Publication Information

{'id': 'https://openalex.org/W2790198776', 'doi': 'https://doi.org/10.15252/embj.201797825', 'title': 'Transmembrane redox control and proteolysis of PdeC, a novel type of c‐di‐ <scp>GMP</scp> phosphodiesterase', 'display_name': 'Transmembrane redox control and proteolysis of PdeC, a novel type of c‐di‐ <scp>GMP</scp> phosphodiesterase', 'publication_year': 2018, 'publication_date': '2018-03-07', 'ids': {'openalex': 'https://openalex.org/W2790198776', 'doi': 'https://doi.org/10.15252/embj.201797825', 'mag': '2790198776', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/29514851', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/5897775'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/embj.201797825', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S127916151', 'display_name': 'The EMBO Journal', 'issn_l': '0261-4189', 'issn': ['0261-4189', '1460-2075'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'https://doi.org/10.15252/embj.201797825', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5103195829', 'display_name': 'Susanne Herbst', 'orcid': 'https://orcid.org/0000-0003-0938-6870'}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Susanne Herbst', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5003613927', 'display_name': 'Martin Lorkowski', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Martin Lorkowski', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5021853420', 'display_name': 'Olga Sarenko', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Olga Sarenko', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5073176293', 'display_name': 'Thi Kim Loan Nguyen', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Thi Kim Loan Nguyen', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5050017483', 'display_name': 'Tina Jaenicke', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Tina Jaenicke', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5018564313', 'display_name': 'Regine Hengge', 'orcid': 'https://orcid.org/0000-0002-3969-8018'}, 'institutions': [{'id': 'https://openalex.org/I39343248', 'display_name': 'Humboldt-Universität zu Berlin', 'ror': 'https://ror.org/01hcx6992', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I39343248']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Regine Hengge', 'raw_affiliation_strings': ['Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Biologie/Mikrobiologie Humboldt‐Universität zu Berlin Berlin Germany', 'institution_ids': ['https://openalex.org/I39343248']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 0, 'currency': 'USD', 'value_usd': 0, 'provenance': 'doaj'}, 'apc_paid': {'value': 0, 'currency': 'USD', 'value_usd': 0, 'provenance': 'doaj'}, 'fwci': 1.839, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 39, 'citation_normalized_percentile': {'value': 0.999983, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 95, 'max': 96}, 'biblio': {'volume': '37', 'issue': '8', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10593', 'display_name': 'Bacterial biofilms and quorum sensing', 'score': 0.9887, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10593', 'display_name': 'Bacterial biofilms and quorum sensing', 'score': 0.9887, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12891', 'display_name': 'Medicinal Plants and Neuroprotection', 'score': 0.9872, 'subfield': {'id': 'https://openalex.org/subfields/2707', 'display_name': 'Complementary and alternative medicine'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12023', 'display_name': 'Cholinesterase and Neurodegenerative Diseases', 'score': 0.9863, 'subfield': {'id': 'https://openalex.org/subfields/2736', 'display_name': 'Pharmacology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/proteolysis', 'display_name': 'Proteolysis', 'score': 0.85850143}], 'concepts': [{'id': 'https://openalex.org/C2781307694', 'wikidata': 'https://www.wikidata.org/wiki/Q33123', 'display_name': 'Proteolysis', 'level': 3, 'score': 0.85850143}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.819309}, {'id': 'https://openalex.org/C62826618', 'wikidata': 'https://www.wikidata.org/wiki/Q424304', 'display_name': 'Phosphodiesterase', 'level': 3, 'score': 0.5953076}, {'id': 'https://openalex.org/C24530287', 'wikidata': 'https://www.wikidata.org/wiki/Q424204', 'display_name': 'Transmembrane protein', 'level': 3, 'score': 0.58782315}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.5402718}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.32921588}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.23599279}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.17161646}], 'mesh': [{'descriptor_ui': 'D048168', 'descriptor_name': 'Escherichia coli K12', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D010727', 'descriptor_name': 'Phosphoric Diester Hydrolases', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D018441', 'descriptor_name': 'Biofilms', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006152', 'descriptor_name': 'Cyclic GMP', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D006152', 'descriptor_name': 'Cyclic GMP', 'qualifier_ui': 'Q000031', 'qualifier_name': 'analogs & derivatives', 'is_major_topic': False}, {'descriptor_ui': 'D006152', 'descriptor_name': 'Cyclic GMP', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D048168', 'descriptor_name': 'Escherichia coli K12', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010084', 'descriptor_name': 'Oxidation-Reduction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010727', 'descriptor_name': 'Phosphoric Diester Hydrolases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000072417', 'descriptor_name': 'Protein Domains', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D059748', 'descriptor_name': 'Proteolysis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 5, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/embj.201797825', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S127916151', 'display_name': 'The EMBO Journal', 'issn_l': '0261-4189', 'issn': ['0261-4189', '1460-2075'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc5897775', 'pdf_url': 'https://europepmc.org/articles/pmc5897775?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897775', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'http://edoc.hu-berlin.de/18452/22939', 'pdf_url': 'https://edoc.hu-berlin.de/bitstream/18452/22939/3/dissertation_%20lorkowski_martin.pdf', 'source': {'id': 'https://openalex.org/S4306400923', 'display_name': 'edoc Publication server (Humboldt University of Berlin)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I39343248', 'host_organization_name': 'Humboldt-Universität zu Berlin', 'host_organization_lineage': ['https://openalex.org/I39343248'], 'host_organization_lineage_names': ['Humboldt-Universität zu Berlin'], 'type': 'repository'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/29514851', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.15252/embj.201797825', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S127916151', 'display_name': 'The EMBO Journal', 'issn_l': '0261-4189', 'issn': ['0261-4189', '1460-2075'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319965', 'host_organization_name': 'Springer Nature', 'host_organization_lineage': ['https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Springer Nature'], 'type': 'journal'}, 'license': 'cc-by-nc-nd', 'license_id': 'https://openalex.org/licenses/cc-by-nc-nd', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'score': 0.46, 'id': 'https://metadata.un.org/sdg/6', 'display_name': 'Clean water and sanitation'}], 'grants': [{'funder': 'https://openalex.org/F4320320879', 'funder_display_name': 'Deutsche Forschungsgemeinschaft', 'award_id': 'He 1556/17‐1'}, {'funder': 'https://openalex.org/F4320320879', 'funder_display_name': 'Deutsche Forschungsgemeinschaft', 'award_id': 'He 1556/21‐1'}, {'funder': 'https://openalex.org/F4320338335', 'funder_display_name': 'H2020 European Research Council', 'award_id': 'ERC‐AdG 249780'}], 'datasets': [], 'versions': [], 'referenced_works_count': 56, 'referenced_works': ['https://openalex.org/W1557428043', 'https://openalex.org/W1598629441', 'https://openalex.org/W1599420007', 'https://openalex.org/W1842915559', 'https://openalex.org/W1863386058', 'https://openalex.org/W1953071049', 'https://openalex.org/W1989685072', 'https://openalex.org/W1991658240', 'https://openalex.org/W1995445964', 'https://openalex.org/W1997355092', 'https://openalex.org/W2002049404', 'https://openalex.org/W2002537715', 'https://openalex.org/W2003729219', 'https://openalex.org/W2004362709', 'https://openalex.org/W2006823831', 'https://openalex.org/W2009189380', 'https://openalex.org/W2014843464', 'https://openalex.org/W2025628802', 'https://openalex.org/W2033797992', 'https://openalex.org/W2047670590', 'https://openalex.org/W2047930189', 'https://openalex.org/W2048511647', 'https://openalex.org/W2053458854', 'https://openalex.org/W2053460725', 'https://openalex.org/W2054799851', 'https://openalex.org/W2063868052', 'https://openalex.org/W2064080654', 'https://openalex.org/W2064587709', 'https://openalex.org/W2064923905', 'https://openalex.org/W2068004712', 'https://openalex.org/W2086323657', 'https://openalex.org/W2087616530', 'https://openalex.org/W2094847059', 'https://openalex.org/W2096209101', 'https://openalex.org/W2104767186', 'https://openalex.org/W2105972088', 'https://openalex.org/W2109335319', 'https://openalex.org/W2117445618', 'https://openalex.org/W2121803750', 'https://openalex.org/W2122836009', 'https://openalex.org/W2132665880', 'https://openalex.org/W2144516903', 'https://openalex.org/W2155581972', 'https://openalex.org/W2156752198', 'https://openalex.org/W2158345344', 'https://openalex.org/W2160295639', 'https://openalex.org/W2160682526', 'https://openalex.org/W2168421163', 'https://openalex.org/W2171132114', 'https://openalex.org/W2209062912', 'https://openalex.org/W2280283366', 'https://openalex.org/W2524698591', 'https://openalex.org/W2586995851', 'https://openalex.org/W2754500289', 'https://openalex.org/W2762905984', 'https://openalex.org/W783470056'], 'related_works': ['https://openalex.org/W614643624', 'https://openalex.org/W251682942', 'https://openalex.org/W2411886259', 'https://openalex.org/W2411030048', 'https://openalex.org/W2367939396', 'https://openalex.org/W2363211455', 'https://openalex.org/W2334475454', 'https://openalex.org/W2057158614', 'https://openalex.org/W2019386382', 'https://openalex.org/W1500086673'], 'abstract_inverted_index': {'Article7': [0], 'March': [1], '2018Open': [2], 'Access': [3], 'Source': [4, 2293, 2301, 2827, 2835, 3587, 3595, 4285, 4293], 'DataTransparent': [5], 'process': [6, 323], 'Transmembrane': [7], 'redox': [8, 484, 619, 1182, 1192, 1352, 1385, 1470, 2408], 'control': [9, 485, 620, 775, 1057, 1160, 1395, 1445, 1480, 1757, 2173, 2423], 'and': [10, 282, 312, 330, 361, 402, 404, 466, 486, 507, 532, 554, 589, 609, 621, 708, 738, 760, 801, 840, 853, 864, 893, 929, 946, 988, 1025, 1044, 1049, 1070, 1094, 1115, 1238, 1277, 1315, 1391, 1433, 1472, 1547, 1588, 1604, 1634, 1642, 1653, 1665, 1734, 1943, 1969, 2084, 2109, 2132, 2137, 2233, 2258, 2263, 2317, 2415, 2700, 2707, 2740, 2795, 2815, 2819, 2889, 2901, 2963, 2978, 3025, 3033, 3085, 3096, 3115, 3125, 3130, 3135, 3227, 3336, 3379, 3400, 3461, 3472, 3503, 3505, 3528, 3720, 3738, 3774, 3793, 3806, 3856, 3950, 4021, 4061, 4089, 4335, 4417], 'proteolysis': [11, 487, 622, 2515, 2851, 3134], 'of': [12, 17, 309, 319, 377, 417, 423, 510, 518, 548, 581, 600, 642, 663, 669, 677, 725, 757, 786, 791, 827, 879, 888, 915, 1002, 1021, 1040, 1068, 1073, 1076, 1078, 1101, 1110, 1121, 1134, 1138, 1164, 1246, 1274, 1291, 1342, 1354, 1371, 1376, 1387, 1398, 1408, 1414, 1422, 1427, 1446, 1458, 1482, 1493, 1505, 1513, 1516, 1534, 1544, 1552, 1555, 1586, 1613, 1630, 1686, 1758, 1760, 1808, 1844, 1876, 1897, 1908, 1966, 2033, 2077, 2086, 2135, 2144, 2156, 2161, 2174, 2213, 2215, 2223, 2249, 2256, 2314, 2319, 2329, 2336, 2349, 2384, 2397, 2402, 2410, 2444, 2462, 2495, 2507, 2528, 2536, 2560, 2653, 2665, 2673, 2680, 2688, 2738, 2765, 2783, 2873, 2907, 2950, 3000, 3015, 3031, 3080, 3100, 3113, 3120, 3138, 3143, 3150, 3170, 3184, 3202, 3243, 3251, 3255, 3265, 3303, 3338, 3384, 3404, 3428, 3451, 3479, 3490, 3493, 3545, 3550, 3554, 3559, 3569, 3585, 3607, 3609, 3630, 3642, 3646, 3652, 3680, 3686, 3714, 3718, 3733, 3846, 3880, 3890, 3983, 4009, 4023, 4058, 4085, 4092, 4096, 4110, 4139, 4158, 4169, 4178, 4198, 4224, 4238, 4241, 4305, 4339, 4368, 4379, 4405, 4415, 4422, 4426, 4449, 4457], 'PdeC,': [13, 415, 1418, 1494, 2330, 2766, 3480], 'a': [14, 317, 374, 385, 407, 469, 492, 650, 673, 726, 825, 921, 977, 1074, 1118, 1139, 1304, 1308, 1420, 1463, 1582, 1590, 1600, 1605, 1611, 1702, 1761, 1769, 1781, 1815, 1850, 1963, 2091, 2227, 2325, 2343, 2355, 2416, 2433, 2460, 2486, 2502, 2521, 2537, 2589, 2604, 2666, 2683, 2758, 2767, 2956, 2983, 3048, 3081, 3107, 3195, 3200, 3220, 3285, 3293, 3309, 3321, 3349, 3431, 3445, 3485, 3672, 3702, 3723, 3777, 3786, 3894, 3904, 3917, 3923, 4035, 4147, 4172, 4175, 4190, 4206, 4270, 4376, 4442], 'novel': [15, 375, 3158], 'type': [16, 1100, 1426, 1554], 'c-di-GMP': [18, 350, 363, 380, 528, 544, 551, 720, 880, 1428, 1562, 1623, 1986, 2374, 2385], 'phosphodiesterase': [19, 628], 'Susanne': [20, 22, 144, 146, 270], 'Herbst': [21, 23, 145, 147], 'orcid.org/0000-0003-0938-6870': [24, 148], 'Institut': [25, 44, 63, 86, 105, 129, 149, 168, 187, 210, 229, 253], 'für': [26, 45, 64, 87, 106, 130, 150, 169, 188, 211, 230, 254, 287], 'Biologie/Mikrobiologie,': [27, 46, 65, 88, 107, 131, 151, 170, 189, 212, 231, 255, 288], 'Humboldt-Universität': [28, 47, 66, 89, 108, 132, 152, 171, 190, 213, 232, 256, 289], 'zu': [29, 48, 67, 90, 109, 133, 153, 172, 191, 214, 233, 257, 290], 'Berlin,': [30, 31, 49, 50, 68, 69, 91, 92, 110, 111, 134, 135, 154, 155, 173, 174, 192, 193, 215, 216, 234, 235, 258, 259, 291, 292], 'Germany': [32, 51, 70, 93, 112, 136, 156, 175, 194, 217, 236, 260, 293], 'Search': [33, 52, 71, 94, 113, 137, 157, 176, 195, 218, 237, 261], 'for': [34, 53, 72, 95, 114, 138, 158, 177, 196, 219, 238, 262, 491, 625, 960, 1465, 1540, 2203, 2298, 2303, 2418, 2424, 2750, 2760, 2832, 2837, 3426, 3476, 3487, 3581, 3592, 3597, 3639, 3711, 3823, 3907, 3967, 4082, 4132, 4233, 4253, 4290, 4295, 4332, 4430], 'more': [35, 54, 73, 96, 115, 139, 159, 178, 197, 220, 239, 263, 704, 851, 855, 3401], 'papers': [36, 55, 74, 97, 116, 140, 160, 179, 198, 221, 240, 264], 'by': [37, 56, 75, 98, 117, 141, 161, 180, 199, 222, 241, 265, 366, 397, 406, 462, 480, 541, 558, 592, 731, 740, 1212, 1252, 1259, 1271, 1561, 1819, 1901, 1994, 2023, 2070, 2124, 2276, 2376, 2447, 2531, 2629, 2725, 2825, 2911, 3006, 3092, 3332, 3534, 4219], 'this': [38, 57, 76, 99, 118, 142, 162, 181, 200, 223, 242, 266, 458, 1015, 1055, 1154, 1292, 1324, 1423, 1553, 2299, 2508, 2595, 2833, 2850, 2879, 3263, 3345, 3593, 3971, 4291], 'author': [39, 58, 77, 100, 119, 143, 163, 182, 201, 224, 243, 267, 331], 'Martin': [40, 42, 164, 166, 272], 'Lorkowski': [41, 43, 165, 167], 'Olga': [59, 61, 183, 185, 274], 'Sarenko': [60, 62, 184, 186], 'Thi': [78, 82, 202, 206, 276], 'Kim': [79, 83, 203, 207, 277], 'Loan': [80, 84, 204, 208, 278], 'Nguyen': [81, 85, 205, 209], 'Tina': [101, 103, 225, 227, 280], 'Jaenicke': [102, 104, 226, 228], 'Regine': [120, 124, 244, 248, 283], 'Hengge': [121, 125, 245, 249, 284], 'Corresponding': [122, 246], 'Author': [123, 247, 268], '[email': [126, 250, 301], 'protected]': [127, 251, 302], 'orcid.org/0000-0002-3969-8018': [128, 252], 'Information': [269], 'Herbst1,': [271], 'Lorkowski1,': [273], 'Sarenko1,': [275], 'Nguyen1,': [279], 'Jaenicke1': [281], '*,1': [285], '1Institut': [286], '*Corresponding': [294], 'author.': [295], 'Tel:': [296], '+49-30-2093-49686;': [297], 'Fax:': [298], '+49-30-2093-49681;': [299], 'E-mail:': [300], 'The': [303, 346, 524, 546, 566, 596, 718, 1059, 1198, 1430, 1532, 1775, 1948, 2505, 3209, 3468, 3536, 3605, 3648, 3704, 3877, 3932, 4075, 4349], 'EMBO': [304], 'Journal': [305], '(2018)37:e97825https://doi.org/10.15252/embj.201797825': [306], 'PDFDownload': [307], 'PDF': [308], 'article': [310], 'text': [311], 'main': [313], 'figures.': [314], 'Peer': [315], 'ReviewDownload': [316], 'summary': [318], 'the': [320, 418, 435, 443, 452, 499, 515, 563, 577, 612, 626, 639, 661, 711, 723, 787, 818, 886, 894, 916, 932, 942, 964, 981, 993, 1000, 1019, 1041, 1066, 1079, 1098, 1161, 1165, 1172, 1220, 1228, 1253, 1260, 1272, 1295, 1312, 1327, 1332, 1339, 1351, 1355, 1359, 1363, 1368, 1372, 1377, 1384, 1388, 1396, 1409, 1434, 1444, 1454, 1459, 1469, 1476, 1483, 1506, 1510, 1521, 1526, 1541, 1550, 1675, 1687, 1727, 1756, 1788, 1806, 1822, 1829, 1841, 1866, 1877, 1886, 1893, 1898, 1929, 1974, 1997, 2015, 2052, 2078, 2087, 2125, 2138, 2153, 2157, 2168, 2175, 2179, 2197, 2235, 2247, 2250, 2279, 2312, 2320, 2347, 2364, 2369, 2381, 2398, 2407, 2411, 2419, 2426, 2445, 2463, 2475, 2481, 2496, 2517, 2526, 2532, 2551, 2557, 2577, 2583, 2621, 2634, 2654, 2658, 2670, 2692, 2720, 2730, 2754, 2761, 2771, 2787, 2791, 2797, 2864, 2871, 2874, 2904, 2912, 2922, 2937, 2967, 2986, 2992, 3009, 3012, 3019, 3034, 3071, 3078, 3093, 3098, 3101, 3116, 3121, 3131, 3139, 3148, 3151, 3154, 3163, 3168, 3171, 3203, 3236, 3241, 3244, 3279, 3301, 3304, 3317, 3326, 3333, 3339, 3382, 3391, 3405, 3414, 3439, 3452, 3507, 3543, 3546, 3555, 3562, 3575, 3612, 3619, 3628, 3631, 3634, 3643, 3653, 3665, 3684, 3687, 3691, 3712, 3715, 3731, 3734, 3739, 3743, 3760, 3767, 3808, 3828, 3847, 3864, 3873, 3940, 3951, 3960, 3981, 3988, 4043, 4055, 4059, 4062, 4086, 4100, 4114, 4140, 4156, 4167, 4193, 4199, 4220, 4236, 4254, 4275, 4306, 4325, 4340, 4389, 4403, 4413, 4423, 4436, 4450, 4454], 'editorial': [321, 325], 'decision': [322, 326], 'including': [324], 'letters,': [327], 'reviewer': [328], 'comments': [329], 'responses': [332], 'to': [333, 336, 931, 944, 1064, 1186, 1202, 1219, 1362, 1394, 1439, 1502, 1712, 1717, 1754, 1983, 1999, 2148, 2254, 2260, 2346, 2353, 2368, 2406, 2500, 2525, 2587, 2594, 2613, 2627, 2682, 2735, 2847, 2863, 2997, 3039, 3106, 3180, 3188, 3300, 3314, 3541, 3574, 3617, 3698, 3728, 3772, 3827, 4042, 4264, 4280, 4401], 'feedback.': [334], 'ToolsAdd': [335], 'favoritesDownload': [337], 'CitationsTrack': [338], 'CitationsPermissions': [339], 'ShareFacebookTwitterLinked': [340], 'InMendeleyWechatReddit': [341], 'Figures': [342], '&': [343, 680, 808, 873, 902, 954, 1224, 1264, 1855], 'Info': [344], 'Abstract': [345], 'nucleotide': [347], 'second': [348, 493, 654, 1484, 3705, 4076, 4221], 'messenger': [349, 527, 655], 'nearly': [351, 651], 'ubiquitously': [352], 'promotes': [353, 660, 2338, 3103, 4090], 'bacterial': [354, 525, 653, 4148], 'biofilm': [355, 530, 943, 2392], 'formation,': [356], 'with': [357, 389, 411, 451, 607, 735, 744, 803, 844, 1179, 1215, 1256, 1490, 1621, 1674, 1787, 1973, 2009, 2045, 2054, 2196, 2210, 2432, 2556, 2603, 2615, 2650, 2748, 2854, 2985, 3217, 3749, 3784, 3800, 3813, 3820, 3945, 4128, 4210, 4274, 4358], 'enzymes': [358, 759], 'that': [359, 383, 394, 429, 476, 656, 834, 925, 934, 940, 1126, 1184, 1233, 1239, 1453, 1487, 1577, 1592, 1625, 1646, 1662, 1741, 1870, 2390, 2514, 2896, 2991, 3044, 3051, 3075, 3278, 3296, 3312, 3329, 3342, 3419, 3571, 3663, 3676, 3872, 3884, 3977, 4019, 4048, 4152, 4323, 4372, 4412], 'synthesize': [360, 3998], 'degrade': [362], 'being': [364, 1217, 4363], 'controlled': [365, 540, 1560], 'diverse': [367], 'N-terminal': [368, 763, 1103], 'sensor': [369, 764, 805, 1104], 'domains.': [370], 'Here,': [371, 2038], 'we': [372, 427, 1451, 1765, 2917, 3042], 'describe': [373], 'class': [376], 'widely': [378, 1424], 'occurring': [379, 1425], 'phosphodiesterases': [381, 552, 633, 742], '(PDE)': [382], 'feature': [384, 762, 3903], 'periplasmic': [386, 463, 564, 578, 593, 1119, 1155, 1173, 1205, 1235, 1275, 1333, 1477, 1527, 2154, 2365, 2399, 2476, 2503, 2518, 2606, 2622, 2637, 2671, 2905, 2923, 3066, 3122, 3164, 3351, 3469, 3735, 3768, 3789, 4326], '"CSS': [387, 1156], 'domain"': [388], 'two': [390, 398, 850, 913, 982, 1111, 1128, 1878, 2072, 2617, 3191, 3422, 3947, 4341], 'highly': [391, 449, 470, 1129, 3062, 4356], 'conserved': [392, 574, 1130, 1328, 1431, 1522, 1879, 1954, 2412], 'cysteines': [393, 575, 1432, 1880, 1955], 'is': [395, 447, 460, 477, 556, 605, 649, 722, 784, 885, 963, 1035, 1136, 1175, 1269, 1350, 1462, 1488, 1500, 1558, 1797, 1802, 1814, 2909, 3047, 3061, 3069, 3090, 3162, 3175, 3297, 3308, 3330, 3483, 3578, 3624, 3637, 3669, 3695, 3709, 3854, 3860, 3886, 3972, 4003, 4050, 4080, 4153, 4329, 4440], 'flanked': [396], 'transmembrane': [399, 1112, 1344, 1485, 2361, 3706, 3740, 4077, 4222, 4342], 'regions': [400, 3741, 3949, 4343], '(TM1': [401, 1114], 'TM2)': [403, 1116], 'followed': [405], 'cytoplasmic': [408, 751, 1166, 2370, 4455], 'EAL': [409, 474, 745, 831, 1168, 1364, 1899, 1909, 1998, 2371, 2541, 3117, 3213, 3644, 3716, 3782, 3798, 3809, 3859, 3933, 3954, 4030, 4045, 4064, 4087, 4353, 4391, 4424, 4451], 'domain': [410, 421, 437, 495, 503, 580, 632, 832, 1335, 1365, 1390, 1412, 1461, 1479, 1529, 1692, 1763, 1910, 2159, 2322, 2367, 2401, 2477, 2676, 3014, 3068, 3156, 3636, 3645, 3717, 3725, 3737, 3770, 3810, 3934, 4046, 4065, 4088, 4328, 4392, 4425, 4452], 'PDE': [412, 439, 1906, 1987, 2063, 2118, 2327, 3050, 3182, 3488, 3567, 3583, 3640, 4083, 4333, 4347, 4397, 4431], 'activity.': [413, 440, 1448, 2064, 2340, 4348, 4432], 'Using': [414], 'one': [416, 785, 1133, 1407, 1873, 1951, 2333, 2469], 'five': [419, 1077, 1410, 1688], 'CSS': [420, 436, 494, 502, 549, 579, 613, 631, 1141, 1334, 1389, 1411, 1460, 1478, 1691, 1762, 1887, 2158, 2321, 2366, 2400, 2675, 3013, 3067, 3155, 3635, 3692, 3736, 3769, 4327], 'PDEs': [422, 504, 802, 856, 1045, 1081, 1413, 1693], 'Escherichia': [424, 643, 821, 2162, 4179], 'coli': [425, 521, 644, 822, 867, 884, 1048, 1084, 1416, 1574, 2163, 4180], 'K-12,': [426, 1417], 'show': [428, 1715, 3542], 'DsbA/DsbB-promoted': [430], 'disulfide': [431, 570, 1149, 2150, 2667], 'bond': [432, 571, 584, 1150, 2668], 'formation': [433, 531, 572, 585, 662, 1152, 2632, 2898, 3055, 3089, 4404], 'in': [434, 514, 562, 576, 611, 638, 657, 672, 696, 860, 882, 936, 1046, 1082, 1153, 1207, 1242, 1331, 1337, 1358, 1443, 1475, 1496, 1509, 1525, 1549, 1599, 1669, 1684, 1805, 1840, 1849, 2036, 2097, 2152, 2240, 2267, 2283, 2324, 2387, 2404, 2452, 2474, 2516, 2545, 2571, 2669, 2678, 2697, 2790, 2806, 2870, 2887, 2903, 2939, 2943, 2955, 2960, 2975, 3002, 3011, 3056, 3070, 3077, 3127, 3147, 3153, 3344, 3377, 3386, 3413, 3430, 3438, 3501, 3516, 3611, 3633, 3683, 3690, 3742, 3759, 3922, 3959, 4031, 4039, 4069, 4119, 4174, 4182, 4214, 4235, 4386], 'reduces': [438], 'By': [441, 1031], 'contrast,': [442, 1032, 4388], 'free': [444, 597, 1455, 3072, 3140, 4044, 4063], 'thiol': [445, 598, 1189, 1456, 3073, 3141], 'form': [446, 459, 599, 1457, 2149, 2354, 2501, 2588, 3040, 3142, 3667], 'enzymatically': [448], 'active,': [450, 4357], 'TM2': [453, 472, 2529, 2539, 3114, 3211, 3328, 3708, 3780, 3796, 3857, 3952, 3968, 3978, 4024, 4028, 4049, 4216, 4351, 4416, 4439], 'region': [454, 1108, 1486, 3264, 3295, 4223], 'promoting': [455], 'dimerization.': [456], 'Moreover,': [457, 795, 2373, 2600], 'processed': [461, 3398, 3409, 3548], 'proteases': [464, 594, 1298, 3123, 3423, 3470], 'DegP': [465, 3124, 3460, 3471], 'DegQ,': [467], 'yielding': [468], 'active': [471, 1498, 1894, 3063, 3670, 4321], '+': [473, 2540, 3212, 3779, 3781, 3797, 3858, 3953, 3969, 4029, 4352], 'fragment': [475, 2489, 2509, 2542, 2553, 2597, 2686, 2764, 2875, 2880, 2969, 3214, 3246, 3399, 3410, 3454, 3549, 3577, 3866, 4354], 'slowly': [478, 4026, 4051], 'removed': [479], 'further': [481, 1970, 2068, 3729, 4016], 'proteolysis.': [482, 1467], 'Similar': [483], 'was': [488, 623, 1062, 1778, 2042, 2457, 2554, 2733, 2744, 2778, 2861, 2884, 2935, 3230, 3411, 3435, 3539, 3936, 4015, 4203, 4217, 4251, 4310, 4318, 4355, 4383], 'also': [489, 1839, 1995, 2359, 2449, 2485, 2852, 2885, 3176, 3457, 3664, 3882, 4034, 4419], 'observed': [490, 624, 2626, 3412, 3878], 'PDE,': [496, 1764], 'PdeB.': [497, 629], 'At': [498], 'physiological': [500, 1071], 'level,': [501], 'modulate': [505], 'production': [506, 887, 962, 1667, 1748, 1945, 3450], 'supracellular': [508, 923], 'architecture': [509, 924, 3783], 'extracellular': [511, 533, 635, 889, 917, 1507], 'matrix': [512, 534, 636, 676, 918, 961, 1056, 1508, 1551, 1676, 1747, 1975, 2079, 2198, 2393, 3054, 4382], 'polymers': [513], 'deeper': [516, 640, 1511], 'layers': [517], 'mature': [519], 'E.': [520, 866, 883, 1047, 1083, 1287, 1415, 1517, 1573, 2388], 'biofilms.': [522, 646], 'Synopsis': [523], 'secondary': [526], 'controls': [529, 1835], 'component': [535], 'secretion.': [536], 'C-di-GMP': [537, 648], 'levels': [538, 1624, 2076, 2273, 2949], 'are': [539, 666, 703, 750, 1027, 1240, 1294, 1326, 1437, 1848, 2295, 2829, 3347, 3424, 3474, 3589, 4287, 4428], 'degradation': [542, 739, 2375, 2494, 4008], 'via': [543, 2633, 4105], 'phosphodiesterases.': [545], 'activity': [547, 588, 603, 1163, 1341, 1397, 1492, 1759, 1907, 2143, 2328, 3137, 3183, 3489, 3568, 3584, 3641, 3713, 4084, 4304, 4398], 'domain-containing': [550], 'PdeC': [553, 587, 601, 1088, 1447, 1531, 1744, 1767, 1931, 2145, 2170, 2339, 2377, 2403, 2428, 2466, 2497, 2584, 2601, 2681, 2712, 2820, 2908, 2931, 3036, 3046, 3060, 3128, 3144, 3197, 3491, 3529, 3681, 3700, 3719, 3747, 3840, 3891, 3915, 4093, 4102, 4116, 4142, 4212, 4308, 4427], 'PdeB': [555], 'regulated': [557], 'complex': [559, 568, 978], 'posttranslational': [560], 'modifications': [561], 'space.': [565], 'DsbA/DsbB': [567, 1199, 1435, 2420, 2913, 3094], 'induces': [569], 'between': [573, 618, 729, 2899, 3133, 3791], 'PdeC.': [582, 3551, 3647], 'Disulfide': [583], 'inhibits': [586], 'proteolytic': [590, 1473, 2493, 2763, 3027, 3104, 3177, 3237, 3245, 3477, 3622, 3678, 3865, 3942, 4007, 4239], 'cleavage': [591, 610, 3105, 3146, 3178, 3608, 3905], 'DegP/DegQ.': [595], 'shows': [602], 'which': [604, 665, 997, 1135, 1158, 1279, 1300, 1495, 1557, 1833, 1904, 2572, 2867, 3111, 3239, 3765, 3870, 3995, 4165, 4215, 4445], 'associated': [606, 1489, 2555], 'dimerisation': [608], 'domain.': [614, 1105, 1169, 2372, 3118], 'A': [615, 876, 957, 1619, 3253], 'similar': [616, 4207], 'interplay': [617], 'related': [627], 'Active': [630], 'reduce': [634, 1503], 'biosynthesis': [637, 1504], 'zones': [641, 1512], 'macrocolony': [645, 910, 1514, 1697, 1937, 1967, 2040, 3057, 3650, 4313], 'Introduction': [647], 'ubiquitous': [652], 'many': [658, 761, 1204], 'species': [659], 'biofilms': [664, 702, 1515], 'multicellular': [667], 'aggregates': [668], 'bacteria': [670, 700, 797], 'encased': [671], 'self-produced': [674], 'protective': [675], 'biopolymers': [678], '(Jenal': [679, 807], 'Malone,': [681, 809], '2006;': [682, 810, 904], 'Römling': [683, 813], 'et': [684, 688, 715, 814, 869, 898, 906, 969, 973, 1005, 1009, 1195, 1320, 1564, 1568, 1721, 1859, 1913, 1917, 1921, 3354, 3358, 3362, 3832, 4162], 'al,': [685, 689, 716, 815, 870, 899, 907, 970, 974, 1006, 1010, 1196, 1321, 1565, 1569, 1722, 1860, 1914, 1918, 1922, 3355, 3359, 3363, 3833, 4163], '2013;': [686, 1011, 1566], 'Jenal': [687], '2017).': [690], 'Biofilms': [691], 'play': [692, 1440], 'an': [693, 1176, 1180, 1381, 1537, 1572, 1957, 2736, 3803, 3816, 3887, 4243, 4320], 'important': [694], 'role': [695, 2417], 'chronic': [697], 'infections': [698], 'since': [699, 1989, 2120], 'within': [701], 'tolerant': [705], 'against': [706], 'antibiotics': [707], 'better': [709], 'evade': [710], 'immune': [712], 'system': [713, 1200, 1262, 1436, 1771, 2421, 2926, 2994, 4151], '(Hall-Stoodley': [714], '2004).': [717], 'cellular': [719, 1985, 2075, 2117, 2948], 'level': [721, 4378], 'result': [724, 1668], 'dynamic': [727], 'balance': [728], 'synthesis': [730], 'diguanylate': [732], 'cyclases': [733], '(DGC,': [734], 'GGDEF': [736, 829], 'domains)': [737], 'specific': [741, 1038, 2285, 3286], '(PDE,': [743], 'or': [746, 1281, 1306, 1681, 1826, 1874, 1883, 1891, 1952, 2012, 2191, 2334, 2470, 2610, 2620, 2802, 3174, 3199, 3498, 3512, 3525, 3626], 'HD-GYP': [747], 'domains).': [748], 'There': [749], 'as': [752, 754, 769, 771, 1191, 1303, 1307, 1419, 1639, 1731, 1768, 1962, 2035, 2282, 2459, 2543, 2657, 3365, 3367, 3671, 3701, 3722, 3758, 3843, 4118, 4171], 'well': [753, 770, 1029, 3366], 'membrane-attached': [755], 'variants': [756, 1949, 2053, 2146, 2429, 2467, 2498, 2585, 2602, 2713, 2821, 3037, 3530, 4103, 4117, 4143, 4309], 'these': [758, 792, 912, 1343, 1399, 1713, 1750, 2350, 2646, 3190, 3421, 3837, 3999, 4010], 'domains—including': [765], 'PAS,': [766], 'GAF,': [767], 'REC': [768], 'various': [772, 861], 'membrane-integrated': [773], 'domains—that': [774], 'their': [776, 1208, 2437, 4106], 'enzymatic': [777, 1162, 1340, 1491, 3136], 'activities.': [778], 'In': [779, 848, 909, 1014, 1170, 1286, 1449, 1752, 1811, 2549, 2566, 2642, 2845, 2915, 3186, 3444, 3726], 'general,': [780, 1171], 'however,': [781, 3449], 'sensory': [782], 'input': [783], 'least': [788, 1743], 'understood': [789], 'aspects': [790], 'c-di-GMP-related': [793], 'enzymes.': [794], 'most': [796], 'possess': [798, 1097], 'multiple': [799, 1682], 'DGCs': [800, 852, 1043], 'different': [804, 2427, 2792, 3035], 'domains': [806], 'Hengge,': [811, 874, 955, 1012, 1856], '2009;': [812, 3360], '2013).': [816, 1266, 1923], 'Thus,': [817, 1571, 2311, 3852], 'Gram-negative': [819], 'bacterium': [820], 'K-12': [823, 2164], 'has': [824, 3776], 'complement': [826], '29': [828], 'and/or': [830, 3307], 'proteins': [833, 843, 1206, 1236, 1911, 2446, 2655, 4000, 4011], 'include': [835], '12': [836], 'DGCs,': [837], '13': [838, 1080], 'PDEs,': [839], 'four': [841, 854], '"degenerate"': [842], 'alternative': [845], 'non-enzymatic': [846], 'functions.': [847], 'addition,': [849, 1450, 1812, 2550, 2916, 3899], 'have': [857, 1380], 'been': [858], 'detected': [859, 2724, 2886], 'other': [862, 1042, 1229], 'commensal': [863], 'pathogenic': [865], '(Hengge': [868], '2015;': [871], 'Povolotsky': [872], '2016).': [875, 1013], 'major': [877, 1289, 2382, 3350], 'target': [878, 2383], 'signaling': [881, 2362], 'amyloid': [890], 'curli': [891, 1545, 1594, 1632, 1664, 1941, 2088, 2139], 'fibers': [892, 1546, 1595, 1633], 'exopolysaccharide': [895], 'cellulose': [896, 1548, 1635, 1666, 1944], '(Zogaj': [897], '2001;': [900, 971], 'Barnhart': [901], 'Chapman,': [903], 'Hufnagel': [905], '2015).': [908, 1861], 'biofilms,': [911], 'components': [914, 1580], 'assemble': [919], 'into': [920, 949, 1054, 1651, 1701, 1865, 1889, 1926, 3262, 3987], 'distinct': [922], 'confers': [926], 'stability,': [927], 'cohesion,': [928], 'elasticity': [930], 'biofilm,': [933, 1556], 'is,': [935, 2391, 3076, 3420, 3572, 4324, 4373], 'essence': [937], 'tissue-like': [938], 'properties': [939], 'allow': [941], 'fold': [945], 'buckle': [947, 1649], 'up': [948, 1650], 'macroscopic': [950], 'morphological': [951, 2211], 'structures': [952], '(Serra': [953, 1854], '2014).': [956], 'key': [958], 'activator': [959], 'transcription': [965, 994], 'factor': [966, 995, 1824], 'CsgD': [967, 1003, 2081, 2108, 2136, 2272], '(Prigent-Combaret': [968], 'Brombacher': [972], '2003).': [975], 'Via': [976], 'cascade': [979], 'mechanism,': [980, 1018], 'PDE/DGC': [983], 'pairs': [984], 'PdeH/DgcE': [985], '(formerly': [986], 'YhjH/YegE)': [987], 'PdeR/DgcM': [989], '(YciR/YdaM)': [990], 'can': [991, 1249, 1301, 1745, 2378, 3052, 3985], 'activate': [992, 3699], 'MlrA,': [996], 'then': [998, 4434], 'drives': [999], 'expression': [1001, 1719, 1801, 1838, 2085, 2134, 2575], '(Pesavento': [1004], '2008;': [1007], 'Lindenberg': [1008, 1920], 'genetic': [1016], 'switch': [1017], 'functions': [1020, 1039], 'DgcE,': [1022], 'DcgM,': [1023], 'PdeH,': [1024], 'PdeR': [1026], 'now': [1028], 'defined.': [1030], 'much': [1033], 'less': [1034, 1940, 2043], 'known': [1036], 'about': [1037, 1122, 3231], 'whether': [1050, 2007, 2849, 2919, 3374, 3621, 3627, 4438], 'they': [1051, 1990, 2121], 'somehow': [1052], 'feed': [1053], 'mechanism.': [1058], 'present': [1060], 'study': [1061, 1755], 'undertaken': [1063], 'characterize': [1065], 'mode': [1067, 1853], 'activation': [1069, 3744], 'function': [1072, 3022, 3337], 'group': [1075], 'K-12—PdeB': [1085], '(previously': [1086], 'YlaB),': [1087], '(YjcC),': [1089], 'PdeD': [1090], '(YoaD),': [1091], 'PdeG': [1092], '(YcgG),': [1093], 'PdeN': [1095], '(Rtn)—that': [1096], 'same': [1099, 2280, 4115], 'putative': [1102], 'This': [1106, 1146, 1267, 2341, 2357, 2934, 2989, 3276, 4410], 'protein': [1107, 2453, 3824, 3844, 3919, 4202], 'consists': [1109, 3112], 'segments': [1113], 'flanking': [1117], 'loop': [1120, 1528, 2155, 2519, 2672, 2906, 3790], '200': [1123], 'amino': [1124, 3248, 3257, 3287], 'acids': [1125, 2031], 'contains': [1127, 3875], 'cysteine': [1131, 1329, 1356, 1523, 2056, 2351, 2413, 2472, 2607, 2618, 2638], 'residues,': [1132], 'part': [1137, 3889, 4022, 4196], 'characteristic': [1140], 'motif': [1142, 1888, 1900], '(Appendix': [1143, 1616, 1705, 1737, 1772, 2563, 2639, 3273, 3463, 3910, 3963], 'Fig': [1144, 1617, 1706, 1738, 1773, 2220, 2546, 2564, 2640, 3225, 3228, 3274, 3464, 3911, 3930, 3964, 4229], 'S1).': [1145], 'suggested': [1147, 2342, 3277], 'potential': [1148, 2348], '(DSB)': [1151], 'domain",': [1157], 'might': [1159], 'C-terminal': [1167, 2438, 2685, 2715, 3755], 'space': [1174], 'oxidizing': [1177], 'environment': [1178], 'intricate': [1181], 'homeostasis': [1183, 1268], 'allows': [1185, 4166], 'use': [1187], 'DSB/free': [1188], 'transitions': [1190], 'switches': [1193], '(Wouters': [1194], '2010).': [1197], 'helps': [1201], 'stabilize': [1203], 'native': [1209], 'functional': [1210], 'conformations': [1211], 'introducing': [1213, 1996], 'DSBs,': [1214, 3041], 'electrons': [1216, 1257], 'delivered': [1218], 'respiratory': [1221], 'chain': [1222, 3319], '(Ito': [1223], 'Inaba,': [1225], '2008).': [1226], 'On': [1227, 3835], 'hand,': [1230], 'wrong': [1231], 'DSBs': [1232], 'render': [1234], 'non-functional': [1237], 'generated': [1241, 2431, 2478, 2520, 3867], 'particular': [1243], 'under': [1244, 1749, 2172, 2856], 'conditions': [1245], 'oxidative': [1247], 'stress': [1248], 'be': [1250, 3207, 4265], 'resolved': [1251], 'DsbD/DsbC/DsbG': [1254, 2925, 2993], 'system,': [1255], 'provided': [1258], 'thioredoxin': [1261], '(Cho': [1263], 'Collet,': [1265], 'complemented': [1270], 'presence': [1273, 2313, 4414], 'chaperones': [1276], 'proteases,': [1278], 'refold': [1280], 'remove': [1282], 'misfolded': [1283], 'proteins,': [1284], 'respectively.': [1285], 'coli,': [1288, 2389], 'factors': [1290], 'kind': [1293], 'HtrA': [1296], 'family': [1297], 'DegP,': [1299, 3348], 'act': [1302], 'chaperone': [1305], 'protease': [1309, 3280, 3311], 'depending': [1310], 'on': [1311, 1383, 2026, 2116, 2187, 2930, 3459, 4122, 4155, 4185, 4269, 4360], 'actual': [1313], 'conditions,': [1314, 2859], 'its': [1316, 1497, 1793, 2616, 2674, 3065, 3270, 3368, 3946], 'homolog': [1317, 3369], 'DegQ': [1318, 3126, 3462, 3473], '(Clausen': [1319], '2011).': [1322], 'Given': [1323], 'context,': [1325], 'residues': [1330, 1357, 1524, 2352, 2414, 2473, 2619], 'involved': [1336, 3281], 'controlling': [1338], 'PDEs?': [1345, 1400], 'And': [1346], 'if': [1347], 'so,': [1348], 'how': [1349], 'state': [1353, 1386, 1471, 1499, 2409], 'periplasm': [1360], 'signaled': [1361], 'located': [1366], 'at': [1367, 1742, 2206, 2436, 2753, 3320, 3926, 4054, 4135, 4453], 'inner': [1369, 4056], 'side': [1370, 4057, 4456], 'membrane?': [1373], 'Does': [1374], 'any': [1375, 1685, 2049, 2928], 'Dsb': [1378], 'systems': [1379], 'impact': [1382], 'thereby': [1392], 'contribute': [1393, 2996], 'These': [1401, 1979, 2019, 2065, 2112], 'questions': [1402], 'were': [1403, 1863, 1981, 1991, 2003, 2021, 2067, 2122, 2185, 2238, 2244, 2274, 2291, 2430, 2591, 2648, 2695, 2702, 2723, 2804, 2810, 2823, 2952, 3260, 3514, 3519, 3532, 3752, 3956, 4144, 4267], 'addressed': [1404, 4435], 'here': [1405], 'using': [1406, 2090, 4146, 4312], 'representative': [1421], 'PDE.': [1429, 3673, 3703], 'shown': [1438, 1501, 2266, 2544, 4277], 'crucial': [1441, 4429], 'roles': [1442, 3732], 'demonstrate': [1452], 'substrate': [1464], 'DegP/DegQ-mediated': [1466], 'Furthermore,': [1468, 2878], 'processing': [1474, 2396, 2679, 3028, 3129, 3271, 3427, 3478, 3482, 3573, 3623, 3679, 4240], 'dimerization': [1481, 3724, 4091, 4234], 'coli.': [1518], 'Results': [1519], 'Eliminating': [1520], 'activates': [1530], 'morphology': [1533, 2041, 2131, 3553, 4314], 'macrocolonies': [1535, 1736, 1845, 2025, 2214, 4359, 4407], 'provides': [1536], 'excellent': [1538], 'indicator': [1539], 'relative': [1542], 'amount': [1543], 'exquisitely': [1559], '(Lindenberg': [1563], 'Serra': [1567, 1858], '2013a,b).': [1570], 'strain': [1575, 1591, 1620, 1927, 2010, 2165, 2224, 2689, 2774, 2784, 3494, 4097, 4111, 4177, 4250], '(AR3110)': [1576], 'produces': [1578, 1626], 'both': [1579, 1631, 1875, 1953, 2315, 2335, 2471], 'generates': [1581, 1610], 'spatially': [1583], 'ordered': [1584], 'pattern': [1585, 1603, 3272], 'ridges': [1587], 'wrinkles,': [1589], 'makes': [1593], 'only': [1596, 1647, 2480, 2608, 3785, 4365], '(W3110)': [1597], 'grows': [1598, 1638], 'concentric': [1601], 'ring': [1602], 'cellulose-only': [1606], 'producer': [1607], '(AR3110': [1608, 1636], 'csgBA)': [1609], 'multitude': [1612], 'tiny': [1614], 'wrinkles': [1615], 'S2A).': [1618], 'elevated': [1622], 'very': [1627, 1654, 3787, 4395], 'high': [1628, 1655, 3566, 3582], 'amounts': [1629], 'pdeH)': [1637], 'flat,': [1640], 'large,': [1641], 'rather': [1643, 3291, 3892], 'stiff': [1644], 'colonies': [1645, 1671, 4263], 'occasionally': [1648, 3007], 'long': [1652], 'ridges,': [1656], 'while': [1657, 2104, 2973, 3677], 'regulatory': [1658], 'mutations': [1659, 1683, 2789, 2974, 3376, 3393, 3500], '(rpoS,': [1660], 'csgD)': [1661], 'eliminate': [1663], 'unstructured': [1670, 2059, 4366], 'not': [1672, 2479, 2936, 2971, 2981, 3283, 3436, 3484, 3579, 3696, 3902, 4330, 4345, 4364, 4374, 4399], 'stained': [1673], 'dye': [1677, 1976, 2199], 'Congo': [1678, 1977, 2200, 4129, 4380], 'red.': [1679, 1978], 'Single': [1680], 'genes': [1689, 1714, 2794], 'encoding': [1690], 'did': [1694, 2970, 2980, 3914], 'hardly': [1695, 2048], 'affect': [1696, 1746, 2380], 'morphology,': [1698, 1938], 'when': [1699, 3064, 3920], 'introduced': [1700, 1864, 1925, 3261, 3822], 'AR3110': [1703, 2011, 2166, 3560, 4112], 'background': [1704, 2958], 'S2B),': [1707, 1739], 'even': [1708, 1958, 4375], 'though': [1709], 'reporter': [1710, 2093, 2231], 'fusions': [1711], 'low': [1716, 1782, 2180, 4396], 'moderate': [1718, 1800], '(Sommerfeldt': [1720], '2009).': [1723], 'Only': [1724], 'mutants': [1725, 2965], 'carrying': [1726, 2226, 2786, 3496, 3561], 'pdeC': [1728, 1776, 1836, 1867, 2017], 'deletion': [1729], 'grew': [1730], 'slightly': [1732, 2533, 3861], 'larger': [1733, 3918], 'flatter': [1735], 'suggesting': [1740, 2492, 4047], 'conditions.': [1751], 'order': [1753, 2846, 3187, 3727], 'chose': [1766], 'model': [1770], 'S3A).': [1774], 'gene': [1777], 'cloned': [1779, 2538, 3210, 4350], 'onto': [1780], 'copy': [1783, 2181, 2229], 'number': [1784, 2182], 'plasmid': [1785, 2183, 2732, 2773], '(pCAB18),': [1786], 'tac': [1789, 2176], 'promoter': [1790, 1817, 2177], '(ptac)': [1791], 'driving': [1792], 'expression.': [1794, 2111], 'Since': [1795], 'ptac': [1796, 1813, 2579, 4002], 'relatively': [1798], 'leaky,': [1799], 'conveniently': [1803], 'obtained': [1804, 2769], 'absence': [1807, 3079, 3099, 3149, 3169, 3302, 3606, 3629, 3685, 3982, 4237], 'inducer': [1809], '(IPTG).': [1810], 'vegetative': [1816], '(activated': [1818], 'RNAP': [1820], 'containing': [1821, 2234, 2691, 2729, 2796, 3506], 'sigma': [1823], 'RpoD': [1825], 'σ70),': [1827], 'which—unlike': [1828], 'stationary': [1830, 2568, 2643, 2944, 3003, 3992, 4070], 'phase': [1831, 2569, 2644, 2945, 3004, 3993, 4071], 'σS,': [1832], 'naturally': [1834], 'expression—drives': [1837], 'lower': [1842, 2133, 2326, 2954], 'layer': [1843], 'where': [1846, 2746], 'cells': [1847, 2946, 3005], 'post-exponential': [1851, 2705, 2813, 3522], 'growth': [1852, 2248, 2257, 2706, 2777, 2814, 3523], '2014;': [1857], 'Mutations': [1862], 'coding': [1868], 'sequence': [1869, 3897], 'eliminated': [1871, 2057, 2107], 'either': [1872, 2055, 2800, 3194, 3510], '(C75A;': [1881], 'C106A': [1882], '"ASS",': [1884], 'changing': [1885], 'ASS)': [1890], 'replaced': [1892, 4218], 'site': [1895, 3906], 'glutamate': [1896], 'alanine': [1902], '("AAL"),': [1903], 'eliminates': [1905], '(Schmidt': [1912], '2005;': [1915], 'Tchigvintsev': [1916], '2010;': [1919], 'When': [1924], 'AR3110,': [1928], 'wild-type': [1930, 2957], '(PdeCwt)': [1932], 'construct': [1933], 'clearly': [1934, 2379, 3661, 4319], 'reduced': [1935, 1971], 'wrinkly': [1936], 'indicating': [1939, 2895, 4018], 'fiber': [1942], '(Fig': [1946, 2082, 2095, 2490, 2598, 2876, 2893, 2932, 3023, 3029, 3388, 3659, 3850, 4012, 4315, 4408], '1A).': [1947], 'lacking': [1950, 2014, 2468], 'had': [1956, 2927], 'stronger': [1959], 'effect,': [1960, 2050], 'visible': [1961], 'total': [1964], 'lack': [1965], 'structure': [1968, 3335], 'staining': [1972], 'effects': [1980, 2113, 3396], 'due': [1982], 'enhanced': [1984, 2062], 'activity,': [1988, 2119, 4334], 'completely': [1992, 2106, 2592, 4067], 'suppressed': [1993, 2123], 'AAL': [2000, 2126], 'mutation.': [2001, 2127], 'Effects': [2002], 'similar,': [2004], 'no': [2005, 2580, 3408, 3996], 'matter': [2006], 'tested': [2008, 2918, 3373, 4145, 4311], 'derivatives': [2013, 3748], 'chromosomal': [2016], 'gene.': [2018], 'findings': [2020], 'confirmed': [2022, 3883], 'growing': [2024, 2865, 2940], 'YESCA': [2027, 2192], 'medium': [2028], '(containing': [2029, 2714], 'casamino': [2030], 'instead': [2032], 'tryptone': [2034], 'LB).': [2037], 'overall': [2039], 'pronounced,': [2044], 'PdeCwt': [2046, 2102, 2456, 2614, 2731, 2801, 2855, 2883, 2951, 3001, 3032, 3434, 3456, 3511], 'having': [2047], 'whereas': [2051, 2331, 3938], 'showed': [2058, 3394, 4394], 'macrocolonies,': [2060], 'reflecting': [2061], 'results': [2066, 2323, 2677, 3020], 'corroborated': [2069], 'monitoring': [2071], 'c-di-GMP-dependent': [2073], 'functions:': [2074], 'regulator': [2080], '1B)': [2083], 'operon': [2089, 2140], 'csgB::lacZ': [2092, 2110, 2230, 2290], 'fusion': [2094, 2232], '1C)': [2096], 'liquid': [2098], 'medium.': [2099, 2242, 2808], 'Also': [2100], 'here,': [2101], 'reduced,': [2103], 'PdeCASS': [2105, 2772, 2803, 3385, 3429, 3513, 3610, 3658, 3668], 'equally': [2114], 'depended': [2115, 3458], 'Figure': [2128, 2304, 2662, 2838, 3466, 3598, 4073, 4296], '1.': [2129], 'Macrocolony': [2130, 3552], 'indicates': [2141, 2360, 3565], 'increased': [2142], 'unable': [2147, 2499, 2586, 3038], 'bonds': [2151], 'Macrocolonies': [2160, 4109], 'expressing': [2167, 2799, 3509, 3657, 4099, 4113], 'indicated': [2169, 2236, 2513, 2693, 2721, 2788, 3563, 3662, 4101, 4141], 'versions': [2171], 'from': [2178, 2289, 2363, 2576, 2708, 2719, 2770, 2816, 2882, 3325, 3455, 3868], 'pCAB18': [2184], 'grown': [2186, 2239, 2696, 2734, 2805, 3515, 4121, 4268], 'salt-free': [2188, 4123], 'LB': [2189, 2241, 2698, 2807, 4124], '(LBnoS)': [2190], 'agar': [2193, 4125, 4187, 4272], 'plates': [2194, 4126, 4362], 'supplemented': [2195, 4127], 'red': [2201, 4130, 4183], '(CR)': [2202, 4131], '5': [2204, 4133], 'days': [2205, 4134], '28°C.': [2207, 4136], 'For': [2208, 4205, 4259], 'comparison': [2209], 'patterns': [2212], 'standard': [2216], 'strains,': [2217], 'see': [2218, 3223, 4227], 'Appendix': [2219, 3224, 3929, 4228], 'S1.': [2221], 'Derivatives': [2222, 2687, 2782, 3492], 'W3110': [2225, 2690, 2728, 2785, 3495, 4098], 'single': [2228, 2605, 2635, 3256, 3392, 3497, 4271], 'plasmids': [2237, 2694, 2722, 2798, 3508, 3564], 'Samples': [2243, 2809, 3518], 'taken': [2245, 2703, 2752, 2811, 3520, 4278], 'during': [2246, 2704, 2812, 3521], 'cultures': [2251, 2818], '(numbers': [2252], 'refer': [2253], 'hours': [2255], 'respond': [2259], 'data': [2261, 2294, 2828, 3588, 4286], 'sampling': [2262], 'measurement': [2264], 'points': [2265], 'C;': [2268], 'on,': [2269], 'overnight).': [2270], 'Cellular': [2271], 'determined': [2275, 3331], 'immunoblotting.': [2277, 2726, 2826, 3535], 'From': [2278], 'samples': [2281, 2701, 2749], '(B),': [2284], 'β-galactosidase': [2286], 'activities': [2287], 'expressed': [2288, 2822, 3531, 3921], 'determined.': [2292], 'available': [2296, 2830, 3590, 4288], 'online': [2297, 2831, 3591, 4289], 'figure.': [2300, 2834, 3594, 4292], 'Data': [2302, 2836, 3596, 4257, 4294], '1': [2305], '[embj201797825-sup-0002-SDataFig1.jpg]': [2306], 'Download': [2307, 2309, 2841, 2843, 3601, 3603, 4299, 4301], 'figure': [2308, 2842, 3602, 4300], 'PowerPoint': [2310, 2844, 3604, 4302], 'Cys75': [2316, 2900, 3084], 'Cys106': [2318, 2902], 'eliminating': [2332], 'them': [2337], 'possible': [2344], 'link': [2345], 'DSB.': [2356, 2504], 'observation': [2358], 'regulation': [2386], 'production.': [2394], 'Proteolytic': [2395, 3145], 'response': [2405], 'To': [2422, 2440, 4231], 'expression,': [2425], 'His6': [2434, 2716, 3756, 4107], 'tag': [2435], 'ends.': [2439], 'our': [2441], 'surprise,': [2442], 'detection': [2443], 'immunoblotting': [2448, 2751], 'revealed': [2450, 4005], 'changes': [2451], 'size:': [2454], 'Whereas': [2455], 'visualized': [2458, 2824, 3533, 4104], 'band': [2461, 2483, 4037], 'expected': [2464, 3848], 'size,': [2465], 'full-size': [2482, 3406, 3666, 3761], 'but': [2484, 2942, 3267, 3290, 3481, 4367], 'prominent': [2487], 'shorter': [2488, 3108, 3862], '2A),': [2491], 'size': [2506, 2535, 3879, 4040], '(approximately': [2510], '33': [2511], 'kD)': [2512], 'new': [2522], 'N-terminus': [2523, 3242], 'close': [2524, 4336], 'beginning': [2527], '(confirmed': [2530], 'smaller': [2534, 2596, 2968, 3234], '4A': [2547], 'below).': [2548], 'PdeCASS-derived': [2552, 3941], 'membrane': [2558, 3961, 4060, 4420], 'fraction': [2559, 3962], 'cell': [2561], 'extracts': [2562], 'S4).': [2565, 3965], 'overnight': [2567, 2709, 2776, 2817, 3526, 3991], 'cultures,': [2570], 'de': [2573], 'novo': [2574], 'σ70-driven': [2578], 'longer': [2581, 3855, 3997], 'occurs,': [2582], 'DSB': [2590, 2631, 2897, 3010, 3082, 3088, 3102, 3152, 3172, 3632, 3689], 'degraded': [2593], '2A).': [2599], '(PdeCASS': [2609], 'PdeCC75A)—in': [2611], 'contrast': [2612], 'cysteine-free': [2623], 'variant': [2624], 'PdeCASS/C75A—were': [2625], 'dimerize': [2628], 'intermolecular': [2630, 3402], 'remaining': [2636], 'S5).': [2641], 'cells,': [2645, 2941, 3994], 'dimers': [2647, 3403], 'resolved,': [2649], 'essentially': [2651, 4369], 'all': [2652, 3957, 4262], 'accumulating': [2656], 'small': [2659], '33-kD': [2660, 2684, 2762, 3453, 3547, 3576], 'fragment.': [2661], '2.': [2663], 'Absence': [2664], 'medium,': [2699], '("on")': [2710], 'cultures.': [2711], 'tags)': [2717], 'produced': [2718, 4033], 'Strain': [2727], 'OD578': [2737], '1.2': [2739], '6.5': [2741], 'mM': [2742], 'DTT': [2743, 2860], 'added': [2745, 2862], 'indicated,': [2747], 'times': [2755], 'indicated.': [2756], 'As': [2757, 4189], 'reference': [2759], 'sample': [2768], 'after': [2775, 3219, 3802, 3815], 'included': [2779, 3540], '(last': [2780], 'lane).': [2781], 'dsb': [2793], '2': [2839, 3232], '[embj201797825-sup-0003-SDataFig2.jpg]': [2840], 'test': [2848], 'occurs': [2853, 3682], 'strongly': [2857], 'reducing': [2858], 'culture,': [2866], 'indeed': [2868], 'resulted': [2869], 'appearance': [2872, 3383], '2B).': [2877], 'derived': [2881], 'dsbA': [2888, 3446, 3537], 'dsbB': [2890], 'mutant': [2891, 3198, 3538, 3615, 3656, 3925], 'backgrounds': [2892], '2C),': [2894], 'promoted': [2910, 3091], 'system.': [2914], 'knocking': [2920], 'out': [2921], 'DSB-reducing': [2924], 'effect': [2929], '2C).': [2933], 'case': [2938], '(overnight),': [2947], 'reproducibly': [2953], 'than': [2959, 3235, 3863, 3893], 'dsbD,': [2961, 2976], 'dsbC,': [2962, 2977], 'dsbG': [2964, 2979], '(although': [2966], 'accumulate),': [2972], 'make': [2982], 'difference': [2984], 'DSB-free': [2987], 'PdeCASS.': [2988, 3252, 3586], 'suggests': [2990], 'may': [2995], 'slow': [2998], 'destabilization': [2999], 'attacking': [3008], 'PdeCwt.': [3016], 'Taking': [3017], 'together': [3018], 'concerning': [3021], '1)': [3024], 'redox-dependent': [3026], '2)': [3030], 'conclude': [3043, 3675], '(i)': [3045, 3763], 'c-di-GMP-degrading': [3049], 'down-regulate': [3053], 'biofilms;': [3058], '(ii)': [3059, 3795], 'state,': [3074], 'linking': [3083], 'Cys106;': [3086], '(iii)': [3087, 3807], 'system;': [3095], '(iv)': [3097], 'membrane-associated': [3109], 'polypeptide': [3110, 3294, 3318, 3955], 'Role': [3119], 'relationship': [3132], 'raised': [3157], 'interesting': [3159], 'questions.': [3160], 'What': [3161], 'protease(s)': [3165, 3205], 'involved?': [3166], 'Is': [3167], 'sufficient': [3173, 3638, 4400], 'required': [3179, 3580, 3625, 3697, 3710, 4081, 4331], 'stimulate': [3181], 'PdeC?': [3185], 'separate': [3189], 'processes': [3192], 'genetically,': [3193], 'non-cleavable': [3196], 'knockout': [3201, 3375, 3447], 'relevant': [3204], 'would': [3206], 'required.': [3208], '(which': [3215], 'begins': [3216], 'Ser231': [3218, 3801], 'starting': [3221], 'methionine;': [3222], 'S3': [3226], '4A)': [3229], 'kD': [3233], 'fragment,': [3238, 3943], 'placed': [3240], 'around': [3247], 'acid': [3249, 3258, 3288], '215': [3250], 'series': [3254], 'exchanges': [3259], 'PdeCASS,': [3266, 3869, 4242], 'none': [3268], 'altered': [3269], 'S6).': [3275], 'does': [3282, 3901, 4344], 'recognize': [3284], 'motif,': [3289], 'attacks': [3292], 'unfolded': [3298], '(due': [3299], 'stabilizing': [3305], 'DSB)': [3306], 'processive': [3310], 'stops': [3313], 'move': [3315], 'along': [3316], 'certain': [3322], 'minimal': [3323], 'distance': [3324], 'membrane-inserted': [3327], 'molecular': [3334], 'protease.': [3340], 'Proteases': [3341], 'operate': [3343], 'manner': [3346], 'chaperone/protease': [3352], '(Krojer': [3353], '2002;': [3356], 'Meltzer': [3357], 'Clausen': [3361], '2011),': [3364], 'DegQ.': [3370], 'We': [3371, 3674, 4433], 'therefore': [3372], 'degP': [3378, 3415, 3440, 3502, 3556, 3613, 3654, 4246], 'degQ': [3380, 3416, 3441, 3504, 3557, 3614, 3655, 4247], 'affected': [3381, 3437], 'immunoblots': [3387], '3A).': [3389], 'While': [3390], 'partial': [3395], '(less': [3397], 'protein),': [3407], 'double': [3417, 3442, 3499], 'mutant;': [3418], 'responsible': [3425, 3475], 'redundant': [3432], 'manner.': [3433], 'mutant.': [3443], 'background,': [3448], 'S7).': [3465], '3.': [3467], 'prerequisite': [3486], 'LB.': [3517], '(pe)': [3524], '(on),': [3527], 'position': [3544], 'derivative': [3558], 'PdeCASS;': [3570], '3': [3599], '[embj201797825-sup-0004-SDataFig3.jpg]': [3600], 'allowed': [3616], 'address': [3618], 'question': [3620, 4437], 'non-wrinkled': [3649], 'morphotype': [3651], '3B)': [3660], 'intramolecular': [3688], 'domain,': [3693], 'it': [3694, 3975], 'segment': [3707, 4078], 'acts': [3721], 'dissect': [3730], 'process,': [3745], 'additional': [3746, 4006], 'precise': [3750], 'deletions': [3751], 'constructed': [3753], '(with': [3754], 'tags': [3757], 'versions):': [3762], 'PdeCΔperi,': [3764], 'lacks': [3766], '(Thr44': [3771], 'Arg234)': [3773], 'thus': [3775, 4418], 'TM1': [3778, 3792, 3885, 3900, 4020], 'short': [3788], 'TM2;': [3794], '(beginning': [3799], 'initial': [3804, 3817], 'methionine);': [3805], 'alone': [3811, 3935, 3979, 4393], '[beginning': [3812], 'Arg263': [3814], 'Met-Ser': [3818], 'sequence,': [3819, 4444], 'serine': [3821], 'stability': [3825], 'according': [3826], 'N-end': [3829], 'rule': [3830], '(Mogk': [3831], '2007)].': [3834], 'immunoblots,': [3836], 'partially': [3838], 'deleted': [3839], 'constructs': [3841], 'appeared': [3842], 'fragments': [3845], 'sizes': [3849], '4A).': [3851], 'PdeCΔperi': [3853, 3881, 3944, 4014, 4317], 'confirms': [3871], 'latter': [3874], 'TM2.': [3876], 'integral': [3888], 'cleaved': [3895, 4052], 'signal': [3896], '(in': [3898, 3980], 'leader': [3908], 'peptidase': [3909], 'S3)': [3912], 'nor': [3913], 'generate': [3916], 'secAts': [3924], 'non-permissive': [3927], 'temperature;': [3928], 'S8).': [3931], 'soluble,': [3937], 'PdeCwt,': [3939], 'TM': [3948], 'found': [3958], 'Especially': [3966], 'EAL,': [3970], 'noteworthy,': [3973], 'because': [3974], 'means': [3976, 4411], 'TM1)': [3984], 'insert': [3986], 'membrane.': [3989], 'Interestingly,': [3990], '(since': [4001], 'inactive),': [4004], '4A):': [4013], 'shortened': [4017, 4307], 'get': [4025], 'removed,': [4027], 'general': [4032], 'minor': [4036], 'corresponding': [4038, 4211], 'almost': [4041, 4066], 'off': [4053], 'disappeared': [4068], 'cells.': [4072], '4.': [4074], '(TM2)': [4079], 'Immunoblot': [4094], 'analysis': [4095, 4209], 'tags.': [4108], '(A)': [4120], 'Protein–protein': [4137], 'interactions': [4138], 'two-hybrid': [4149, 4208, 4255], '(2H)': [4150], 'based': [4154], 'reconstitution': [4157], 'adenylate': [4159], 'cyclase': [4160], '(Karimova': [4161], '1998)': [4164], 'utilization': [4168], 'maltose': [4170], 'C-source': [4173], 'cya': [4176, 4248], '(resulting': [4181], 'color': [4184], 'MacConkey/maltose': [4186], 'plates).': [4188], 'positive': [4191], 'control,': [4192], 'leucine': [4194], 'zipper': [4195], "('zip')": [4197], 'yeast': [4200], 'GCN4': [4201], 'used.': [4204], 'variants,': [4213], 'LacY': [4225], '(TM2*),': [4226], 'S9.': [4230], 'assess': [4232], 'otherwise': [4244], 'isogenic': [4245], 'recipient': [4249], 'used': [4252], 'analysis.': [4256], 'information:': [4258], '2H': [4260], 'analyses,': [4261], 'compared': [4266], 'plate,': [4273], 'photographs': [4276], 'separately': [4279], 'achieve': [4281], 'higher': [4282], 'image': [4283], 'resolution.': [4284], '4': [4297], '[embj201797825-sup-0005-SDataFig4.jpg]': [4298], 'Phosphodiesterase': [4303], '4B).': [4316, 4409], 'PDE;': [4322], 'spatial': [4337], 'vicinity': [4338], 'inhibit': [4346], 'CR': [4361], 'white': [4370], 'color,': [4371], 'basal': [4377], 'red-binding': [4381], 'produced.': [4384], 'However,': [4385], 'pronounced': [4387], 'isolated': [4390], 'prevent': [4402], 'wrinkled': [4406], 'attachment': [4421], 'just': [4441], 'topogenic': [4443], 'ensures': [4446], 'proper': [4447], 'localization': [4448], 't': [4458]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2790198776', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 9}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 11}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 2}], 'updated_date': '2024-12-12T15:04:47.538509', 'created_date': '2018-03-29'}