, 224, 251, 279, 299, 319, 339, 364, 391, 487], 'Jacob': [37, 39, 225, 227, 396], 'Bobonis': [38, 40, 226, 228], 'Faculty': [50, 78, 238, 266], 'of': [51, 79, 239, 267, 424, 465, 475, 515, 527, 562, 604, 617, 639, 685, 728, 752, 759, 765, 835, 854, 874, 885, 889, 892, 895, 973, 1020, 1071, 1077, 1093, 1095, 1104, 1114, 1117, 1126, 1161, 1245, 1256, 1273, 1282, 1326, 1418, 1430, 1479, 1487, 1507, 1537, 1541, 1550, 1554, 1579, 1590, 1594, 1612, 1649, 1681, 1691, 1724, 1762, 1775, 1781, 1788, 1802, 1818, 1854, 1889, 1897, 1901, 1913, 1939, 1970, 2145, 2149, 2166, 2190, 2195, 2245, 2251, 2321, 2436, 2464, 2501, 2574, 2594, 2629, 2658, 2748, 2752, 2769, 2792, 2804, 2810, 2892, 2911, 2914, 2940, 2965, 3019, 3112, 3130, 3143, 3240, 3254, 3298, 3310, 3348, 3446, 3477, 3491, 3509, 3554, 3581, 3619, 3628, 3635, 3644, 3646, 3685, 3687, 3709, 3744, 3750, 3769, 3785, 3800, 3810, 3819, 3823, 3847, 3862, 3872, 3882, 3899, 3948, 3958, 3980, 3996, 4006, 4011, 4021, 4042, 4049, 4063, 4072, 4088, 4112, 4203, 4291, 4295, 4317, 4325, 4328], 'Biosciences,': [52, 80, 240, 268, 425], 'Heidelberg': [53, 81, 241, 269, 426], 'University,': [54, 82, 242, 270, 427], 'Nils': [64, 66, 252, 254, 398], 'Kurzawa': [65, 67, 253, 255], 'orcid.org/0000-0002-7846-2817': [68, 256], 'Frank': [92, 94, 280, 282, 400], 'Stein': [93, 95, 281, 283], 'Proteomics': [96, 116, 284, 304], 'Core': [97, 117, 285, 305, 431], 'Facility,': [98, 118, 286, 306, 432], 'Dominic': [112, 114, 300, 302, 402], 'Helm': [113, 115, 301, 303], 'Johannes': [132, 134, 320, 322, 404], 'Hevler': [133, 135, 321, 323], 'Athanasios': [152, 156, 340, 344, 406], 'Typas': [153, 157, 341, 345, 407], 'Corresponding': [154, 180, 342, 368], 'Author': [155, 181, 343, 369, 392], '[email': [158, 185, 346, 373, 447, 457], 'protected]': [159, 186, 347, 374, 448, 458], 'orcid.org/0000-0002-0797-9018': [160, 348], 'Mikhail': [177, 182, 365, 370, 410], 'M': [178, 183, 366, 371, 411], 'Savitski': [179, 184, 367, 372, 412, 1012], 'orcid.org/0000-0003-2011-9247': [187, 375], 'Information': [393], 'Mateus1,': [395], 'Bobonis1,2,': [397], 'Kurzawa1,2,': [399], 'Stein3,': [401], 'Helm3,': [403], 'Hevler1,': [405], '*,1': [408, 413], 'and': [409, 468, 486, 512, 589, 615, 623, 632, 643, 667, 704, 770, 793, 798, 815, 868, 958, 986, 1053, 1073, 1119, 1175, 1220, 1251, 1259, 1270, 1289, 1295, 1312, 1355, 1369, 1428, 1482, 1512, 1545, 1558, 1577, 1586, 1770, 1842, 2030, 2050, 2130, 2226, 2287, 2291, 2293, 2318, 2382, 2424, 2523, 2636, 2663, 2776, 2798, 2866, 2897, 2909, 2920, 2922, 2925, 2992, 3050, 3052, 3071, 3095, 3138, 3147, 3166, 3185, 3233, 3249, 3351, 3374, 3391, 3486, 3488, 3504, 3512, 3519, 3545, 3564, 3572, 3694, 3702, 3717, 3755, 3783, 3792, 3845, 3854, 3927, 3969, 3984, 4002, 4040, 4095, 4158, 4170, 4189, 4210, 4218, 4237, 4262, 4362], '1Genome': [414], '2Faculty': [423], '3Proteomics': [430], '*Corresponding': [439, 449], 'author.': [440, 450], 'Tel:': [441, 451], '+49': [442, 452], '6221': [443, 453], '387': [444, 454], '8156;': [445], 'E-mail:': [446, 456], '8560;': [455], 'Systems': [460], '(2018)14:e8242https://doi.org/10.15252/msb.20188242': [462], 'PDFDownload': [463], 'PDF': [464], 'article': [466], 'text': [467], 'main': [469], 'figures.': [470], 'Peer': [471], 'ReviewDownload': [472], 'a': [473, 536, 550, 581, 602, 748, 766, 785, 865, 953, 994, 1004, 1069, 1153, 1158, 1213, 1268, 1419, 1442, 1468, 1477, 2176, 2193, 2272, 2413, 2418, 2461, 2480, 2517, 2540, 2591, 2609, 2749, 3015, 3082, 3382, 3661, 3731, 3808, 3828, 3870, 3886, 3946], 'summary': [474], 'the': [476, 525, 554, 558, 585, 683, 716, 756, 763, 776, 789, 806, 818, 833, 875, 883, 890, 898, 967, 971, 1018, 1021, 1055, 1074, 1078, 1090, 1101, 1217, 1221, 1243, 1276, 1323, 1327, 1335, 1352, 1356, 1416, 1426, 1483, 1488, 1505, 1542, 1546, 1555, 1559, 1570, 1574, 1580, 1584, 1595, 1638, 1645, 1650, 1713, 1720, 1725, 1766, 1834, 1839, 1902, 1940, 1946, 1954, 1968, 2014, 2083, 2146, 2150, 2161, 2170, 2187, 2191, 2203, 2219, 2240, 2243, 2246, 2305, 2351, 2437, 2440, 2465, 2499, 2510, 2544, 2557, 2572, 2625, 2630, 2650, 2659, 2669, 2685, 2702, 2708, 2711, 2746, 2753, 2757, 2765, 2802, 2808, 2811, 2843, 2868, 2912, 2915, 2931, 2937, 2962, 2969, 2982, 2993, 3026, 3046, 3058, 3065, 3087, 3113, 3116, 3134, 3175, 3180, 3226, 3238, 3246, 3284, 3296, 3299, 3302, 3307, 3357, 3369, 3406, 3420, 3444, 3475, 3489, 3548, 3565, 3584, 3625, 3636, 3642, 3647, 3651, 3678, 3688, 3727, 3761, 3766, 3776, 3780, 3786, 3790, 3798, 3820, 3838, 3842, 3848, 3852, 3860, 3904, 3937, 3940, 3959, 3994, 4018, 4037, 4045, 4061, 4070, 4099, 4110, 4120, 4125, 4135, 4162, 4191, 4201, 4204, 4219, 4222, 4292, 4323], 'editorial': [477, 481], 'decision': [478, 482], 'including': [480, 612, 2117], 'letters,': [483], 'reviewer': [484], 'comments': [485], 'responses': [488], 'to': [489, 492, 523, 557, 636, 644, 660, 678, 681, 755, 842, 917, 1068, 1124, 1147, 1235, 1292, 1302, 1359, 1467, 1476, 1503, 1563, 1600, 1630, 1705, 1754, 1838, 1907, 1973, 1979, 1983, 2072, 2075, 2093, 2160, 2208, 2242, 2376, 2385, 2430, 2460, 2479, 2513, 2598, 2624, 2680, 2726, 2820, 2870, 2933, 2989, 3028, 3031, 3060, 3080, 3119, 3149, 3168, 3179, 3243, 3275, 3316, 3359, 3371, 3385, 3455, 3550, 3605, 3656, 4109, 4199, 4309], 'feedback.': [490], 'ToolsAdd': [491], 'favoritesDownload': [493], 'CitationsTrack': [494], 'CitationsPermissions': [495], 'ShareFacebookTwitterLinked': [496], 'InMendeleyWechatReddit': [497], 'Figures': [498], '&': [499, 846, 935, 1404, 2060, 2396, 2400, 3102, 3124, 3292, 3614], 'Info': [500], 'Abstract': [501], 'Increasing': [502], 'antibiotic': [503], 'resistance': [504, 962, 1308], 'urges': [505], 'new': [507, 810, 855, 862, 1246], 'technologies': [508], 'studying': [510, 970], 'microbes': [511], 'antimicrobial': [513, 641, 1249], 'mechanism': [514], 'action.': [516], 'We': [517, 990, 1230, 1373, 1413, 1931, 2800, 2878, 3320, 3631], 'adapted': [518, 677, 1334], 'thermal': [519, 757, 1008, 1023, 1102, 2485, 3665], '(TPP)': [522], 'probe': [524, 661, 682], 'thermostability': [526, 545, 614, 684, 721, 1116, 2237, 2568, 2768, 2797, 2809, 2824, 2886, 3527, 3539, 3587, 3626, 4062, 4094], 'Escherichia': [528, 679, 1293, 1318, 1456, 1767, 3135, 3756], 'coli': [529, 534, 587, 680, 710, 1319, 1329, 1402, 1457, 1524, 1544, 1557, 1597, 1768, 1783, 1878, 1904, 1948, 2168, 2197, 2506, 2759, 3136, 3693, 3719, 3757, 4298], 'proteins': [530, 686, 1376, 1659, 1734, 1763, 1777, 1790, 1892, 1914, 1942, 2009, 2053, 2108, 2123, 2198, 2229, 2299, 2356, 2438, 2502, 2770, 2777, 2834, 2881, 3144, 3311, 3323, 3353, 3365, 3439, 3467, 3513, 3634, 3686, 3770, 4329, 4342], 'vivo.': [532], 'E.': [533, 586, 709, 1328, 1401, 1523, 1543, 1556, 1596, 1782, 1798, 1816, 1877, 1903, 1947, 2167, 2196, 2505, 2758, 3692, 3718, 4297], 'had': [535, 576, 3067, 3072, 3522, 3599, 3730], 'thermostable': [538, 714, 1677, 1952, 2360, 2562, 2763, 3041, 4133, 4165, 4213], 'than': [540, 715, 1953, 2361, 2701, 2764, 4134, 4214], 'human': [541, 717, 790, 1547, 1560, 1955, 2008, 2182, 2766, 3696, 3716, 3754], 'cells,': [542], 'with': [543, 719, 784, 1031, 1248, 1362, 1395, 1441, 1622, 1660, 1697, 1735, 1791, 1810, 1813, 1821, 1860, 1915, 1967, 2019, 2042, 2271, 2405, 2417, 2543, 2590, 2684, 2772, 2778, 2882, 2952, 3414, 3558, 3589, 3807, 3869, 3945, 4036, 4102, 4252, 4344], 'depending': [546, 722], 'on': [547, 723, 788, 799, 912, 961, 1003, 2488, 2697, 2795, 2807, 3133, 4092, 4175], 'subcellular': [548, 725, 2774], 'location—forming': [549], 'high-to-low': [551], 'gradient': [552], 'from': [553, 1400, 1640, 1715, 1797, 1865, 2239, 2649, 2968, 3115, 3753, 3771, 4330, 4333], 'cell': [555, 918, 2132, 2248, 2525, 3066, 3088, 3566], 'surface': [556], 'cytoplasm.': [559], 'While': [560, 805], 'subunits': [561, 578, 745, 2320, 4020], 'complexes': [564, 572, 730, 739, 2942, 3511, 3546, 3621, 3711, 3721, 3729, 3746, 3752, 3802, 3825, 3864, 3883, 3901, 3962, 3976, 4009, 4058, 4156, 4300], 'residing': [565, 731], 'one': [567, 733, 873, 3202, 3645], 'compartment': [568, 734, 1533, 3831, 3889, 4101], 'melted': [569, 2110, 2174, 3712, 4024], 'similarly,': [570, 736], 'spanning': [573, 740], 'compartments': [574, 741, 3966], 'often': [575, 742, 869], 'their': [577, 646, 744, 956, 1260, 1601, 1771, 1908, 2773, 3526, 3533, 3561, 4064, 4080, 4176], 'melting': [579, 746, 1091, 1110, 1324, 1513, 1528, 1538, 1551, 1591, 1613, 1623, 1647, 1682, 1698, 1722, 1819, 1855, 1861, 1884, 1898, 1934, 2164, 2415, 2420, 3160, 3643, 3659, 3683, 3734, 3767, 3821, 4326], 'location-wise': [582, 749], 'manner.': [583, 750], 'Monitoring': [584], 'meltome': [588, 1320, 1769, 1784, 3137], 'at': [591, 878, 1083, 1198, 1378, 1384, 1509, 1661, 1736, 1976, 2056, 2111, 2421, 2451, 2468, 3002, 3042, 3215, 3221, 3283, 3301, 3331, 3336, 3458, 3576, 3609, 4345, 4351], 'different': [592, 1199, 1204, 3159, 3193, 3964], 'growth': [593, 2055, 2793, 2805, 3004, 3131, 3151, 3170, 3286, 3403, 3460, 3575, 3611], 'phases': [594], 'captured': [595], 'changes': [596, 1163, 3540, 3559], 'metabolism.': [598], 'Cells': [599], 'lacking': [600], 'TolC,': [601], 'component': [603, 1076], 'multiple': [605, 2106, 2143, 2274, 2410, 3438, 3834, 3892], 'efflux': [606, 3975, 4022], 'pumps,': [607], 'exhibited': [608], 'major': [609, 767, 876], 'physiological': [610, 1162], 'changes,': [611], 'differential': [613, 3387, 4038], 'levels': [616, 2964], 'its': [618, 760, 794, 2095, 2181, 3426, 4253], 'interaction': [619, 761], 'partners,': [620, 762], 'signaling': [621], 'cascades,': [622], 'periplasmic': [624, 772, 2298, 2348], 'quality': [625], 'control.': [626], 'Finally,': [627], 'we': [628, 651, 1087, 1287, 1333, 2407, 2496, 2537, 2585, 2814, 2853, 3278, 3537, 3623, 3676], 'combined': [629], 'vitro': [631], 'TPP': [635, 654, 1016, 1151, 1197, 1234, 1291, 1336, 1619, 1688, 1866, 2344, 2694, 2739], 'identify': [637], 'targets': [638, 1128], 'known': [640, 945, 2092, 2375, 2678, 3604, 4198], 'drugs': [642, 863], 'map': [645], 'downstream': [647, 706, 1261], 'effects.': [648, 707, 1262], 'In': [649, 775, 1051, 1145, 2104, 2158, 2350, 2755, 3273, 3362, 3535], 'conclusion,': [650], 'demonstrate': [652], 'that': [653, 1232, 1415, 2343, 2409, 2599, 2723, 2743, 3064, 3210, 3398, 3425, 3633, 3803, 3865, 3939, 3977, 3990, 4003, 4028], 'can': [655, 1088, 1122, 1211, 1299, 3007, 3649, 4172], 'be': [656, 1300, 1360, 2073, 2377, 2458, 2682, 2821, 2836, 3009, 3456, 4107], 'used': [657, 904, 1301, 1502, 3010], 'bacteria': [659, 978, 1236, 1364], 'complex': [663, 693, 1314, 2917, 3638, 3653, 3690, 3941, 4000, 4076, 4205], 'architecture,': [664, 694], 'metabolic': [665, 698, 1310, 3442, 3543], 'pathways,': [666], 'intracellular': [668, 700], 'drug': [669, 701, 705, 1127], 'target': [670, 702, 914], 'engagement.': [671], 'Synopsis': [672], 'is': [676, 712, 857, 864, 908, 943, 964, 979, 1080, 1490, 1500, 1534, 1893, 1965, 2091, 2569, 2761, 2950, 3022, 3109, 3428, 3602, 4034, 4197, 4250, 4302], 'vivo,': [688], 'yielding': [689], 'insights': [690, 1304], 'into': [691, 1216, 1305], 'activity,': [696, 1311, 3563], 'cellular': [697, 1022, 1218, 1532, 1602, 1909, 1925, 3830, 3888, 3906, 3965], 'state,': [699], 'engagement': [703, 915], 'The': [708, 1317, 1526, 1882, 2295, 2693, 3105, 3593], 'one,': [718, 2767], 'location.': [726], 'Subunits': [727], 'melt': [735, 2201, 3317, 3804, 3866], 'while': [737, 2738, 3341], 'have': [743, 991, 2788, 3312, 3606, 3657, 4060], 'Knockout': [751], 'tolC': [753, 4091], 'led': [754], 'destabilization': [758, 4071], 'downregulation': [764], 'porin': [768, 3932, 4227], '(OmpF),': [769], 'increased': [771, 2238, 2627, 2642], 'stress.': [773], 'Introduction': [774], 'past': [777], 'decade,': [778], 'microbial': [779], 'research': [780], 'has': [781, 2069, 3077], 'regained': [782], 'traction,': [783], 'particular': [786], 'focus': [787, 3085], 'microbiome': [791, 807], 'diversity': [792], 'importance': [795], 'health,': [797], 'difficult-to-treat': [800], 'infections': [801], 'multidrug-resistant': [803], 'pathogens.': [804], 'may': [808, 951, 2345], 'offer': [809], 'opportunities': [811], 'monitoring': [813], 'health': [814, 844], 'interventions': [816], 'long': [819], 'run': [820], '(Zeller': [821], 'et': [822, 826, 830, 850, 923, 927, 931, 939, 1013, 1028, 1043, 1047, 1062, 1130, 1134, 1138, 1142, 1179, 1184, 1188, 1192, 1227, 1339, 1343, 1347, 1408, 1634, 1642, 1709, 1717, 1989, 1993, 1997, 2001, 2011, 2064, 2079, 2101, 2388, 2392, 2472, 2493, 2578, 2582, 2603, 2666, 2674, 2690, 2826, 2830, 3035, 3435, 3670, 4052, 4145, 4149, 4180, 4184, 4207, 4242, 4270, 4274, 4278, 4282, 4286, 4338], 'al,': [823, 827, 831, 851, 924, 928, 932, 940, 1014, 1029, 1044, 1048, 1063, 1131, 1135, 1139, 1143, 1180, 1185, 1189, 1193, 1228, 1340, 1344, 1348, 1409, 1635, 1710, 1990, 1994, 1998, 2002, 2012, 2065, 2080, 2102, 2389, 2393, 2473, 2494, 2579, 2583, 2667, 2675, 2691, 2827, 2831, 3036, 3436, 3671, 4053, 4146, 4150, 4181, 4185, 4208, 4243, 4271, 4275, 4279, 4283, 4287, 4339], '2014;': [824, 1132, 1341, 1406, 2828], 'Bullman': [825], '2017;': [828], 'Maier': [829], '2018),': [832, 2584], 'shortage': [834], 'effective': [836], 'antibiotics': [837, 856, 903, 1258], 'poses': [838], 'an': [839, 909, 2447, 2504], 'imminent': [840], 'threat': [841], 'public': [843], '(Brown': [845], 'Wright,': [847], '2016;': [848, 1410, 4272, 4276, 4280, 4284], 'Tacconelli': [849], '2018).': [852, 1194, 3672], 'Discovery': [853], 'urgently': [858], 'needed,': [859], 'but': [860, 3093, 3212, 3531, 3726], 'developing': [861], 'lengthy,': [866], 'costly,': [867], 'unsuccessful': [870], 'process.': [871], 'Interestingly,': [872, 2632, 3956], 'bottlenecks': [877], 'early': [879], 'discovery': [880], 'stages': [881], 'remains': [882], 'lack': [884], 'tools': [886], 'identification': [888, 1125], 'mode': [891], 'action': [893], '(MoA)': [894], 'antibiotics.': [896], 'At': [897, 2186], 'same': [899, 3247, 3637, 3689, 3905, 4100], 'time,': [900], 'even': [901, 2383], 'decades,': [906], 'there': [907, 942], 'open': [910], 'debate': [911], 'how': [913, 947, 1297], 'leads': [916], 'death': [919], 'or': [920, 976, 1207, 1812, 1981, 2082, 2333, 2339, 2677, 2848, 3416, 3481, 3832, 3890, 4047, 4129, 4142], 'arrest': [921], '(Kohanski': [922], '2007;': [925, 2390], 'Ezraty': [926], '2013;': [929, 933, 937], 'Keren': [930], 'Liu': [934], 'Imlay,': [936], 'Dwyer': [938], '2014),': [941, 2013], 'little': [944], 'about': [946], 'much': [948, 2610], 'off-target': [949], 'effects': [950], 'play': [952], 'role': [954], 'MoA,': [957], 'our': [959, 1254, 1280, 2477, 2521, 2564, 2587], 'knowledge': [960], 'mechanisms': [963], 'incomplete.': [965], 'On': [966], 'other': [968, 2651, 2938, 3227, 3999], 'hand,': [969], 'physiology': [972, 1219], 'commensal,': [974], 'pathobiont,': [975], 'pathogenic': [977], 'equally': [980], 'important': [981], 'assessing': [983], 'microbiome-related': [984], 'questions': [985], 'devising': [987], 'intervention': [988], 'tools.': [989], 'recently': [992, 1056, 3663], 'introduced': [993, 1057], 'novel': [995, 1269, 3016], 'technology': [996], 'detecting': [998, 1148, 1157], 'protein–drug': [999, 1149], 'interactions': [1000, 1098, 1177], 'situ': [1002], 'proteome-wide': [1005, 1115], 'scale,': [1006], 'termed': [1007], '(TPP;': [1011], '2014).': [1015, 1050, 2403], 'combines': [1017], 'principle': [1019], 'shift': [1024, 3114], 'assay': [1025], '(Martinez': [1026], 'Molina': [1027], '2013)': [1030], 'multiplexed': [1032], 'quantitative': [1033], 'mass': [1034, 1039, 1497, 1631, 1706], 'spectrometry': [1035, 1632, 1707], '(MS)': [1036], 'using': [1037, 3381, 3567], 'tandem': [1038, 1496], 'tags': [1040], '(TMT;': [1041], 'McAlister': [1042], '2012;': [1045, 1999], 'Werner': [1046], '2012,': [1049], 'TPP,': [1052], 'sensitive': [1059, 2074], '2D-TPP': [1060], '(Becher': [1061], '2016),': [1064], 'cells': [1065, 1121, 1464, 1859, 2511, 2855, 3235, 3697], 'are': [1066, 1465, 1474, 1515, 1606, 2374, 2646, 2656, 3556, 3978, 4355], 'heated': [1067, 1475], 'range': [1070, 1160, 1358, 1478, 2179, 2206, 2250], 'temperatures': [1072, 1539, 1552, 1592, 1899, 1977, 2058, 2077, 2113, 3141], 'soluble': [1075, 1485], 'interrogated': [1081], 'quantitatively': [1082], 'each': [1084, 1510, 1518, 1531, 1654, 1729, 1779, 1887, 1924, 3772, 4331], 'temperature.': [1085], 'Therefore,': [1086], 'record': [1089], 'behavior': [1092, 1325, 1761, 3618, 3627, 3735, 3743, 3749, 3822, 4104, 4294, 4316], 'thousands': [1094], 'proteins.': [1096, 1525, 1747, 1881], 'Protein–drug': [1097], 'typically': [1099], 'increase': [1100, 2448, 3585, 3590], 'tolerance': [1103], 'proteins,': [1105, 1672, 2169, 2653, 2787, 3583], 'resulting': [1106], 'higher': [1108, 2163, 2699, 2963, 3417, 3607, 3737], 'apparent': [1109, 1933, 4234], 'points.': [1111], 'Thus,': [1112, 1195], 'comparison': [1113, 2275, 3699], 'drug-treated': [1118], 'drug-untreated': [1120], 'lead': [1123, 2725], '(Savitski': [1129, 1178, 1338, 2010, 2825], 'Franken': [1133, 1342], '2015;': [1136, 1140, 1186, 1345], 'Huber': [1137], 'Becher': [1141, 1187], '2016).': [1144], 'addition': [1146, 1690, 3274], 'interactions,': [1150, 1168, 1172, 1174, 1307], 'provides': [1152], 'powerful': [1154], 'tool': [1155], 'wide': [1159], 'state:': [1166], 'protein–metabolite': [1167], 'post-translational': [1169], 'modifications,': [1170], 'protein–protein': [1171], 'protein–DNA': [1173], 'chaperone–client': [1176], '2014,': [1181], '2018;': [1182, 1190], 'Reinhard': [1183, 1346, 2829], 'Tan': [1191], 'applying': [1196], 'growth/cell': [1200], 'cycle': [1201], 'stages,': [1202], 'nutritional': [1205], 'environments,': [1206], 'upon': [1208, 1689, 2097, 4078], 'chemical': [1209], 'perturbations': [1210], 'yield': [1212], 'unique': [1214, 1381, 3218, 3339, 4348], 'insight': [1215], 'underlying': [1222], 'adaptations': [1223], 'taking': [1224], 'place': [1225], '(Mateus': [1226], '2017).': [1229, 1636, 1711, 2003, 4054, 4244, 4288, 4340], 'reasoned': [1231], 'adapting': [1233], 'would': [1237, 1266, 3639], 'hold': [1238], 'great': [1239], 'promise': [1240], 'deconvoluting': [1242], 'MoA': [1244], 'compounds': [1247], 'activity': [1250, 2819, 2955, 3097, 3230, 3608], 'furthering': [1253], 'understanding': [1255, 1281], 'established': [1257], 'More': [1263], 'importantly,': [1264], 'it': [1265, 1298], 'provide': [1267], 'orthogonal': [1271], 'way': [1272], 'systematically': [1274], 'phenotyping': [1275], 'cell,': [1277], 'thereby': [1278], 'improving': [1279], 'basic': [1283], 'bacterial': [1284, 2247, 3728, 3961], 'biology.': [1285], 'Here,': [1286], 'adapt': [1288], 'apply': [1290], 'coli,': [1294], 'illustrate': [1296], 'gain': [1303, 3096], 'drug–protein': [1306], 'mechanisms,': [1309], 'formation.': [1315], 'Results': [1316], 'To': [1321, 2475, 3673], 'study': [1322], '(strain': [1330], 'BW25113)': [1331], 'proteome,': [1332, 2812], 'protocol': [1337, 1461], '2015)': [1349, 4209], 'optimizing': [1351], 'lysis': [1353], 'conditions': [1354], 'temperature': [1357, 1921, 2165, 2178, 2205, 2423, 2470, 3182, 3304], 'compatible': [1361], 'gram-negative': [1363], '(Fig': [1365, 1446, 1943, 2184, 2432, 2454, 3318, 3722, 3967, 4216], '1A;': [1366], 'see': [1367], '"Materials': [1368, 2896, 3184, 3390, 3701], 'Methods"': [1370], 'details).': [1372], 'identified': [1374, 1664, 1739, 1764, 1776, 1789, 1941, 2879], '1,831': [1375], '(with': [1377], 'least': [1379, 1385, 1662, 1737, 3216, 3222, 3332, 3337, 4346, 4352], 'two': [1380, 1386, 1663, 1738, 2712, 3223, 3285, 3333, 4347, 4353], 'peptides': [1382, 1652, 1665, 1727, 1740, 3070, 3219, 4349], 'replicates;': [1387], 'Fig': [1388, 1411, 1962, 2028, 2039, 2213, 2368, 2533, 2554, 2619, 2876, 2899, 2979, 3393, 4152, 4187, 4239, 4305], 'EV1A;': [1389], 'Dataset': [1390, 1448, 2234, 2535, 2621, 2901, 3395, 3704, 3724], 'EV1),': [1391], 'which': [1392, 1673, 1964, 2068, 2090, 2456, 2705, 2927, 2986, 3021, 3555, 3598, 4033, 4124, 4161, 4195, 4230, 4249], 'largely': [1393], 'overlapped': [1394], 'recent': [1396], 'proteomics': [1397, 1494, 1794, 3289], 'datasets': [1398, 1795], 'obtained': [1399, 1796], '(Wisniewski': [1403], 'Rakus,': [1405], 'Schmidt': [1407], 'EV1B).': [1412], 'confirmed': [1414], 'use': [1417], 'mild': [1420], 'detergent': [1421], '(NP-40)': [1422], 'did': [1423, 2199], 'not': [1424, 2200, 2639, 2679, 2947, 3198, 3313, 3342, 3524], 'affect': [1425], 'solubilization': [1427], 'extraction': [1429], 'membrane': [1431, 1880, 1891, 2228, 2257, 2264, 2286, 2355, 2371, 2442, 2467, 3931, 3986, 4075, 4082, 4226], 'proteins—as': [1432], 'no': [1433], 'bias': [1434], 'quantification': [1436], 'was': [1437, 1656, 1731, 1950, 2559, 3038, 3758, 4131, 4211, 4246], 'observed': [1438, 2006, 2408, 2538, 2960, 4114], 'when': [1439], 'compared': [1440], 'strong': [1443], 'detergent,': [1444], 'SDS': [1445], 'EV1C;': [1447], 'EV2).': [1449], 'Figure': [1450, 1758, 3127, 3740, 4313], '1.': [1451], 'overview.': [1462], 'After': [1463], 'grown': [1466], 'specified': [1469], 'optical': [1470], 'density': [1471], '(OD578),': [1472], 'aliquots': [1473], 'temperatures,': [1480], 'lysed,': [1481], 'remaining': [1484, 3652], 'fraction': [1486, 2419, 2426, 2435, 2751, 3799, 3861], 'collected.': [1491], 'Mass': [1492], 'spectrometry-based': [1493], '(using': [1495], 'tags,': [1498], 'TMT)': [1499], 'then': [1501], 'quantify': [1504], 'amount': [1506], 'condition,': [1511, 3203, 3211], 'curves': [1514, 1521, 1875, 2894, 3684, 3768, 3896, 4327], 'plotted': [1516, 1607], 'protein.': [1519, 2754], 'Melting': [1520, 1760, 1874, 3140, 3617, 3742, 3748, 3895, 4315], 'average': [1527, 1883, 2698, 3679, 3762], 'curve': [1529, 1885], 'shown.': [1535, 1894, 4356], 'Distribution': [1536, 1549, 1589, 1801, 1896], '(Tm)': [1540, 1553, 1593, 1900, 1936, 3142], 'proteomes.': [1548], 'proteomes': [1561], 'according': [1562, 1599, 1906], 'selected': [1564], 'gene': [1565], 'ontology': [1566], 'terms.': [1567], 'Line': [1568, 3774, 3836], 'represents': [1569, 1573, 3775, 3779, 3837, 3841, 4359], 'median,': [1571, 3777, 3839], 'box': [1572, 1581, 3778, 3787, 3840, 3849], 'interquartile': [1575, 3781, 3843], 'range,': [1576, 3782, 3844], 'whiskers': [1578, 3784, 3846], 'plots': [1582, 1605, 3788, 3850], 'represent': [1583, 1670, 1745, 3261, 3789, 3797, 3851, 3859], '5th': [1585], '95th': [1587], 'percentiles.': [1588, 3794, 3856], 'compartment.': [1603, 1910, 1926], 'Box': [1604], 'as': [1608, 1831, 2140, 2142, 2180, 2654, 2782, 2784, 2959, 3172, 3237, 3971, 4067], 'panel': [1609], '(D).': [1610], 'Correlation': [1611, 1680, 1817, 1853], 'points': [1614, 1624, 1683, 1699, 1935], 'lysate': [1616, 1685, 2507, 2522, 2588], 'determined': [1617, 1625, 1686, 1700, 2498, 3236], '(this': [1620, 1695], 'study)': [1621, 1696], 'limited': [1627, 1702, 2489, 2545], 'proteolysis': [1628, 1703, 2490, 2546], 'coupled': [1629, 1704], '(Leuenberger': [1633, 1708], 'For': [1637, 1712, 2851, 3579, 4016, 4116], 'results': [1639, 1714, 2478], 'Leuenberger': [1641, 1716, 2492, 2602], 'al': [1643, 1718, 2604], '(2017),': [1644, 1719], 'median': [1646, 1721], 'point': [1648, 1723, 1820, 1856, 1862], 'reported': [1651, 1726, 2462], 'used.': [1657, 1732], 'Only': [1658, 1733, 4341], 'were': [1666, 1741, 2216, 2357, 2638, 2905, 2928, 2943, 2999, 3055, 3196, 3366, 3376, 3399, 4233], 'compared.': [1667, 1742], 'Red': [1668, 1743], 'dots': [1669, 1744], 'ribosomal': [1671, 1746, 2172, 2567, 2652], 'generally': [1674, 2358, 4106], 'appear': [1675], 'less': [1676, 2561, 3568], 'TPP.': [1679], '10': [1692], 'mM': [1693], 'MgCl2': [1694, 2595], 'Download': [1748, 1750, 1927, 1929, 3269, 3271, 3952, 3954], 'figure': [1749, 1928, 3270, 3953], 'PowerPoint': [1751, 1930, 3272, 3955], 'Click': [1752, 4307], 'here': [1753, 4308], 'expand': [1755, 4310], 'figure.': [1757, 4312], 'EV1.': [1759], 'properties': [1772], 'A.': [1773, 3747], 'Reproducibility': [1774], 'replicate': [1780], 'analysis.': [1785], 'B.': [1786, 3817], 'Overlap': [1787], 'previously': [1792, 2005, 4113], 'published': [1793], 'coli.': [1799], 'C.': [1800, 3879], 'differences': [1803, 2709, 2816, 2884], 'between': [1804, 2520, 2710, 3682, 3765, 4321], 'abundance': [1806, 1824, 2021, 2799, 3163, 3418], 'after': [1807, 1985], 'being': [1808, 2427, 3343, 3448], 'extracted': [1809], 'NP-40': [1811], 'SDS.': [1814], 'D,': [1815], '(D)': [1822, 3908], '(r': [1825, 1846, 1867, 2022, 2033, 2527, 2548, 2613], '=': [1826, 1829, 1847, 1850, 1868, 2023, 2026, 2034, 2037, 2211, 2364, 2528, 2549, 2614, 2975, 3257], '0.06,': [1827, 2024], 'P': [1828, 1849, 1870, 2025, 2036, 2266, 2278, 2530, 2551, 2616, 3814, 3876, 3935], '0.015,': [1830], 'measured': [1832, 3173, 3759], 'top3': [1835, 3176], 'intensity': [1836, 3177], 'corresponding': [1837, 3178], 'lowest': [1840, 3181, 3303], 'temperature)': [1841], '(E)': [1843, 3912], 'molecular': [1844, 2031], 'weight': [1845, 2032], '−0.08,': [1848, 2035], '0.0009).': [1851], 'F.': [1852], 'living': [1858, 2524], 'lysate—both': [1864], '0.82,': [1869, 2529], '<': [1871, 1958, 2115, 2255, 2262, 2267, 2279, 2531, 2552, 2617, 2888, 3265, 3379, 3815, 3877], '0.0001).': [1872], 'G.': [1873], 'outer': [1879, 1890, 2227, 2263, 2352, 2441, 2466, 3930, 3985, 4081, 4225], 'class': [1888], 'H.': [1895], 'I.': [1911], 'Fraction': [1912], 'Tm': [1916, 2015, 2044, 2500, 2700, 3108], '>': [1917, 2301], '87°C': [1918, 2431], '(the': [1919], 'highest': [1920], 'tested)': [1922], 'calculated': [1932, 3677], '1,738': [1938], '1B).': [1944, 2433, 3319], 'Overall,': [1945], '(P': [1957, 2363, 2887, 2974], '0.0001,': [1959, 2268], 'Mann–Whitney': [1960, 2366], 'test;': [1961, 2367], '1C),': [1963], 'consistent': [1966, 2951, 3557, 4035, 4251], 'ability': [1969], 'organism': [1972], 'grow': [1974], 'regularly': [1975], 'up': [1978, 1982, 2429], '45°C': [1980], '49°C': [1984], 'evolutionary': [1986], 'adaptation': [1987], '(Fotadar': [1988], '2005;': [1991], 'Rudolph': [1992], '2010;': [1995], 'Blaby': [1996], 'Deatherage': [2000], 'As': [2004], 'correlated': [2016], 'very': [2017, 4025], 'weakly': [2018], '0.015;': [2027], 'EV1D)': [2029], '0.0009;': [2038], 'EV1E).': [2040], 'Proteins': [2041, 3153, 3187, 3195, 3397, 3413], 'low': [2043, 2057, 2112, 2779, 2789], 'included,': [2045], 'example,': [2047, 2833, 3973, 4017, 4117], 'TypA': [2048], '(BipA)': [2049], 'DeaD,': [2051], 'pivotal': [2052], '(Pfennig': [2059], 'Flower,': [2061], '2001;': [2062], 'Charollais': [2063], '2004);': [2066], 'GatZ,': [2067], 'been': [2070, 3078], 'described': [2071, 2600, 3529], 'high': [2076, 4257], '(Brinkkotter': [2078], '2002);': [2081], 'RNA': [2084], 'polymerase': [2085], 'sigma': [2086, 3421], 'D': [2087], 'factor,': [2088], 'RpoD,': [2089], 'lose': [2094], 'function': [2096], 'heat': [2098, 2514], 'shock': [2099], '(Blaszczak': [2100], '1995).': [2103], 'addition,': [2105, 3363], 'essential': [2107, 2786], 'also': [2109, 2944, 3000, 3039, 3532, 3603, 4136, 4173], '(Tm': [2114, 2300], '52°C),': [2116], 'topoisomerases': [2118], '(GyrB,': [2119], 'ParC,': [2120], 'ParE,': [2121], 'TopA),': [2122], 'involved': [2124, 3440, 3514], 'DNA': [2126, 3914, 3919, 4154, 4166], 'replication': [2127], '(DnaA,': [2128], 'DnaE)': [2129], 'shape': [2133], '(FtsA,': [2134], 'FtsE,': [2135], 'FtsI,': [2136], 'MrdA,': [2137], 'MreB,': [2138], 'MukB),': [2139], 'well': [2141, 2783], 'components': [2144, 3648, 4077, 4168], 'small': [2147, 2171, 3069], 'subunit': [2148, 2173, 2687, 4127], 'ribosome': [2151, 2558], '(RpsB,': [2152], 'RpsC,': [2153], 'RpsD,': [2154], 'RpsE,': [2155], 'RpsJ,': [2156], 'RpsL).': [2157], 'contrast': [2159], 'overall': [2162, 2626], 'similar': [2177, 2592, 3658], 'counterpart': [2183], '1D).': [2185], 'opposite': [2188], 'side': [2189], 'spectrum,': [2192], 'number': [2194], 'tested': [2204, 2801], '(up': [2207], '87°C,': [2209], 'n': [2210, 3256], '93;': [2212], 'EV1I);': [2214], 'these': [2215, 2904, 2941, 3582, 4007, 4118], 'enriched': [2217], 'Tat': [2220], 'translocation': [2221], 'system': [2222], '(TatA,': [2223], 'TatB,': [2224], 'TatE)': [2225], '(e.g.,': [2230], 'BamC,': [2231], 'OmpA,': [2232], 'TolC;': [2233], 'EV1).': [2235], 'Protein': [2236, 3162], 'interior': [2241], 'exterior': [2244], '(interquartile': [2249], 'Tm:': [2252], 'cytosol': [2253], '(53.3–59°C)': [2254], 'inner': [2256, 2285, 3981, 4074], '(55.5–61.7°C)': [2258], '≈': [2259, 2864, 2874], 'periplasm': [2260], '(54.5–61.2°C)': [2261], '(55.7–68.9°C);': [2265], 'Kruskal–Wallis': [2269], 'test,': [2270], "Dunn's": [2273], 'test': [2276, 3674], 'showing': [2277], '0.0001': [2280], 'all': [2282, 2304, 2335, 4073, 4296, 4322], 'comparisons': [2283], 'except': [2284], 'periplasm;': [2288], 'Figs': [2289], '1B': [2290], 'E,': [2292], 'EV1H).': [2294], 'most': [2296, 3580, 3957], 'stable': [2297, 2379, 2428, 4013], '65°C)': [2302], 'included': [2303, 3405, 3419, 3466, 4119], 'superoxide': [2306], 'dismutases': [2307], '(SodA,': [2308], 'SodB,': [2309], 'SodC),': [2310], 'chaperones': [2311], '(FkpA,': [2312], 'Skp,': [2313], 'Spy,': [2314], 'DsbC,': [2315], 'HdeA,': [2316], 'HdeB),': [2317], 'ligand-binding': [2319], 'ATP-binding': [2322], 'cassette': [2323], '(ABC)': [2324], 'transporters': [2325], '(such': [2326], 'as,': [2327], 'PotD,': [2328], 'PstS,': [2329], 'MlaD,': [2330], 'LolA,': [2331], 'DppA,': [2332], 'ArtI),': [2334], 'engaged': [2336], 'cargo': [2338], 'ligand': [2340], 'binding,': [2341], 'suggesting': [2342, 3063], 'capture': [2346], 'active': [2347, 3121], 'processes.': [2349], 'membrane,': [2353, 3982], 'integral': [2354, 2444], 'lipoproteins': [2362], '0.031,': [2365], 'EV1G).': [2369], 'Outer': [2370], 'porins': [2372], '(OMPs)': [2373], 'particularly': [2378], 'once': [2380], 'assembled': [2381], 'resistant': [2384], 'denaturation': [2386], '(Ureta': [2387], 'Burgess': [2391], '2008;': [2394, 2398], 'Stanley': [2395], 'Fleming,': [2397], 'Roman': [2399], 'Gonzalez': [2401], 'Flecha,': [2402], 'Compatible': [2404], 'this,': [2406, 2852, 3675], 'OMPs': [2411], 'showed': [2412, 2446, 2696], 'biphasic': [2414], 'behavior,': [2416, 3660], 'lower': [2422, 3600], 'another': [2425], 'A': [2434, 3706], '(mostly': [2443], 'proteins)': [2445], 'solubility': [2450], 'approximately': [2452], '55°C': [2453], 'EV1G),': [2455], 'could': [2457, 3279, 4105], 'linked': [2459], 'disorganization': [2463], '(Tsuchido': [2471], '1985).': [2474], 'compare': [2476], 'complementary': [2481], 'approach': [2482, 2547], 'unfolding': [2486, 2747], 'based': [2487], '(LiP-MS;': [2491], '2017),': [2495, 3037], 'further': [2497], '(by': [2508], 'lysing': [2509], 'prior': [2512], 'treatment).': [2515], 'Despite': [2516], 'good': [2518], 'agreement': [2519], 'experiments': [2526, 2589, 4268], '0.0001;': [2532, 2553, 2618], 'EV1F;': [2534], 'EV3),': [2536, 2622], 'only': [2539, 3008, 3354, 3525, 3594], 'moderate': [2541], 'correlation': [2542, 2612], '0.45,': [2550], '1F);': [2555], 'particularly,': [2556], 'considerably': [2560], 'experiments.': [2565], 'Since': [2566], 'affected': [2570, 2640, 3523], 'presence': [2573, 4046], 'magnesium': [2575, 2643], 'ions': [2576], '(Friedman': [2577], '1967;': [2580], 'Piazza': [2581], 'repeated': [2586], 'concentration': [2593], '(10': [2596], 'mM)': [2597], '(2017).': [2605], 'This': [2606, 2949, 3988], 'resulted': [2607], 'improved': [2611], '0.65,': [2615], '1G;': [2620], 'due': [2623], 'stability': [2628], 'ribosome.': [2631], 'RplA,': [2633], 'RplJ,': [2634], 'RplL,': [2635], 'RpsA': [2637], 'concentration.': [2644], 'These': [2645, 2714, 3465], 'structurally': [2647], 'distinct': [2648], 'they': [2655, 4029], 'part': [2657], 'lateral': [2660], 'stalk': [2661, 2671], '(RplJ': [2662], 'RplL;': [2664], 'Choi': [2665], '2015),': [2668], 'L1': [2670], '(RplA;': [2672], 'Reblova': [2673], '2012),': [2676], 'always': [2681], 'associated': [2683], '30S': [2686], '(RpsA;': [2688], 'Duval': [2689], '2013).': [2692], 'data': [2695], 'LiP-MS': [2703, 2722], 'approach,': [2704], 'probably': [2706], 'reflects': [2707], 'approaches.': [2713], 'include': [2715], 'technical': [2716], 'differences—a': [2717], 'longer': [2718], 'heat-treatment': [2719], 'time': [2720, 3248], 'might': [2724, 2744, 2835], 'extensive': [2728], 'unfolding—and': [2729], 'conceptual': [2730], 'differences—LiP-MS': [2731], 'detects': [2732], 'local': [2733], 'events': [2734], 'unfolding,': [2737], 'studies': [2740], 'aggregation': [2742], 'require': [2745], 'substantial': [2750], 'summary,': [2756, 3536], 'correlates': [2771], 'localization,': [2775], 'temperature-related': [2780], 'functions,': [2781], 'several': [2785], 'thermostability.': [2790], 'Impact': [2791, 3129], 'phase': [2794, 2806, 2862, 2872, 2935, 3030, 3100, 3132, 3328, 3373, 3404, 3433, 3612], 'impact': [2803], 'since': [2813, 3306, 3641], 'expected': [2815, 3632], 'reflected': [2822], '2015)—for': [2832], 'stabilized': [2837, 2929, 3001], 'substrates': [2839], '(indicating': [2840, 2846], 'flux': [2841], 'through': [2842, 3011], 'pathway),': [2844], 'products': [2845], 'inhibition),': [2847], 'allosteric': [2849], 'regulators.': [2850], 'harvested': [2854], 'growing': [2856], 'LB': [2858], 'medium': [2859], 'exponential': [2861, 3091, 3146, 3165, 3232, 3327], '(OD578': [2863, 2873], '0.1)': [2865], 'transition': [2869, 2932, 3027, 3059, 3148, 3167, 3358, 3370, 3549], 'stationary': [2871, 2934, 2957, 3029, 3061, 3099, 3150, 3169, 3234, 3349, 3360, 3372, 3432, 3551], '2;': [2875], '1A).': [2877], '39': [2880], 'significant': [2883], '0.001,': [2889], 'non-parametric': [2890], 'analysis': [2891], 'response': [2893], '(see': [2895, 3183, 3389, 3700, 4085], 'Methods";': [2898, 3703], '2A;': [2900], 'EV4).': [2902], 'Among': [2903], 'lactate': [2906], 'dehydrogenase': [2907], '(LldD),': [2908], 'some': [2910], 'members': [2913, 2939, 4001, 4065], 'respiratory': [2916, 2954, 3510], 'I': [2918], '(NuoA': [2919], 'NuoL)': [2921], 'II': [2923], '(SdhB': [2924], 'SdhD),': [2926], '(all': [2936], 'stabilized,': [2945], 'albeit': [2946], 'significantly).': [2948], 'stronger': [2953], 'phase,': [2958, 3062, 3092, 3552], 'triphenylformazan': [2966, 3244], 'formed': [2967], 'reduction': [2970], 'triphenyltetrazolium': [2972, 3241], 'chloride': [2973, 3242], '0.0027,': [2976], "Student's": [2977, 3267], 't-test;': [2978], '2C).': [2980], 'Accordingly,': [2981], 'glycerol': [2983, 2988, 3006, 3495], 'kinase': [2984], '(GlpK),': [2985], 'converts': [2987], 'sn-glycerol': [2990, 2994], '3-phosphate,': [2991], '3-phosphate': [2995], 'uptake': [2996], 'transporter': [2997, 3018, 3410], '(GlpT)': [2998], 'phase;': [3005], 'respiration.': [3012], 'Furthermore,': [3013], 'YjiY,': [3014], 'specific': [3017], 'pyruvate,': [3020, 3518], 'induced': [3023], 'exactly': [3024], 'import': [3032], 'pyruvate': [3033], '(Kristoficova': [3034], 'phase.': [3044, 3361], 'Conversely,': [3045], 'peptide': [3047], 'transporters,': [3048], 'MppA': [3049], 'OppA,': [3051], 'tryptophanase': [3053], '(TnaA)': [3054], 'destabilized': [3056], 'exhausted': [3068], 'started': [3073, 3315], 'producing': [3074], 'indole.': [3075], 'TnaA': [3076], 'shown': [3079], 'form': [3081, 4030, 4200], 'single': [3083, 3829, 3887], 'inactive': [3084], 'pole': [3089], 'during': [3090, 3245, 3547], 'disperse': [3094], '(Li': [3101, 3123, 3613], 'Young,': [3103, 3125, 3615], '2015).': [3104, 3126, 3616], 'decrease': [3106], 'presumably': [3110, 4004], 'reflective': [3111], 'focal': [3117], 'conformation': [3118], 'free': [3120], 'tetramers': [3122], '2.': [3128], 'phases.': [3152], 'highlighted': [3154, 3188], 'orange': [3156, 3190], 'indicate': [3157, 3191], 'significantly': [3158, 3192], 'behavior.': [3161], 'phases,': [3171], 'Methods").': [3186], 'levels.': [3194], 'considered': [3197], 'detectable': [3199, 3213, 3344], '(n.d.)': [3200], 'if': [3204], 'absent': [3205, 3400], 'three': [3207, 3217, 3338, 3346, 4231], 'replicates': [3208, 3224, 3334, 3347, 4354], 'condition.': [3228], 'Respiratory': [3229], 'conversion': [3239], 'normalized': [3250], 'OD': [3252], '(˜number': [3253], 'cells).': [3255], '3;': [3258], 'error': [3259], 'bars': [3260], 'standard': [3262], 'deviation;': [3263], '**P': [3264], '0.01,': [3266, 3380], 't-test.': [3268], 'thermostability,': [3277, 3601], 'estimate': [3280], 'abundances': [3282], 'phases—as': [3287], 'traditional': [3288], 'approaches': [3290], '(Aebersold': [3291], 'Mann,': [3293], '2016)—by': [3294], 'comparing': [3295], 'intensities': [3297], 'signal': [3300], '(37°C),': [3305], 'vast': [3308], 'majority': [3309, 3445], 'yet': [3314], 'found': [3321], '17': [3322], 'exclusively': [3324], 'expressed': [3325, 3355], '(identified': [3329], 'peptides,': [3340], 'phase)': [3350], '57': [3352], 'five': [3364], 'down-regulated': [3367], '53': [3375], 'up-regulated': [3377], '[P': [3378], 'linear': [3383], 'model': [3384], 'assess': [3386], 'expression': [3388], 'Methods");': [3392], '2B;': [3394], 'EV4].': [3396], 'iron': [3407], '(III)': [3408], 'hydroxamate': [3409], '(FhuC,': [3411], 'FhuD).': [3412], 'exclusive': [3415], 'factor': [3422], 'S': [3423], '(RpoS)—note': [3424], 'regulon': [3427], 'activated': [3429], 'later': [3430], '(Weber': [3434], '2005)—and': [3437], 'processes,': [3443], 'them': [3447, 3655], 'regulated': [3449], 'cAMP': [3451], 'receptor': [3452], '(CRP)—known': [3454], 'dominant': [3457], 'stage': [3461], 'rich': [3463], 'media.': [3464], 'mediating': [3468], 'fatty': [3469], 'acid': [3470], 'beta-oxidation': [3471], '(FadA,': [3472], 'FadI,': [3473], 'FadJ);': [3474], 'transport': [3476], 'maltose': [3478], '(MalF,': [3479], 'MalK)': [3480], 'sorbitol': [3482], '(SrlA,': [3483], 'SrlB,': [3484], 'SrlD,': [3485], 'SrlE);': [3487], 'catabolism': [3490], 'threonine': [3492], '(TdcB,': [3493], 'TdcE),': [3494], '(GlpB,': [3496], 'GlpC),': [3497], 'glucarate': [3498], '(GarL,': [3499], 'GudD),': [3500], 'N-acetylneuraminate': [3501], '(NanE,': [3502], 'NanK),': [3503], 'glycine': [3505], '(GcvP,': [3506], 'GcvT).': [3507], 'Members': [3508], 'glycerol,': [3516], 'lactate,': [3517], 'tryptophan': [3520], 'metabolism': [3521], '(as': [3528], 'above),': [3530], 'abundance.': [3534, 3592], 'observe': [3538], 'many': [3542, 3553, 4005], 'enzymes': [3544], 'efficient': [3569], 'energy': [3570], 'sources': [3571], 'slowing': [3573], 'down': [3574], 'stage.': [3578], 'coincided': [3588], 'prominent': [3595], 'outlier,': [3596], 'TnaA,': [3597], 'Next,': [3622], 'evaluated': [3624], 'complexes.': [3630], 'co-melt,': [3640], 'destabilize': [3650], 'members—causing': [3654], 'phenomenon': [3662], 'called': [3664], 'proximity': [3666], 'coaggregation,': [3667], 'TPCA': [3668], '(Tan': [3669], 'Euclidean': [3680, 3738, 3763], 'distance': [3681, 3764, 4320], 'EV5).': [3705], 'large': [3707], 'proportion': [3708], 'coherently': [3713, 3805, 3867, 3943], 'both': [3715], '3A;': [3723], 'EV6),': [3725], 'variable': [3733], '(i.e.,': [3736], 'distance).': [3739], '3.': [3741], 'complex.': [3773, 3934], '10th': [3791, 3853], '90th': [3793, 3855], 'Pie': [3795, 3857], 'charts': [3796, 3858], '(compared': [3806, 3868, 3944], 'distribution': [3809, 3871, 3947], '10,000': [3811, 3873, 3949], 'random': [3812, 3874, 3950], 'complexes;': [3813, 3875], '0.05).': [3816, 3878], 'Comparison': [3818], 'located': [3826, 3884, 3902], 'compartments.': [3835, 3893], 'Schematic': [3880], 'representation': [3881], 'D–H.': [3894], 'examples': [3898], 'non-co-melting': [3900, 3960, 4103], 'compartment:': [3907], 'ClpP': [3909], 'protease': [3910], 'complex,': [3911, 3916, 3921, 3926, 4193, 4221], 'Ruv': [3913], 'repair': [3915, 3920, 4155], '(F)': [3917], 'Uvr': [3918], '(G)': [3922], 'Suf': [3923, 4192], 'Fe-S': [3924], 'biogenesis': [3925], '(H)': [3928], 'Bam': [3929, 4220], 'assembly': [3933], 'indicates': [3936], 'probability': [3938], 'melts': [3942], 'complexes).': [3951], 'spanned': [3963], '3B': [3968], 'C),': [3970], 'multidrug': [3974], 'composed': [3979], 'periplasmic,': [3983], 'components.': [3987], 'suggests': [3989], 'localization': [3992, 4039], 'overrides': [3993], 'effect': [3995], 'stabilization': [3997], 'across-compartment': [4008], 'consist': [4010], 'co-localized': [4014], 'subcomplexes.': [4015, 4115], 'inner-membrane-bound': [4019], 'pumps': [4023], 'similarly': [4026], 'indicating': [4027], 'separate': [4031], 'subcomplexes,': [4032], 'dynamics': [4041], 'AcrB': [4043], 'absence': [4048], 'TolC': [4050, 4084], '(Bergmiller': [4051], 'Nevertheless,': [4055], 'such': [4056], 'still': [4059], 'linked,': [4066], 'evidenced': [4068], 'removing': [4079], 'counterpart,': [4083], 'below': [4086], '"Effect': [4087], 'knocking': [4089], 'out': [4090], 'abundance").': [4096], 'Complexes': [4097], 'attributed': [4108], 'formation': [4111], 'following:': [4121], 'proteases,': [4122], 'catalytic': [4126], '(ClpP': [4128], 'HslV)': [4130], 'singly': [4137], 'acting': [4138], 'chaperonin': [4139], '(ClpA,': [4140], 'ClpX,': [4141], 'HslU;': [4143], 'Rohrwild': [4144], '1996;': [4147], 'Ortega': [4148], '2004;': [4151], '3D);': [4153], '(Ruv': [4157], 'Uvr),': [4159], 'respective': [4163], 'binding': [4167], '(RuvA': [4169], 'UvrA)': [4171], 'act': [4174], 'own': [4177], '(Van': [4178], 'Houten': [4179], '1987;': [4182], 'Shiba': [4183], '1991;': [4186], '3E': [4188], 'F);': [4190], 'SufBD': [4196], 'core': [4202], '(Hirabayashi': [4206], 'SufC': [4215], '3G);': [4217], 'machinery': [4223], 'insertion,': [4228], 'subcomplexes': [4232], '(BamAB,': [4235], 'BamDE,': [4236], 'BamC;': [4238], '3H;': [4240], 'Noinaj': [4241, 4285], 'BamC': [4245], 'completely': [4247], 'thermostable,': [4248], 'enigmatic': [4254], 'topology,': [4255], 'i.e.,': [4256], 'flexibility': [4258], 'crystal': [4260], 'structures': [4261], 'appearing': [4263], 'surface-exposed': [4264], '(Bakelar': [4269], 'Gu': [4273], 'Han': [4277], 'Iadanza': [4281], 'An': [4289], 'overview': [4290], 'co-melting': [4293], 'detected': [4301, 4343], 'available': [4303], 'EV2.': [4306, 4314], 'complexesEuclidean': [4319], 'pairs': [4324], 'complex—collected': [4332], 'EcoCyc': [4334], 'v.21.1': [4335], '(https://ecocyc.org/;': [4336], 'Keseler': [4337], 'Node': [4357], 'color': [4358], 'location,': [4361], 'edge': [4363], 'c': [4364]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2873517389', 'counts_by_year': [{'year': 2024, 'cited_by_count': 31}, {'year': 2023, 'cited_by_count': 16}, {'year': 2022, 'cited_by_count': 28}, {'year': 2021, 'cited_by_count': 24}, {'year': 2020, 'cited_by_count': 32}, {'year': 2019, 'cited_by_count': 21}, {'year': 2018, 'cited_by_count': 3}], 'updated_date': '2025-01-09T10:33:12.355295', 'created_date': '2018-07-19'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
