Title: Characterization of Botrytis cinerea isolates fromchickpea: DNA polymorphisms, cultural, morphologicaland virulence characteristics
Abstract: Eight isolates of Botrytis cinerea, causal organism of chickpea from eight different locations of western
and eastern Indo-Gangetic Plains (IGP) of India were analyzed for morphological, cultural, virulence and
genetic variations. Characterization of virulence and genetic variations of the isolates was based on
their pathogenicity against 40 selected chickpea genotypes and randomly amplified polymorphic DNA
(RAPD) analysis, respectively. The isolates differed in their optimum growth, temperature, conidial
formation and size of conidia on potato dextrose agar. Based on cluster analysis, the eight test isolates
were separated into three pathotypes with two isolates from western and four from eastern IGP grouped
together with >80% similarity. Based on cluster analysis of the RAPD banding patterns, genetic
similarity of the isolates varied from 14-44%, and the isolates were separated into three groups.
However, pathotypes variation detected among B. cinerea isolates could not be differentiated based on
the RAPD markers examined
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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