Title: FRET-Mediated Zn<sup>2+</sup> Sensing in Aqueous Micellar Solution: Application in Cellular Imaging and Molecular Logic Gate
Abstract: ChemistrySelectVolume 2, Issue 28 p. 8731-8737 Full Paper FRET-Mediated Zn2+ Sensing in Aqueous Micellar Solution: Application in Cellular Imaging and Molecular Logic Gate Shweta V. Pawar, Shweta V. Pawar Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorUday Kumar Togiti, Uday Kumar Togiti Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorPrakruti Trivedi, Prakruti Trivedi Department of Pharmacy, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Balaram Ghosh, Dr. Balaram Ghosh Department of Pharmacy, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Anupam Bhattacharya, Dr. Anupam Bhattacharya Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Amit Nag, Corresponding Author Dr. Amit Nag [email protected] Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this author Shweta V. Pawar, Shweta V. Pawar Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorUday Kumar Togiti, Uday Kumar Togiti Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorPrakruti Trivedi, Prakruti Trivedi Department of Pharmacy, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Balaram Ghosh, Dr. Balaram Ghosh Department of Pharmacy, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Anupam Bhattacharya, Dr. Anupam Bhattacharya Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this authorDr. Amit Nag, Corresponding Author Dr. Amit Nag [email protected] Department of Chemistry, BITS Pilani Hyderabad Campus, Hyderabad-, 500078 IndiaSearch for more papers by this author First published: 02 October 2017 https://doi.org/10.1002/slct.201701350Citations: 5Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Micelle templated FRET between 4-(pyridine-2-yl)-3H-pyrrolo [2, 3-c] quinoline (PPQ) and Rhodamine, enables easy detection and molecular imaging of intracellular Zn2+. The FRET dyad is selective for Zn2+ in presence of biological cations Na+, K+, Ca2+, Mg2+, Fe2+; however, Cd2+ and Hg2+ shows interference behaviours. Additionally, after careful observation on the fluorescence behaviour of PPQ, we propose that PPQ can also be used as a monomolecular circuit for an INHIBIT logic gate with Zn2+ and R6G as chemical inputs. Abstract Förster (fluorescence) resonance energy transfer (FRET) is an excellent method in generating characteristic fluorescence signals between two light sensitive molecules in the 1–10 nm range. On the other hand, studies of Zn2+ detection and imaging are also important, as zinc is the second most abundant transition metal ion in the human body and is involved in many biological activities and neurological functions. This work is a superposition between these two realities, which demonstrates the excellent performance of 4-(pyridine-2-yl)-3H-pyrrolo [2, 3-c] quinoline (PPQ) as a FRET sensor for Zn2+, entrapped in aqueous micellar solution, in presence of Rhodamine B (RB)/Rhodamine 6G (R6G). We show that easy, superior detection and molecular imaging of Zn2+ becomes possible via FRET, as PPQ nonradiatively transfers its energy to R6G/RB, only when it binds with Zn2+, over important biological cations including Na+, K+, Ca2+, Mg2+. During FRET, the Förster radius (R0) between the donor (PPQ) and the acceptor (RB/R6G) is calculated to be ≈ 4 nm, when they are encapsulated in sodium dodecyl sulphate (SDS) micelles at pH 6. Moreover, on careful observation of the fluorescence behavior of PPQ, we also demonstrate that PPQ functions like a monomolecular circuit in an INHIBIT logic gate, based upon two chemical inputs from Zn2+ and Rhodamine. Conflict of interest The authors declare no conflict of interest. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description slct201701350-sup-0001-misc_information.pdf1.1 MB Supplementary Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume2, Issue28September 29, 2017Pages 8731-8737 RelatedInformation
Publication Year: 2017
Publication Date: 2017-09-29
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 5
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