Title: INVITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Carica papaya AND Azadirachta indica LEAF AND STEM BARK EXTRACTS ON SELECTED CLINICAL ISOLATES
Abstract: The search for alternative sources of antibiotic is a global challenge due to the increase in the
emergence of resistant strams. Plants have been in use in traditional medicine before the era of
chemotherapeutics and about 80% of the global population still uses them . . Azadirachta indica
(neem) and Carica papaya are trees that have been found to possess antibacterial, antifungal, antiinflammatory,
anti-tumour properties and also used as a pest icide. In this work, antibacterial,
phytochemical and antioxidant potentials of ethanolic and aqueous extracts of Carica papaya and
Azadirachta indica leaf and stem bark was determined using antimicrobial sensitivity assay,
minimum inhibitory concentration, minimum lethal concentration, Ferric reducing antioxidant
power assay and Total antioxidant activity of extracts as indices. The test organisms were
Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus au reus. Azadirachta
indica leaf water extract and Azadirachta indica stem bark ethanol extract showed a clear zone of
inhibition ranging from 10±0mm to 15.5±0.71mm and 10±0mm to 15.5±2.12mm respectively
against all four test isolates, while others extracts had clear zones of inhibition against at least three
test isolates with inhibition zones ranging from 10.5±0. 71mm to 15±1.41mm. Ethanolic extract of
Carica papaya leaf was active against Bacillus subtilis alone (11.5±0.71mm).Some combined
extracts expressed activity against all four isolate, while the highest individual extract inhibition
zone was 15.5±2.12mm, combined extract was 18.5±0. 71mm against Salmonella typhimurium. All
extracts had antioxidant activity and some of the phytochemicals present in the extracts include
saponins, flavonoids, tannins, anthocyanin, betacyanin, quinones, cardiac glycoside, terpenoids,
and phenols. However further research is still needed to identify the active phytochemica/s and their
concentrations in the extracts
Publication Year: 2017
Publication Date: 2017-01-01
Language: en
Type: article
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