Title: Hepatoprotective Activity of Basella Rubra Linn Against Ethanol Induced Hepatotoxicity in Male Wistar Albino Rats
Abstract: The present study was aimed to assess the hepatoprotective activity and diuretic activity
of methanolic and aqueous extract of Basella rubra leaves. LD50 studies were conducted in
albino rats with aqueous and methanolic extract of Basella rubra leaves according to OECD
guideline No.425 and was found safe upto the dose level of 4 gm/kg confirming its non-toxic
nature.
HEPATOPROTECTIVE ACTIVITY
The hepatoprotective activity was studied in ethanol induced hepatotoxic animal model. The
Physical parameter wet liver weight , Biochemical parameters like serum SGPT, SGOT, and
total bilirubin levels, and histopathology of livers were considered as major parameters of study.
Ethanol induced hepatotoxicity was significantly prevented by pretreatment ethanolic
extract of leaves. Decrease in wet liver weight, reduction in elevated biochemical parameter
levels like serum SGPT, SGOT, and total bilirubin, after treatment with ethanolic extract of
Basella rubra leaves confirmed the hepatoprotective effect of extract under study. In liver injury
models in rats restoration of hepatic cells with minor fatty changes and absence of necrosis after
treatment with extract was observed, indicating satisfactory hepatoprotection.
Based on improvement in serum marker enzyme levels, physical parameters, and
histopathological studies it was concluded that ethanol extract of Basella rubra leaves possesses
significant hepatoprotective activity in the doses used.
The hepatoprotective activity was studied in ethanol induced hepatotoxic animal model. The
Physical parameter wet liver weight , Biochemical parameters like serum SGPT, SGOT, and
total bilirubin levels, and histopathology of livers were considered as major parameters of study. CONCLUSION: The hepatoprotective effect of ethanolic extract of basella rubra leaves was confirmed by the
following measures: The isolated livers from the toxicant (ethanol) treated animals exhibited
increase in wet liver weight. Indeed, extract treated animals exhibited decrease in the values of
above physical parameters as an indication of hepatoprotection. Serum marker enzymes such as
SGPT, SGOT and total bilirubin, showed marked increase. The same is observed in liver
diseases in clinical practice and hence are having diagnostic importance in the assessment of
liver function. In the present study, the methanolic and aqueous extract of basella rubra leaves
significantly reduced the elevated levels of above mentioned serum marker enzymes. Hence, at
this point it is concluded that the methanolic and aqueous extract of basella rubra leaves
possesss hepatoprotective activity.
In support to this study, histopathological results also show significant activity of the
plant. In toxicant treated animals there will be severe disturbances in the cytoarchitecture of the
liver. The same is observed in case of humans who are suffering from major liver disorders. But
in the methanolic and aqueous extract of basella rubra leaves treated group animals exhibited
minimal hepatic derangements and intact cytoarchitecture of the liver was maintained. In
addition to this there is regeneration of hepatocytes also observed, which indicating
Hepatoprotective activity.
Finally based on improvement in serum marker enzyme levels, physical parameters,
functional parameters and histopathological studies, it is concluded that the ethanolic extract of
basella rubra leaves possesses hepatoprotective activity and thus supports the traditional
application of the same under the light of modern science.
Publication Year: 2017
Publication Date: 2017-10-01
Language: en
Type: dissertation
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