Abstract:Two separate and distinct steps are involved in chemoautotrophic nitrification. In the first, ammonium is oxidized to nitrite by ammonium-oxidizing bacteria, or broadly speaking, the Nitrosomonas grou...Two separate and distinct steps are involved in chemoautotrophic nitrification. In the first, ammonium is oxidized to nitrite by ammonium-oxidizing bacteria, or broadly speaking, the Nitrosomonas group. In the second, nitrite is oxidized to nitrate by the Nitrobacter group. This chapter considers determination of the ammonium-oxidizing and the nitrite-oxidizing bacteria, and the terms Nitrosomonas and Nitrobacter, respectively, are used interchangeably with the two groups. Methods are described for enumeration of Nitrosomonas and Nitrobacter by the most-probable-number (MPN) method and for isolation of the two groups in pure culture. In the case of Nitrosomonas, dilutions of soil are inoculated into an inorganic medium containing ammonium as the source of nitrogen. If Nitrosomonas is present in viable form in the inoculum, growth will occur, and nitrite will be produced. To enumerate and isolate the Nitrobacter group, the medium employed is free of organic materials and contains nitrite as an energy source.Read More
Publication Year: 2016
Publication Date: 2016-05-13
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 70
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot